FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7687116
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6813738.863831377073014No Hit
CAACGGAATCCCAAAAGCAGCTG4671296.076778339236718No Hit
AAGCTGCCAGTTGAAGAACTGT3082584.01006047001242No Hit
CCCCCACTGCTAAATTTGACTG2729353.5505513381091167No Hit
TAGCAGCACGTAAATATTGGCG2698583.510523322400755No Hit
AACATTCAACGCTGTCGGTGAGT2367773.080179875001236No Hit
TTCAAGTAATCCAGGATAGGCT2365953.077812277062035No Hit
TAGCTTATCAGACTGATGTTGAC2140582.7846334047775523No Hit
TAGCTTATCAGACTGATGTTGACT1418781.845659672626249No Hit
AACATTCAACGCTGTCGGTGAG1219831.5868499967998402No Hit
TAGCTTATCAGACTGATGTTGA1118561.455110082897149No Hit
CATAAAGTAGAAAGCACTACT909891.1836558730218198No Hit
TGTAAACATCCTTGACTGGAAGCT736990.958734068797713No Hit
TATTGCACTTGTCCCGGCCTGTAA735650.9569908922930264No Hit
TATTGCACTTGTCCCGGCCTGT702350.9136716552735772No Hit
TATTGCACTTGTCCCGGCCTGTAT582390.7576183317644745No Hit
TTCAAGTAATTCAGGATAGGTT508480.6614704396291144No Hit
TGAGGTAGTAGGTTGTATAGTT454930.5918084233410814No Hit
TATTGCACTTGTCCCGGCCTGTA439450.5716708320779861No Hit
AGCAGCATTGTACAGGGCTATGA437570.5692251814594707No Hit
CCCATAAAGTAGAAAGCACTAC335380.43628845980729314No Hit
TGAGGTAGTAGATTGTATAGTT315010.4097895751800805No Hit
TGTAAACATCCCCGACTGGAAGCT304630.3962864616587027No Hit
ACTGGACTTGGAGTCAGAAGGC298720.38859827274624187No Hit
CAACGGAATCCCAAAAGCAGCTGT289450.376539133792179No Hit
TTAATGCTAATCGTGATAGGGGTT265840.345825404481993No Hit
CATTGCACTTGTCTCGGTCTGA265820.34579938692222156No Hit
TGTAAACATCCTACACTCTCAGCT260800.3392689794195899No Hit
AACATTCAACGCTGTCGGTGAGTT254630.3312425622301003No Hit
GGAATACCGGGTGCTGTAGGCTTT251910.32770417410118435No Hit
CGCCTGTCTGAGCGTCGCT232710.3027273167206011No Hit
TAGCTTATCAGACTGATGTTGACA232590.3025712113619724No Hit
TTCTCACTACTGCACTTGACTAG219900.28606306968699313No Hit
TTTAAACATCCTTGACTGGAAGCT211270.2748364926456164No Hit
CCCATAAAGTAGAAAGCACT205930.26788980418664166No Hit
CAACGGAATCCCAAAAGCAGCT188770.2455667379027453No Hit
GGAATACCGGGTGCTGTAGGCTT181560.23618740760514087No Hit
CGCCCCACTGCTAAATTTGACTG181220.23574510908902635No Hit
CAAAGAATTCTCCTTTTGGGCT177720.2311920361290242No Hit
CTTCTCACTACTGCACTTGACTAG171820.2235168559964491No Hit
TCGTACGACTCTTAGCGG171590.22321765405907754No Hit
CAACACCAGTCGATGGGCTGT166760.21693441337427458No Hit
TTATAAAGCAATGAGACTGATT162500.21139267314295765No Hit
TTCACAGTGGCTAAGTTCCG153000.19903433225152317No Hit
TTCAAGTAATCCAGGATAGGCTT145440.18919969465791853No Hit
TAGCAGCACGTAAATATTGGC143660.18688413183826028No Hit
TGAATACCGGGTGCTGTAGGCTTT142530.18541413971117385No Hit
TGATGTAGTAGGTTGTATAGTT141730.18437343732031622No Hit
ACTTGACTTGGAGTCAGAAGGC132080.171819964730596No Hit
CCCGCCACTGCTAAATTTGACTG130520.1697905950684236No Hit
CAAAGAATTCTCCTTTTGGGCTT130380.1696084721500235No Hit
CTCCCACTGCTTCACTTGACTAG127520.16588796110270745No Hit
TGAGGTAGTAGTTTGTACAGTT124900.16247966077264867No Hit
TATTGCACTTGTCCCGGCCTGTT123380.16050232623001917No Hit
CTACGCCTGTCTGAGCGTCGCT121610.15819977219024664No Hit
CCCCCCACTGCTAAATTTGACTGG114020.1483261082569848No Hit
TGTAAACATCCTACACTCAGCT112780.14671301955115548No Hit
ATCAGCATTGTACAGGGCTATGA112220.14598452787755511No Hit
TGCAAGTAATCCAGGATAGGCT108700.1414054373577815No Hit
CCCCCACTGCTAAATTTGACTGGCTT106550.13860854968235162No Hit
CACCCGGCTGTGTGCACATGTGC105900.13776297898977977No Hit
TGTCAGTTTGTCAAATACCCCA104080.13539538105057866No Hit
CCCTCCACTGCTAAATTTGACTG103900.1351612230126357No Hit
TGAATACCGGGTGCTGTAGGCTT103480.13461485425743544No Hit
TGAGGTAGTAGTTTGTGCTGTT102510.13335300260852054No Hit
TTCAAGTAATCCAGGATAGGC101730.13233831777743435No Hit
TGATGTAGTAGATTGTATAGTT99310.12919019304509No Hit
TACGCCTGTCTGAGCGTCGCT98380.127980376515718No Hit
ACCACAGGGTAGAACCACGGAC97090.12630224391046005No Hit
ACCCCCCACTGCTAAATTTGACTG96640.12571684881560263No Hit
TAGCAGCACGTAAATATTGGCGT95430.12414278644943046No Hit
TTTAAACATCCCCGACTGGAAGCT89840.11687087849331271No Hit
AGCTCGGTCTGAGGCCCCTCAGT89270.11612937803982665No Hit
CCCCCCACTGCTAAATTTGACTGGCT88260.11481549127136886No Hit
AACGGAATCCCAAAAGCAGCTG88200.11473743859205456No Hit
CACTAGATTGTGAGCTCCTGGA84730.11022339197170955No Hit
CAAAGTGCTGTTCGTGCAGGTAG84550.10998923393376657No Hit
TGTCAGTTTGTCAAATACCCCAA84330.10970304077628072No Hit
AGCAGCATTGTACAGGGCTATGT83730.10892251398313751No Hit
TTAGGTAGTAGGTTGTATAGTT83700.10888348764348033No Hit
TATTGCACTTGTCCCGGCCTGTAG82440.10724438137787956No Hit
GACCCGCGGGCGCTCTCCAGTCCT80670.10494182733810703No Hit
ACGCCTGTCTGAGCGTCGCT78490.10210591332301998No Hit
TTCACAGTGGCTAAGTTCTG77950.10140343920919107No Hit
TTATGTAGTAGGTTGTATAGTT77700.10107821971204806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)