FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9526613
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG117766112.36180161826664No Hit
CAACGGAATCCCAAAAGCAGCTG7592297.969558540900108No Hit
CCCCCACTGCTAAATTTGACTG4293614.506963807598776No Hit
TAGCAGCACGTAAATATTGGCG2955213.102057362884375No Hit
AACATTCAACGCTGTCGGTGAGT2549072.67573585701445No Hit
TTCAAGTAATCCAGGATAGGCT2423852.54429354902944No Hit
AACATTCAACGCTGTCGGTGAG2069592.1724300126393294No Hit
AAGCTGCCAGTTGAAGAACTGT2017592.1178460802385906No Hit
TATTGCACTTGTCCCGGCCTGTAT2000512.099917357826963No Hit
TATTGCACTTGTCCCGGCCTGTAA1942552.0390772670202937No Hit
TAGCTTATCAGACTGATGTTGACT1561911.6395228818468852No Hit
TAGCTTATCAGACTGATGTTGAC1498231.572678558476134No Hit
TATTGCACTTGTCCCGGCCTGT1108621.1637084449635984No Hit
TAGCTTATCAGACTGATGTTGA993411.0427735439657306No Hit
TATTGCACTTGTCCCGGCCTGTA975641.0241205347587858No Hit
TGAGGTAGTAGGTTGTATAGTT926320.9723497742587004No Hit
TGTAAACATCCTTGACTGGAAGCT888380.932524497426315No Hit
TGAGGTAGTAGATTGTATAGTT752300.7896825450976124No Hit
GCCTGTCTGAGCGTCGCT704650.7396647685803969No Hit
CATAAAGTAGAAAGCACTACT699070.7338074927573944No Hit
TTCAAGTAATTCAGGATAGGTT630230.6615467637868778No Hit
AGCAGCATTGTACAGGGCTATGA618930.6496852553997943No Hit
TGTAAACATCCCCGACTGGAAGCT543570.5705805410590311No Hit
CAACGGAATCCCAAAAGCAGCTGT410970.43139151343714705No Hit
CATTGCACTTGTCTCGGTCTGA380260.39915550259048No Hit
ACTGGACTTGGAGTCAGAAGGC376830.3955550624340466No Hit
TTCTCACTACTGCACTTGACTAG349360.3667200504523486No Hit
CCCATAAAGTAGAAAGCACTAC309360.32473241014408794No Hit
CCCATAAAGTAGAAAGCACT274710.28836061672705715No Hit
TATTGCACTTGTCCCGGCCTGTAG272670.28621924707133584No Hit
CCCCCACTGCTAAATTTGACTGGCTT271580.28507508387293573No Hit
CTCCCACTGCTTCACTTGACTAG263530.27662507126089825No Hit
GGAATACCGGGTGCTGTAGGCTTT256190.26892033926433245No Hit
TGTAAACATCCTACACTCTCAGCT254270.26690493252953595No Hit
CACTAGATTGTGAGCTCCTGGAA253010.2655823218598257No Hit
CAAAGAATTCTCCTTTTGGGCT252650.2652044330970514No Hit
TATTGCACTTGTCCCGGCCTGTT248670.26102666288637943No Hit
TGATGTAGTAGGTTGTATAGTT244730.25689088031601576No Hit
CTTCTCACTACTGCACTTGACTAG243740.25585168621838633No Hit
TGAGGTAGTAGTTTGTACAGTT243490.2555892634664597No Hit
CACCCGGCTGTGTGCACATGTGC237550.249354098880683No Hit
GACCCGCGGGCGCTCTCCAGTCCT227740.23905663009508205No Hit
TAGCTTATCAGACTGATGTTGACA227260.23855277841138292No Hit
AGCTCGGTCTGAGGCCCCTCAGT225340.23653737167658642No Hit
TCCTGTCTGAGCGTCGCT221510.23251705511707046No Hit
ACCCCCCACTGCTAAATTTGACTG218700.22956742338541514No Hit
CACTAGATTGTGAGCTCCTGGA217060.2278459301327765No Hit
CAACGGAATCCCAAAAGCAGCT214660.2253266717142808No Hit
TATTGCACTTGTCCCGGCCTGTTT203350.21345466641712013No Hit
TGATGTAGTAGATTGTATAGTT197160.20695707907941677No Hit
AACATTCAACGCTGTCGGTGAGTT196550.20631676756471584No Hit
TCGTACGACTCTTAGCGG196500.20626428301433047No Hit
TAGCAGCACGTAAATATTGGC183710.19283873502576415No Hit
TGTAAACATCCCCGACTGGAAGC179000.18789469037946646No Hit
CCCCCCACTGCTAAATTTGACTGGCT177470.18628866313767548No Hit
CAACACCAGTCGATGGGCTGT174640.18331803758586604No Hit
TATTGCACTTGTCCCGGCCTGTAAA166620.17489951570405976No Hit
GGAATACCGGGTGCTGTAGGCTT159240.1671527960671857No Hit
CAAAGAATTCTCCTTTTGGGCTT158850.16674341657418013No Hit
CCCCCCACTGCTAAATTTGACTGG158630.1665124845524847No Hit
TATTGCACTTGTCCCGGCCTGTAAT158620.16650198764240765No Hit
AACGGAATCCCAAAAGCAGCTG158070.16592465758816904No Hit
CCCCCCACTGCTAAATTTGACTGA153840.1614844646255705No Hit
TGTCAGTTTGTCAAATACCCCA146840.15413662757162488No Hit
TGAGGTAGTAGTTTGTGCTGTT139030.14593854080143698No Hit
TTCAAGTAATCCAGGATAGGCTT133330.13995530205750983No Hit
ATCCCGGACGAGCCCCCA132400.1389790894203428No Hit
TTATAAAGCAATGAGACTGATT129770.13621840207007466No Hit
ACTGGACTTGGAGTCAGAAGGCA129470.1359034947677627No Hit
AGAATTACTCTGAGACCT124800.13100143776177325No Hit
TATTGCACTTGTCCCGGCCTGA118110.12397900492021666No Hit
AGCAGCATTGTACAGGGCTATGT117960.12382155126906068No Hit
TTCAAGTAATCCAGGATAGGC114200.11987471308008417No Hit
TGTCAGTTTGTCAAATACCCCAA112040.11760738050343811No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC109470.11490967461363236No Hit
TCAGTGCACTACAGAACTTTGT108260.11363954849430748No Hit
CTCCCACTGCTTCACTTGACTAGCCT108130.11350308866330562No Hit
TTCACAGTGGCTAAGTTCCG107620.1129677462493753No Hit
ACTGGACTTGGAGTCAGAAGGCT104110.10928333081232543No Hit
CCCCCCACTGCTAAATTTGACTGT103200.1083281119953125No Hit
ACTTGACTTGGAGTCAGAAGGC101190.10621823306982241No Hit
ACCGGGTGCTGTAGGCTTA100040.10501108841095991No Hit
TGTCAGTTTGTCAAATACCCC97930.10279624038469916No Hit
TGAATACCGGGTGCTGTAGGCTTT97840.10270176819400557No Hit
AACCGGGCGGAAACACCA95520.10026648505612645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)