FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7526416
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG3680314.889857270711585No Hit
AAGCTGCCAGTTGAAGAACTGT3265744.339037331978461No Hit
CAACGGAATCCCAAAAGCAGCTG3149004.183930306270607No Hit
TAGCAGCACGTAAATATTGGCG3114894.138609930676168No Hit
TTCAAGTAATCCAGGATAGGCT2315803.0768960950338116No Hit
CATAAAGTAGAAAGCACTACT1867552.481327101770617No Hit
TAGCTTATCAGACTGATGTTGAC1855972.46594129264181No Hit
GCCTGTCTGAGCGTCGCT1768942.3503085665209045No Hit
CCCCCACTGCTAAATTTGACTG1750312.32555574924373No Hit
TAGCTTATCAGACTGATGTTGA1650462.1928896834828158No Hit
AACATTCAACGCTGTCGGTGAGT1393671.8517047157637845No Hit
TAGCTTATCAGACTGATGTTGACT1064701.4146175284491316No Hit
TGAGGTAGTAGGTTGTATAGTT865561.1500294429646194No Hit
TCCTGTCTGAGCGTCGCT836501.1114187682424144No Hit
AACATTCAACGCTGTCGGTGAG763191.014015170035778No Hit
TGTAAACATCCTTGACTGGAAGCT757061.0058705232344318No Hit
TGAGGTAGTAGATTGTATAGTT694660.9229625362190982No Hit
TTCAAGTAATTCAGGATAGGTT674460.8961237327301599No Hit
TATTGCACTTGTCCCGGCCTGT562880.7478725597947283No Hit
ACCGGGTGCTGTAGGCTT478630.6359334907876472No Hit
AGCAGCATTGTACAGGGCTATGA474370.6302734262894849No Hit
CGCCTGTCTGAGCGTCGCT441640.5867865927155766No Hit
TCGTACGACTCTTAGCGG412830.5485080814028881No Hit
TATTGCACTTGTCCCGGCCTGTAA376220.4998660717132829No Hit
CATTGCACTTGTCTCGGTCTGA346390.4602323336897668No Hit
TATTGCACTTGTCCCGGCCTGTAT344680.4579603359686735No Hit
TTATAAAGCAATGAGACTGATT316230.42016013996568885No Hit
TGAGGTAGTAGTTTGTACAGTT294760.3916339463564065No Hit
ACTGGACTTGGAGTCAGAAGGC279040.3707475111660052No Hit
TTCTCACTACTGCACTTGACTAG268730.35704909215754216No Hit
CAACACCAGTCGATGGGCTGT256670.34102552928246327No Hit
TATTGCACTTGTCCCGGCCTGTA251720.33444869377403535No Hit
ACCGGGTGCTGTAGGCTTT246760.3278585717292268No Hit
TGTAAACATCCCCGACTGGAAGCT222090.2950806864781325No Hit
TGAGGTAGTAGTTTGTGCTGTT213830.2841060074277053No Hit
TGTAAACATCCTACACTCTCAGCT212300.28207316736146393No Hit
TTCACAGTGGCTAAGTTCCG191020.25379941794341426No Hit
TGATGTAGTAGGTTGTATAGTT190910.25365326604322697No Hit
ACCGGGTGCTGTAGGCTTA178440.23708495517654085No Hit
CAACGGAATCCCAAAAGCAGCTGT176380.23434792868212442No Hit
CCCATAAAGTAGAAAGCACTAC168100.22334667655893586No Hit
CTTCTCACTACTGCACTTGACTAG167750.22288164778561267No Hit
TGATGTAGTAGATTGTATAGTT155410.2064860618918752No Hit
TGTAAACATCCTACACTCAGCT152310.20236723561386985No Hit
CCCATAAAGTAGAAAGCACT150500.19996237252896998No Hit
CAACGGAATCCCAAAAGCAGCT149210.19824840933586452No Hit
AACCGGGCGGAAACACCA139600.1854800478740479No Hit
TGTCAGTTTGTCAAATACCCCA135290.17975355069398238No Hit
TTCAAGTAATCCAGGATAGGCTT133900.1779067221370703No Hit
TAGCAGCACGTAAATATTGGC131480.17469138033294998No Hit
TAGCTTATCAGACTGATGTTGACA130730.17369489010440028No Hit
CTACGCCTGTCTGAGCGTCGCT122110.16224189574426925No Hit
TTCACAGTGGCTAAGTTCCGC121980.16206917077132066No Hit
TACGCCTGTCTGAGCGTCGCT121310.16117897283381624No Hit
TTCAAGTAATCCAGGATAGGC118760.15779090605674734No Hit
TAGCAGCACATAATGGTTTGT118330.15721958499237884No Hit
CGCGACCTCAGATCAGACGTGG116710.15506716609871152No Hit
TAGCAGCACGTAAATATTGGCGT113950.15140008205764868No Hit
AACATTCAACGCTGTCGGTGAGTT113540.1508553340660415No Hit
GGAATACCGGGTGCTGTAGGCTTT113060.15021758031976973No Hit
TGTCAGTTTGTCAAATACC111330.14791900952591513No Hit
AGCAGCATTGTACAGGGCTATG109560.14556729258653786No Hit
TCAGTGCACTACAGAACTTTGT107360.14264425458279212No Hit
AGCAGCATTGTACAGGGCTATGT106510.14151489899043582No Hit
ACGCCTGTCTGAGCGTCGCT105970.14079742602588005No Hit
CGCGACCTCAGATCAGACGTGGCG104430.138751299423258No Hit
CAAAGAATTCTCCTTTTGGGCT103650.13771494958556638No Hit
GGAATACCGGGTGCTGTAGGCTT101710.13513736152771785No Hit
CACCCGGCTGTGTGCACATGTGC100850.1339947193989809No Hit
TTCACAGTGGCTAAGTTCTGC100830.13396814632621953No Hit
CAAAGAATTCTCCTTTTGGGCTT97590.1296633085388849No Hit
TAGCTTATCAGACTGATGTTG97310.12929128552022637No Hit
CATAAAGTAGAAAGCACTAC94540.12561091494278287No Hit
ACCTGGTGCTGTAGGCTT94180.12513259963307902No Hit
TTCACAGTGGCTAAGTTCTG93070.12365779409482548No Hit
CTCCCACTGCTTCACTTGACTAG93060.1236445075584448No Hit
TGTCAGTTTGTCAAATACCCC92630.12307318649407632No Hit
GCGTTCGCGCTTTCCCCTG92080.1223424269931399No Hit
CAAAGTGCTGTTCGTGCAGGT90330.12001728312652396No Hit
TGAGGTAGTAGGTTGTATAGT89610.11906065250711627No Hit
TGTCAGTTTGTCAAATACCCCAA87450.11619076064889318No Hit
TCGACCGGACCTCGACCGGCT86450.11486210701082693No Hit
TTATAAAGCAATGAGACTGAT85480.11357331298190267No Hit
ACTGGACTTGGAGTCAGAAGG84810.11268311504439828No Hit
AACGGAATCCCAAAAGCAGCTG84770.11262996889887564No Hit
CATAAAGTAGAAAGCACTACTA84360.11208522090726848No Hit
TAGTGGGTTATCAGAACT82180.10918875597628407No Hit
TGTCAGTTTGTCAAATACCC80490.10694333132795211No Hit
ACCACAGGGTAGAACCACGGAC79520.10565453729902785No Hit
TATTGCACTTGTCCCGGCCTGTT78160.10384756835125776No Hit
TGAGGTAGTAGATTGTATAGT76870.1021336051581523No Hit
CAGTGCAATAGTATTGTCAAAGC76540.10169514945759044No Hit
TGTAAACATCCTTGACTGGAAGC76320.10140284565721587No Hit
TGAGGTAGTAAGTTGTATTGTT75500.10031334967400155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)