FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7272109
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5235057.198805738472842No Hit
CCCCCCACTGCTAAATTTGACTG4061225.5846522652507No Hit
AAGCTGCCAGTTGAAGAACTGT2267003.1173900171188302No Hit
TAGCAGCACGTAAATATTGGCG2068692.844690584258294No Hit
TAGCTTATCAGACTGATGTTGAC1868922.5699834807206545No Hit
TTCAAGTAATCCAGGATAGGCT1834602.5227894686397025No Hit
CCCCCACTGCTAAATTTGACTG1615162.2210338156372518No Hit
AACATTCAACGCTGTCGGTGAGT1320221.815456836524315No Hit
GCCTGTCTGAGCGTCGCT1044031.435663299326234No Hit
TGTAAACATCCTTGACTGGAAGCT787051.0822857578179865No Hit
TAGCTTATCAGACTGATGTTGACT765741.0529820166336892No Hit
AACATTCAACGCTGTCGGTGAG754941.038130754090732No Hit
TAGCTTATCAGACTGATGTTGA717200.9862338422045104No Hit
TATTGCACTTGTCCCGGCCTGT701180.9642044694324576No Hit
AGCAGCATTGTACAGGGCTATGA647990.8910620014083946No Hit
TGAGGTAGTAGGTTGTATAGTT645480.8876104579840594No Hit
CATAAAGTAGAAAGCACTACT523120.7193511538399657No Hit
ACTGGACTTGGAGTCAGAAGGC475190.6534418007210838No Hit
ACCGGGTGCTGTAGGCTT452270.6219241213243641No Hit
TGTAAACATCCCCGACTGGAAGCT415740.5716911008897144No Hit
TTCAAGTAATTCAGGATAGGTT404910.5567985848396937No Hit
CCCATAAAGTAGAAAGCACTAC364480.501202608486754No Hit
CCCCCACTGCTAAATTTGACTGGCTT352230.4843574264357149No Hit
TGAGGTAGTAGATTGTATAGTT351540.4834085957732482No Hit
TATTGCACTTGTCCCGGCCTGTA318540.43802973800310196No Hit
TATTGCACTTGTCCCGGCCTGTAA316290.43493572497331934No Hit
TATTGCACTTGTCCCGGCCTGTAT288480.39669372392520513No Hit
CAACGGAATCCCAAAAGCAGCT272170.374265567251536No Hit
CAACGGAATCCCAAAAGCAGCTGT271160.37287669918038907No Hit
CATTGCACTTGTCTCGGTCTGA269370.37041523992558417No Hit
TAGCTTATCAGACTGATGTTGACA257760.35445013269190545No Hit
TGAGGTAGTAGTTTGTGCTGTT249290.34280289253090124No Hit
TTCTCACTACTGCACTTGACTAG244610.3363673454289533No Hit
TTAATGCTAATCGTGATAGGGGTT225210.3096900775277158No Hit
TCGTACGACTCTTAGCGG212640.2924048580679965No Hit
CCCCCCACTGCTAAATTTGACTGGCT212600.2923498533919115No Hit
ACCGGGTGCTGTAGGCTTT206820.2844016776976253No Hit
TAGCAGCACGTAAATATTGGC202890.2789974682722715No Hit
CCCCCCACTGCTAAATTTGACTGG200930.27630223914410523No Hit
CGCCTGTCTGAGCGTCGCT194850.26794152837918134No Hit
CTTCTCACTACTGCACTTGACTAG190810.2623860560945937No Hit
GGGCTACGCCTGTCTGAGCGTCGCT182820.2513988720466099No Hit
TGTAAACATCCTACACTCTCAGCT182760.25131636503248234No Hit
AACATTCAACGCTGTCGGTGAGTT158830.21840981756461572No Hit
TAGCAGCACGTAAATATTGGCGT148670.2044386298390192No Hit
TGAGGTAGTAGTTTGTACAGTT142420.19584414920073395No Hit
AACGGAATCCCAAAAGCAGCTG141500.19457904165077833No Hit
CCCATAAAGTAGAAAGCACT137910.18964237197214728No Hit
CTCCCACTGCTTCACTTGACTAG137610.1892298369015096No Hit
CAAAGAATTCTCCTTTTGGGCT136610.18785471999938397No Hit
AGCAGCATTGTACAGGGCTATG135930.1869196405059385No Hit
TGTCAGTTTGTCAAATACCCCA133730.1838943833212621No Hit
CAAAGTGCTGTTCGTGCAGGTAG125350.17237090368144922No Hit
TCGTTTCCCGGCCAATGCACCA125320.17232965017438545No Hit
AGCAGCATTGTACAGGGCTATGT121440.16699419659413794No Hit
CAACACCAGTCGATGGGCTGT119590.1644502303252055No Hit
CTACGCCTGTCTGAGCGTCGCT118050.16233255029593205No Hit
TTCAAGTAATCCAGGATAGGCTT115120.1583034577727039No Hit
AGCTCGGTCTGAGGCCCCTCAGT111210.15292675068539263No Hit
TATTGCACTTGTCCCGGCCTGTT110630.15212918288215976No Hit
TACGCCTGTCTGAGCGTCGCT109900.15112534754360804No Hit
TTCACAGTGGCTAAGTTCCGC108990.14987399116267372No Hit
CTCCCACTGCTTCACTTGACTAGCCT108190.1487738976409732No Hit
CACCCGGCTGTGTGCACATGTGC105070.1444835329063412No Hit
TGAGGTAGTAGGTTGTGTGGTT102960.1415820362428561No Hit
ACCGGGTGCTGTAGGCTTA101330.1393405956923913No Hit
CGCGACCTCAGATCAGACGTGG101240.13921683517119998No Hit
TAGCAGCACGTAAATATTGGCGA99410.13670037124031006No Hit
TGTCAGTTTGTCAAATACCCCAA99090.13626033383162986No Hit
GGAATACCGGGTGCTGTAGGCTT98310.13518774264797184No Hit
TGTAAACATCCTACACTCAGCT97700.13434892133767523No Hit
CAAAGAATTCTCCTTTTGGGCTT95710.13161243870244518No Hit
TTATAAAGCAATGAGACTGATT93860.12906847243351274No Hit
TGTAAACATCCCCGACTGGAAGC91250.1254794173189648No Hit
TGTGCAAATCCATGCAAAACTG91160.12535565679777352No Hit
GCGTTCGCGCTTTCCCCTG90800.12486061471300829No Hit
ATCACATTGCCAGGGATTTCC89360.12288044637394736No Hit
CGCGACCTCAGATCAGACGTGGCG88810.12212413207777827No Hit
ACCCCCCACTGCTAAATTTGACTG87540.12037773361207868No Hit
GACCCGCGGGCGCTCTCCAGTCCT84480.11616987589157424No Hit
GTCAGGATGGCCGAGCGGTCTAAGG83680.11506978236987372No Hit
TTCAAGTAATCCAGGATAGGC82450.11337838858025918No Hit
AACATTCATTGCTGTCGGTGGGTT80680.11094443166349678No Hit
GGGAATACCGGGTGCTGTAGGCTT79850.10980308463473251No Hit
ACCACAGGGTAGAACCACGGAC78540.10800168149294792No Hit
GGAATACCGGGTGCTGTAGGCTTT78070.10735537654894887No Hit
CCCCCACTGCTAAATTTGACTGGCT77710.10686033446418364No Hit
GTGCGGTAACGCGACCGATCCCGGA77530.10661281342180101No Hit
TTCACCACCTTCTCCACCCAGC77270.10625528302724836No Hit
ATCACATTGCCAGGGATTTCCA75770.1041926076740599No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT73040.1004385385312569No Hit
TTCACAGTGGCTAAGTTCCG72980.10035603151712935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)