FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6488558
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG3718615.731026832155928No Hit
CAACGGAATCCCAAAAGCAGCTG3163024.874765702949715No Hit
GCCTGTCTGAGCGTCGCT2856704.402673136311643No Hit
CCCCCACTGCTAAATTTGACTG1820952.8064016689070206No Hit
TAGCAGCACGTAAATATTGGCG1766352.7222535423124827No Hit
TTCAAGTAATCCAGGATAGGCT1738292.6790081864106017No Hit
AAGCTGCCAGTTGAAGAACTGT1708672.6333585983203047No Hit
CATAAAGTAGAAAGCACTACT1272441.961052055017463No Hit
TCCTGTCTGAGCGTCGCT1153591.777883468098767No Hit
TAGCTTATCAGACTGATGTTGA1129441.7406641044127216No Hit
TAGCTTATCAGACTGATGTTGAC1052331.6218241402789342No Hit
AACATTCAACGCTGTCGGTGAGT1015951.565756212705504No Hit
TGAGGTAGTAGGTTGTATAGTT937031.4441267227633627No Hit
AACATTCAACGCTGTCGGTGAG898401.3845911526104875No Hit
TAGCTTATCAGACTGATGTTGACT864391.3321758085540731No Hit
TGAGGTAGTAGATTGTATAGTT833721.2849079872600353No Hit
TATTGCACTTGTCCCGGCCTGT673651.0382121882859026No Hit
TTCAAGTAATTCAGGATAGGTT653751.0075428161388094No Hit
TATTGCACTTGTCCCGGCCTGTAT598280.9220538677468861No Hit
ACCGGGTGCTGTAGGCTT544960.8398784444864329No Hit
TATTGCACTTGTCCCGGCCTGTAA518650.79933014392412No Hit
TGTAAACATCCTTGACTGGAAGCT440140.6783325355186777No Hit
ACCGGGTGCTGTAGGCTTA391400.6032156913754951No Hit
TATTGCACTTGTCCCGGCCTGTA363660.5604635113071349No Hit
ACCGGGTGCTGTAGGCTTT328790.5067227571981325No Hit
TGAGGTAGTAGTTTGTACAGTT309300.47668526658773797No Hit
CATTGCACTTGTCTCGGTCTGA305470.4707825683302823No Hit
AGCAGCATTGTACAGGGCTATGA263930.40676218044132456No Hit
CAACACCAGTCGATGGGCTGT252620.3893314970753132No Hit
TTATAAAGCAATGAGACTGATT226700.3493842545601041No Hit
TTCTCACTACTGCACTTGACTAG195340.3010530228750363No Hit
TGTAAACATCCCCGACTGGAAGCT185080.2852405727127661No Hit
TGAGGTAGTAGTTTGTGCTGTT177840.2740824694793512No Hit
ACTGGACTTGGAGTCAGAAGGC175780.2709076500510591No Hit
TGATGTAGTAGGTTGTATAGTT160640.24757426842759206No Hit
CACCCGGCTGTGTGCACATGTGC154470.2380652218875134No Hit
TCGTACGACTCTTAGCGG152430.23492122594881634No Hit
TGATGTAGTAGATTGTATAGTT145880.22482653310643136No Hit
CAACGGAATCCCAAAAGCAGCTGT143000.22038795060474148No Hit
CCCATAAAGTAGAAAGCACTAC142290.21929371672411652No Hit
TGAGAACTGAATTCCATGGGTT134040.20657902726615068No Hit
ATCCCGGACGAGCCCCCA132180.20371244273380928No Hit
TCAGTGCACTACAGAACTTTGT129380.19939715419049964No Hit
TTCACAGTGGCTAAGTTCCGC119420.1840470563721554No Hit
TTCACAGTGGCTAAGTTCCG118900.18324564564268364No Hit
ACCTGGTGCTGTAGGCTT117000.1803174141311521No Hit
CGCCTGTCTGAGCGTCGCT112950.17407565748815068No Hit
TATTGCACTTGTCCCGGCCTGTT112090.17275024743556272No Hit
CCCATAAAGTAGAAAGCACT107080.165028963291998No Hit
TAGCAGCACGTAAATATTGGC105850.16313331868190128No Hit
AACCGGGCGGAAACACCA104540.16111438011342427No Hit
CTCCCACTGCTTCACTTGACTAG103420.15938826469610043No Hit
TGAGGTAGTAAGTTGTATTGTT103180.1590183828209596No Hit
TTAATGCTAATCGTGATAGGGGTT95750.14756745643639155No Hit
TGTAAACATCCTACACTCTCAGCT95100.14656569302455183No Hit
CAACGGAATCCCAAAAGCAGCT95060.1465040460453617No Hit
CTTCTCACTACTGCACTTGACTAG94410.14550228263352197No Hit
TTCACAGTGGCTAAGTTCTG93940.14477793062803784No Hit
TAGCTTATCAGACTGATGTTGACA92700.1428668742731436No Hit
TGTCAGTTTGTCAAATACCCCA92610.14272816856996579No Hit
TGAGGTAGTAGATTGTATAGT89950.13862864445382164No Hit
TGAGGTAGTAGGTTGTATAGT89880.1385207622402389No Hit
CAAAGAATTCTCCTTTTGGGCT89760.13833582130266847No Hit
TTCAAGTAATCCAGGATAGGCTT88580.1365172354165594No Hit
TTCAAGTAATCCAGGATAGGC86890.1339126505457761No Hit
ACCTGGTGCTGTAGGCTTA84020.12948947978888375No Hit
AACGGAATCCCAAAAGCAGCTG83530.12873430429380459No Hit
TTCACAGTGGCTAAGTTCTGC82950.12784042309554758No Hit
CACTAGATTGTGAGCTCCTGGA82750.12753218819959689No Hit
TGAGAACTGAATTCCATAGGCTGT76900.11851631749303929No Hit
CCCTGTCTGAGCGTCGCT76400.11774573025316257No Hit
TAGCTTATCAGACTGATGTTG73450.11319926553788992No Hit
TATTGCACTTGTCCCGGCCTGTAG72760.11213585514686006No Hit
TTATGTAGTAGGTTGTATAGTT72210.11128820918299567No Hit
TTAGGTAGTAGGTTGTATAGTT71200.1097316229584447No Hit
ACCTGGTGCTGTAGGCTTT70880.10923844712492359No Hit
CCCCCACTGCTAAATTTGACTGGCTT69950.10780515485875289No Hit
TAGTGGGTTATCAGAACT69820.10760480217638495No Hit
TTTAAACATCCTTGACTGGAAGCT67860.1045841001960682No Hit
TTAGGTAGTAGATTGTATAGTT65730.1013013985541934No Hit
CAAAGAATTCTCCTTTTGGGCTT65550.10102398714783778No Hit
TTATGTAGTAGATTGTATAGTT65320.10066951701749448No Hit
AACATTCAACGCTGTCGGTGAGTT65170.10043834084553147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)