FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11017820
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7428306.742077833909067No Hit
GCCTGTCTGAGCGTCGCT6419525.82648836158151No Hit
CAACGGAATCCCAAAAGCAGCTG5699135.172647583641773No Hit
TAGCAGCACGTAAATATTGGCG4199993.811997291660238No Hit
AAGCTGCCAGTTGAAGAACTGT3912853.5513831229771404No Hit
TTCAAGTAATCCAGGATAGGCT3043872.762679005465691No Hit
CCCCCACTGCTAAATTTGACTG2828252.566977859503967No Hit
TCCTGTCTGAGCGTCGCT2319052.104817468428419No Hit
AACATTCAACGCTGTCGGTGAGT2148461.9499864764535997No Hit
TAGCTTATCAGACTGATGTTGAC1825881.6572062349902248No Hit
CATAAAGTAGAAAGCACTACT1586461.4399037196105944No Hit
TAGCTTATCAGACTGATGTTGA1227991.114548976113242No Hit
AACATTCAACGCTGTCGGTGAG1166101.058376339421047No Hit
TAGCTTATCAGACTGATGTTGACT966880.8775601707052756No Hit
TGTAAACATCCTTGACTGGAAGCT963940.8748917662477694No Hit
TATTGCACTTGTCCCGGCCTGT750680.6813326048165609No Hit
TGAGGTAGTAGGTTGTATAGTT684680.6214296476072398No Hit
AGCAGCATTGTACAGGGCTATGA617020.5600200402620482No Hit
TTCAAGTAATTCAGGATAGGTT555300.5040016990656954No Hit
ACCGGGTGCTGTAGGCTT551930.500943017765765No Hit
TATTGCACTTGTCCCGGCCTGTAA521170.4730246092239663No Hit
TTCTCACTACTGCACTTGACTAG509320.46226930554320184No Hit
CGCCTGTCTGAGCGTCGCT509320.46226930554320184No Hit
TATTGCACTTGTCCCGGCCTGTAT473500.4297583369486886No Hit
TGAGGTAGTAGATTGTATAGTT456600.41441954942084736No Hit
TGTCAGTTTGTCAAATACCCCAA401590.3644913422074421No Hit
TGTAAACATCCCCGACTGGAAGCT386740.3510131768353449No Hit
TATTGCACTTGTCCCGGCCTGTA384630.34909809744577425No Hit
CCCATAAAGTAGAAAGCACTAC382260.34694703670962135No Hit
CATTGCACTTGTCTCGGTCTGA378820.3438248219702264No Hit
TGTAAACATCCTACACTCTCAGCT373120.33865138475669415No Hit
TGTCAGTTTGTCAAATACCCCA351880.31937352398205815No Hit
CAACGGAATCCCAAAAGCAGCTGT340530.3090720305831825No Hit
ACTGGACTTGGAGTCAGAAGGC325140.2951037501066454No Hit
ACCGGGTGCTGTAGGCTTT315870.28669010748042717No Hit
CTTCTCACTACTGCACTTGACTAG301480.27362944756766766No Hit
TTCACAGTGGCTAAGTTCCG292780.2657331486628026No Hit
CCCATAAAGTAGAAAGCACT267760.24302448215708733No Hit
TGTAAACATCCTACACTCAGCT254060.23059008043333434No Hit
TTAATGCTAATCGTGATAGGGGTT245050.2224124191536983No Hit
CCCCCACTGCTAAATTTGACTGGCTT238290.2162769041425618No Hit
CAACGGAATCCCAAAAGCAGCT219530.1992499423660942No Hit
AACCGGGCGGAAACACCA219480.19920456133790532No Hit
TGAGGTAGTAGTTTGTACAGTT219090.19885058931803207No Hit
CTACGCCTGTCTGAGCGTCGCT218720.19851476970943438No Hit
ACCGGGTGCTGTAGGCTTA216410.19641816620710814No Hit
GGGCTACGCCTGTCTGAGCGTCGCT209730.19035526084107382No Hit
AACATTCAACGCTGTCGGTGAGTT204320.18544503359103706No Hit
TAGCAGCACGTAAATATTGGC201280.18268586707715317No Hit
CAACACCAGTCGATGGGCTGT200970.18240450470238212No Hit
CCCCCCACTGCTAAATTTGACTGG200410.1818962371866667No Hit
CAAAGAATTCTCCTTTTGGGCT195770.17768487777073866No Hit
TGAGGTAGTAGTTTGTGCTGTT194990.17697693373099216No Hit
TGATGTAGTAGGTTGTATAGTT190300.17272019328687527No Hit
TAGCAGCACGTAAATATTGGCGT182420.16556814324430785No Hit
GGAATACCGGGTGCTGTAGGCTTT176100.1598319812812335No Hit
CTCCCACTGCTTCACTTGACTAG167320.15186307273126626No Hit
GGAATACCGGGTGCTGTAGGCTT166910.15149094830011745No Hit
TAGCTTATCAGACTGATGTTGACA166140.1507920804660087No Hit
TTATAAAGCAATGAGACTGATT164440.14924912550758682No Hit
CCCTGTCTGAGCGTCGCT160780.14592723424416082No Hit
TTCAAGTAATCCAGGATAGGC156330.14188832273535054No Hit
CACCCGGCTGTGTGCACATGTGC149440.13563481705092295No Hit
CCCCCCACTGCTAAATTTGACTGGCT147730.1340827858868633No Hit
TACGCCTGTCTGAGCGTCGCT144070.1307608946234373No Hit
AGCAGCATTGTACAGGGCTATG140500.12752068921075133No Hit
TGTAAACATCCCCGACTGGAAGC138810.1259868104579672No Hit
TGTCAGTTTGTCAAATACCCC137730.1250065802490874No Hit
CAAAGAATTCTCCTTTTGGGCTT131710.1195427044551463No Hit
ACGCCTGTCTGAGCGTCGCT130330.11829018807713322No Hit
ACCCCCCACTGCTAAATTTGACTG130320.11828111187149545No Hit
TGATGTAGTAGATTGTATAGTT129230.11729180545697789No Hit
TTCAAGTAATCCAGGATAGGCTT128150.11631157524809808No Hit
CGCGACCTCAGATCAGACGTGGCG127630.11583961255493373No Hit
TTCACAGTGGCTAAGTTCCGC126520.11483215372914062No Hit
ACCTGGTGCTGTAGGCTT123990.1125358737027833No Hit
TAGCAGCACATAATGGTTTGT122140.11085677565979477No Hit
AACGGAATCCCAAAAGCAGCTG119330.10830636187557975No Hit
CGCGACCTCAGATCAGACGTGG117230.10640035869164681No Hit
TATTGCACTTGTCCCGGCCTGTT116580.10581040532519137No Hit
TTCACAGTGGCTAAGTTCTGC116480.10571964326881363No Hit
AGCAGCATTGTACAGGGCTATGT111780.10145382661905895No Hit
ATCACATTGCCAGGGATTTCC110470.10026484368051031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)