FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7173652
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG76374710.646557708681714No Hit
CAACGGAATCCCAAAAGCAGCTG5289817.3739428675938No Hit
CCCCCACTGCTAAATTTGACTG3781075.2707742165357345No Hit
AACATTCAACGCTGTCGGTGAGT2115282.948679417401346No Hit
GCCTGTCTGAGCGTCGCT1769662.4668885527204276No Hit
AACATTCAACGCTGTCGGTGAG1762152.456419686932123No Hit
TAGCAGCACGTAAATATTGGCG1484982.070047445847666No Hit
TTCAAGTAATCCAGGATAGGCT1364301.9018207183732916No Hit
AAGCTGCCAGTTGAAGAACTGT1259351.7555214554595064No Hit
TAGCTTATCAGACTGATGTTGAC965111.3453538030559609No Hit
TGAGGTAGTAGGTTGTATAGTT789871.1010709747280742No Hit
TAGCTTATCAGACTGATGTTGA760941.0607428406061514No Hit
TATTGCACTTGTCCCGGCCTGTAA755761.0535219717934463No Hit
TAGCTTATCAGACTGATGTTGACT743831.036891669682332No Hit
TATTGCACTTGTCCCGGCCTGTAT604620.8428343053161764No Hit
TATTGCACTTGTCCCGGCCTGT602560.8399626856725138No Hit
TCGTACGACTCTTAGCGG559810.7803696081159219No Hit
CATAAAGTAGAAAGCACTACT558940.7791568367130159No Hit
ACCGGGTGCTGTAGGCTT491570.6852437224443003No Hit
TGAGGTAGTAGATTGTATAGTT442100.6162830312928478No Hit
TATTGCACTTGTCCCGGCCTGTA427620.5960980543801121No Hit
AGCAGCATTGTACAGGGCTATGA397080.5535255961677539No Hit
CATTGCACTTGTCTCGGTCTGA361730.5042480454864552No Hit
TGTAAACATCCTTGACTGGAAGCT351580.4900990457858842No Hit
CAACACCAGTCGATGGGCTGT313530.4370577217852218No Hit
TTCAAGTAATTCAGGATAGGTT306130.42674219490992876No Hit
CACCCGGCTGTGTGCACATGTGC298250.41575755277785986No Hit
ACTGGACTTGGAGTCAGAAGGC297900.4152696562364609No Hit
ACCGGGTGCTGTAGGCTTT294780.41092040706741834No Hit
TGTAAACATCCCCGACTGGAAGCT268810.3747184836956128No Hit
CAACGGAATCCCAAAAGCAGCT268540.37434210636367643No Hit
ACCGGGTGCTGTAGGCTTA255860.3566663116638499No Hit
TCCTGTCTGAGCGTCGCT250530.3492363443334023No Hit
CCCATAAAGTAGAAAGCACT239190.3334284963920748No Hit
CAACGGAATCCCAAAAGCAGCTGT197660.275536086779788No Hit
TGATGTAGTAGGTTGTATAGTT194070.2705316622551526No Hit
TTCTCACTACTGCACTTGACTAG171800.23948750232099356No Hit
CCCCCACTGCTAAATTTGACTGGCTT161410.22500394499203472No Hit
TGAGGTAGTAGTTTGTACAGTT158540.22100319335256297No Hit
CCCATAAAGTAGAAAGCACTAC153000.21328048809727598No Hit
CGCGACCTCAGATCAGACGTGG144340.2012085336729465No Hit
AACATTCAACGCTGTCGGTGAGTT140290.19556287369390093No Hit
AACGGAATCCCAAAAGCAGCTG137860.1921754777064736No Hit
GGAATACCGGGTGCTGTAGGCTT136500.19027965114560896No Hit
TGAGGTAGTAGTTTGTGCTGTT135060.18827230537528167No Hit
GGAATACCGGGTGCTGTAGGCTTT130810.18234784737257956No Hit
CACTAGATTGTGAGCTCCTGGA130690.1821805685583856No Hit
TTATAAAGCAATGAGACTGATT127750.17808223761063405No Hit
ACCCCCCACTGCTAAATTTGACTG119140.1660799826922187No Hit
CTTCTCACTACTGCACTTGACTAG118640.16538298763307727No Hit
TAGCAGCACGTAAATATTGGC118330.16495085069640958No Hit
CGCCTGTCTGAGCGTCGCT117000.1630968438390934No Hit
CGCGACCTCAGATCAGACGTGGCG112350.15661478978907814No Hit
AGAATTACTCTGAGACCT109760.15300435538272555No Hit
ACCTGGTGCTGTAGGCTT109590.15276737706261748No Hit
TGATGTAGTAGATTGTATAGTT109260.15230736032358413No Hit
TCGTTTCCCGGCCAATGCACCA109260.15230736032358413No Hit
TAGCTTATCAGACTGATGTTGACA105220.14667564024572144No Hit
CCCCCCACTGCTAAATTTGACTGG99830.13916203350817685No Hit
TGTAAACATCCTACACTCTCAGCT95220.13273573906289293No Hit
TATTGCACTTGTCCCGGCCTGTT94820.13217814301557979No Hit
CTCCCACTGCTTCACTTGACTAG94030.13107689082213636No Hit
CACTAGATTGTGAGCTCCTGGAA93770.1307144533913828No Hit
TATTGCACTTGTCCCGGCCTGTAG92870.12945986228492826No Hit
CAAAGAATTCTCCTTTTGGGCT91690.12781495394535447No Hit
GGGAATACCGGGTGCTGTAGGCTT87150.12148623880835034No Hit
TCAGTGCACTACAGAACTTTGT85480.11915827531081798No Hit
AGCAGCATTGTACAGGGCTATG85180.11874007827533313No Hit
AGCAGCATTGTACAGGGCTATGT84340.11756912657597553No Hit
TTCACAGTGGCTAAGTTCTG83280.11609149705059571No Hit
ACTGGACTTGGAGTCAGAAGG79660.11104525282241179No Hit
CCCGCCACTGCTAAATTTGACTG78250.10907972675563296No Hit
TGTCAGTTTGTCAAATACCCCA78080.1088427484355249No Hit
ACTGGACTTGGAGTCAGAAGGCT77390.10788089525390972No Hit
GACCCGCGGGCGCTCTCCAGTCCT76900.10719784009595114No Hit
ACTTGACTTGGAGTCAGAAGGC75940.10585960958239958No Hit
CCCATAAAGTAGAAAGCACTA74630.10403348252744907No Hit
TTCAAGTAATCCAGGATAGGCTT74390.10369892489906118No Hit
AACATTCAACGCTGTCGGTGA72410.10093882446486115No Hit
TTCAAGTAATCCAGGATAGGC71890.10021394960335406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)