FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7230915
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5171657.152137730840426No Hit
GCCTGTCTGAGCGTCGCT3489154.82532293630889No Hit
CCCCCCACTGCTAAATTTGACTG3418664.727838731336214No Hit
AAGCTGCCAGTTGAAGAACTGT2512893.475203345634681No Hit
TTCAAGTAATCCAGGATAGGCT2272453.1426866447745545No Hit
TAGCAGCACGTAAATATTGGCG1894662.6202216455317204No Hit
AACATTCAACGCTGTCGGTGAGT1610762.22760190100423No Hit
TCCTGTCTGAGCGTCGCT1565342.164788273683206No Hit
TAGCTTATCAGACTGATGTTGAC1398661.9342780270546673No Hit
CCCCCACTGCTAAATTTGACTG1316201.8202399004828573No Hit
TAGCTTATCAGACTGATGTTGACT1126011.5572164795188437No Hit
TGAGGTAGTAGGTTGTATAGTT1086901.5031292720215905No Hit
TAGCTTATCAGACTGATGTTGA1051411.454048346578545No Hit
AACATTCAACGCTGTCGGTGAG1049061.4507984120958413No Hit
TATTGCACTTGTCCCGGCCTGT687020.9501148886413407No Hit
CATAAAGTAGAAAGCACTACT612150.846573358973242No Hit
TCGTACGACTCTTAGCGG603700.8348874243439454No Hit
TATTGCACTTGTCCCGGCCTGTAA574580.7946158957752926No Hit
TGAGGTAGTAGATTGTATAGTT568050.7855852267659071No Hit
CGCCTGTCTGAGCGTCGCT547080.7565847475734399No Hit
TATTGCACTTGTCCCGGCCTGTAT481280.6655865820577341No Hit
TGTAAACATCCTTGACTGGAAGCT470360.6504847588444893No Hit
TTCAAGTAATTCAGGATAGGTT385100.5325743699103087No Hit
AGCAGCATTGTACAGGGCTATGA356100.4924687954429004No Hit
ACTGGACTTGGAGTCAGAAGGC334820.4630396014888849No Hit
TATTGCACTTGTCCCGGCCTGTA318830.44092621749806216No Hit
CAACGGAATCCCAAAAGCAGCTGT301310.41669691871637266No Hit
CAACACCAGTCGATGGGCTGT294350.4070715808441947No Hit
AACCGGGCGGAAACACCA261200.36122676037541585No Hit
CATTGCACTTGTCTCGGTCTGA253330.35034293723546744No Hit
ACCGGGTGCTGTAGGCTT247590.342404799392608No Hit
CCCATAAAGTAGAAAGCACTAC241980.33464644515943No Hit
TGTAAACATCCCCGACTGGAAGCT235680.3259338548440965No Hit
TTCTCACTACTGCACTTGACTAG233690.32318178266512604No Hit
CCCATAAAGTAGAAAGCACT219760.30391727741233304No Hit
CAACGGAATCCCAAAAGCAGCT216450.29933971011967364No Hit
TGAGGTAGTAGTTTGTACAGTT198210.2741146867305175No Hit
ACCGGGTGCTGTAGGCTTT191560.2649180636198877No Hit
TTAATGCTAATCGTGATAGGGGTT179890.24877902727386508No Hit
CTCCCACTGCTTCACTTGACTAG179090.24767266659890208No Hit
CACCCGGCTGTGTGCACATGTGC170490.23577928934304992No Hit
TACGCCTGTCTGAGCGTCGCT164220.22710818755302753No Hit
ACCGGGTGCTGTAGGCTTA161750.2236922989690793No Hit
CTACGCCTGTCTGAGCGTCGCT155240.21468928897656797No Hit
AACGGAATCCCAAAAGCAGCTG153580.21239359057601978No Hit
TAGCTTATCAGACTGATGTTGACA147090.2034182396003825No Hit
TGAGGTAGTAGTTTGTGCTGTT140160.19383439025351562No Hit
CCCCCACTGCTAAATTTGACTGGCTT139900.19347482303415267No Hit
CGCGACCTCAGATCAGACGTGGCG137050.189533413129597No Hit
CGCGACCTCAGATCAGACGTGG131520.18188569496391535No Hit
CTTCTCACTACTGCACTTGACTAG129280.178787885074019No Hit
ACGCCTGTCTGAGCGTCGCT127230.1759528358444263No Hit
AACATTCAACGCTGTCGGTGAGTT124560.17226035709173734No Hit
TTCAAGTAATCCAGGATAGGCTT122300.16913488818496691No Hit
TAGCAGCACGTAAATATTGGC121030.16737854061346316No Hit
AGCTCGGTCTGAGGCCCCTCAGT120890.16718492749534464No Hit
CCCTGTCTGAGCGTCGCT120280.16634132748068536No Hit
TTATAAAGCAATGAGACTGATT120050.1660232487866335No Hit
CCCCCCACTGCTAAATTTGACTGG113670.15720002240380365No Hit
TGTAAACATCCTACACTCTCAGCT112640.1557755830347888No Hit
TGAGGTAGTAGGTTGTGTGGTT111930.15479368793575918No Hit
TTCACAGTGGCTAAGTTCTGC111050.15357669119329986No Hit
TATTGCACTTGTCCCGGCCTGTT99110.13706425811947726No Hit
TTCAAGTAATCCAGGATAGGC98380.13605470400357356No Hit
CCCCCCACTGCTAAATTTGACTGGCT96390.1333026318246031No Hit
TTCACAGTGGCTAAGTTCCG93530.12934739241161042No Hit
ACCACAGGGTAGAACCACGGAC91610.12669212679169925No Hit
TGAGGTAGTAGGTTGTATAGT90640.12535066447330664No Hit
TTCACAGTGGCTAAGTTCCGC87510.12102202833251394No Hit
TAGTGGGTTATCAGAACT84530.1169008348182768No Hit
AATGCACCCGGGCAAGGATTCT84170.11640297251454346No Hit
TTCACAGTGGCTAAGTTCTG79290.10965417239726923No Hit
CAACGGAATCCCAAAAGCAGCTGA77600.10731698547140991No Hit
CAAAGTGCTGTTCGTGCAGGT77520.1072063494039136No Hit
AGAGGTAGTAGGTTGCATAGTT77380.10701273628579508No Hit
ACCCCCCACTGCTAAATTTGACTG77230.10680529365923953No Hit
TGATGTAGTAGGTTGTATAGTT77040.10654253299893582No Hit
CACTAGATTGTGAGCTCCTGGA75020.10374897229465427No Hit
CAAAGAATTCTCCTTTTGGGCT74880.10355535917653574No Hit
TGAGAACTGAATTCCATGGGTT73490.10163305750378755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)