FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7270600
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4029865.5426787335295575No Hit
CCCCCCACTGCTAAATTTGACTG3553624.887657139713366No Hit
AAGCTGCCAGTTGAAGAACTGT2761923.7987511347069023No Hit
GCCTGTCTGAGCGTCGCT2647363.6411850466261377No Hit
TTCAAGTAATCCAGGATAGGCT1874462.5781366049569496No Hit
AACATTCAACGCTGTCGGTGAGT1783802.453442631969851No Hit
TAGCAGCACGTAAATATTGGCG1643722.2607762770610402No Hit
TAGCTTATCAGACTGATGTTGAC1517132.08666409924903No Hit
CCCCCACTGCTAAATTTGACTG1147271.5779578026572774No Hit
TCGTACGACTCTTAGCGG1089621.4986658597639808No Hit
TAGCTTATCAGACTGATGTTGACT1056391.4529612411630402No Hit
TGAGGTAGTAGGTTGTATAGTT932831.2830165323357081No Hit
CGCCTGTCTGAGCGTCGCT922001.268120925370671No Hit
AACATTCAACGCTGTCGGTGAG912791.255453470140016No Hit
TAGCTTATCAGACTGATGTTGA910221.2519186862157181No Hit
TATTGCACTTGTCCCGGCCTGT782421.076142271614447No Hit
TATTGCACTTGTCCCGGCCTGTAA617640.8495034797678321No Hit
TGTAAACATCCTTGACTGGAAGCT611640.8412510659367866No Hit
TATTGCACTTGTCCCGGCCTGTAT547210.7526338954144087No Hit
TGAGGTAGTAGATTGTATAGTT545380.7501169091959398No Hit
TTCAAGTAATTCAGGATAGGTT533130.7332682309575551No Hit
AGCAGCATTGTACAGGGCTATGA455580.6266057821912909No Hit
ACCGGGTGCTGTAGGCTT405660.5579456991169917No Hit
AACCGGGCGGAAACACCA388090.53377988061508No Hit
CATAAAGTAGAAAGCACTACT378570.5206860506698209No Hit
ACTGGACTTGGAGTCAGAAGGC372370.5121585563777404No Hit
TATTGCACTTGTCCCGGCCTGTA342420.47096525733777134No Hit
TGTAAACATCCCCGACTGGAAGCT319440.4393585123648667No Hit
TACGCCTGTCTGAGCGTCGCT305680.42043297664566887No Hit
CTACGCCTGTCTGAGCGTCGCT293940.404285753582923No Hit
ACCGGGTGCTGTAGGCTTT283820.39036668225455945No Hit
TTCTCACTACTGCACTTGACTAG281990.3878496960360906No Hit
TTAATGCTAATCGTGATAGGGGTT260460.3582372844056887No Hit
ACCGGGTGCTGTAGGCTTA243850.33539185211674416No Hit
CAACGGAATCCCAAAAGCAGCTGT231460.31835061755563504No Hit
ACGCCTGTCTGAGCGTCGCT224360.30858526118889773No Hit
CTCCCACTGCTTCACTTGACTAG221220.3042664979506505No Hit
TGAGGTAGTAGTTTGTACAGTT220430.3031799301295629No Hit
TCCTGTCTGAGCGTCGCT218320.3002778312656452No Hit
CATTGCACTTGTCTCGGTCTGA217130.2986411025224878No Hit
CCCATAAAGTAGAAAGCACTAC209240.2877891783346629No Hit
CAACACCAGTCGATGGGCTGT208520.2867988886749374No Hit
GGAATACCGGGTGCTGTAGGCTTT199720.2746953483894039No Hit
GCGTTCGCGCTTTCCCCTG197950.27226088630924544No Hit
AGCTCGGTCTGAGGCCCCTCAGT189430.26054245866916076No Hit
GGAATACCGGGTGCTGTAGGCTT167660.230599950485517No Hit
TGAGGTAGTAGTTTGTGCTGTT164680.22650125161609772No Hit
TAGCTTATCAGACTGATGTTGACA161420.2220174401012296No Hit
CTTCTCACTACTGCACTTGACTAG159420.2192666354908811No Hit
AACATTCAACGCTGTCGGTGAGTT158420.21789123318570683No Hit
CCCATAAAGTAGAAAGCACT157980.21728605617143013No Hit
TGTAAACATCCTACACTCTCAGCT152750.21009270211536876No Hit
CCCCCCACTGCTAAATTTGACTGGCT144560.19882815723599154No Hit
CACCCGGCTGTGTGCACATGTGC139600.19200616180232719No Hit
CTCGACACAAGGGTTTGT138920.1910708882348087No Hit
GGGAATACCGGGTGCTGTAGGCTTT134250.18464775946964487No Hit
GGGAATACCGGGTGCTGTAGGCTT133000.18292850658817705No Hit
CCCCCACTGCTAAATTTGACTGGCTT132870.18274970428850437No Hit
CCTCGACACAAGGGTTTGT131660.1810854674992435No Hit
ATCCCGGACGAGCCCCCA127490.17535003988666684No Hit
TTCAAGTAATCCAGGATAGGCTT126510.17400214562759608No Hit
TGAGGTAGTAGGTTGTGTGGTT124130.17072868814128134No Hit
CCCCCCACTGCTAAATTTGACTGG120900.16628613869556846No Hit
AACGGAATCCCAAAAGCAGCTG111950.1539762880642588No Hit
TGATGTAGTAGGTTGTATAGTT110710.1522707892058427No Hit
TGTCAGTTTGTCAAATACCCCAA108150.1487497593045966No Hit
GGGCTACGCCTGTCTGAGCGTCGCT107950.14847467884356175No Hit
TTCACAGTGGCTAAGTTCCG106880.1470029983770253No Hit
TATTGCACTTGTCCCGGCCTGTT105960.14573762825626496No Hit
ACCACAGGGTAGAACCACGGAC103480.14232663053943279No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT102600.14111627651087943No Hit
TAGCAGCACGTAAATATTGGC100830.13868181443072097No Hit
CTCCCACTGCTTCACTTGACTAGCCT99720.13715511787197754No Hit
TTATAAAGCAATGAGACTGATT98490.1354633730366132No Hit
TGAGAACTGAATTCCATAGGCTGT96200.13231370175776414No Hit
GAATACCGGGTGCTGTAGGCTTT95870.13185981899705665No Hit
CGCGACCTCAGATCAGACGTGGCG91970.12649575000687702No Hit
TGAGGTAGTAGGTTGTATAGT91750.12619316149973867No Hit
AGCAGCATTGTACAGGGCTATGT90830.12492779137897835No Hit
TGTAAACATCCCCGACTGGAAGC89970.12374494539652849No Hit
CAAAGTGCTGTTCGTGCAGGTAG89770.12346986493549364No Hit
TGAGAACTGAATTCCATGGGTT88580.12183313619233627No Hit
AGAGGTAGTAGGTTGCATAGTT88480.12169559596181884No Hit
GACCCGCGGGCGCTCTCCAGTCCT87360.12015514538002367No Hit
CAAAGTGCTGTTCGTGCAGGT85780.11798200973784831No Hit
TTCAAGTAATCCAGGATAGGC85530.11763815916155476No Hit
CACTAGATTGTGAGCTCCTGGA84080.11564382581905207No Hit
CAACGGAATCCCAAAAGCAGCT83980.11550628558853464No Hit
TGTCAGTTTGTCAAATACCCCA82910.11403460512199817No Hit
CAAAGAATTCTCCTTTTGGGCT81790.11249415454020302No Hit
ATACCGGGTGCTGTAGGCTT78910.10853299590130112No Hit
TACCTGGTTGATCCTGCC78370.10779027865650702No Hit
AGCAGCATTGTACAGGGCTATG78150.1074876901493687No Hit
GAATACCGGGTGCTGTAGGCTT76860.10571342117569389No Hit
CAAAGAATTCTCCTTTTGGGCTT75480.1038153659945534No Hit
TATTGCACTTGTCCCGGCCTGTAG73490.10107831540725662No Hit
ACCCCCCACTGCTAAATTTGACTG73330.10085825103842874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)