FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825166
Sequences flagged as poor quality0
Sequence length18-26
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT219201628.012389769111607No Hit
CAACGGAATCCCAAAAGCAGCTG4916016.282307621333529No Hit
TCCTGTCTGAGCGTCGCT2842853.632958073988463No Hit
CCCCCCACTGCTAAATTTGACTG2829733.6161916565092675No Hit
TTCAAGTAATCCAGGATAGGCT1770282.262290665782681No Hit
TAGCAGCACGTAAATATTGGCG1317931.6842198619173063No Hit
CCCCCACTGCTAAATTTGACTG1298021.6587763122213637No Hit
AAGCTGCCAGTTGAAGAACTGT1050641.3426424436235602No Hit
AACATTCAACGCTGTCGGTGAGT912461.1660583302641758No Hit
TAGCTTATCAGACTGATGTTGA702340.8975400649647559No Hit
TGAGGTAGTAGGTTGTATAGTT689970.8817320936067043No Hit
AACATTCAACGCTGTCGGTGAG678590.8671892711285614No Hit
TAGCTTATCAGACTGATGTTGAC614770.7856318958601007No Hit
TCGTACGACTCTTAGCGG539330.6892249953547311No Hit
CATAAAGTAGAAAGCACTACT529050.6760878938542645No Hit
TAGCTTATCAGACTGATGTTGACT527400.67397931238775No Hit
ACCGGGTGCTGTAGGCTT463750.5926391849067484No Hit
CGCCTGTCTGAGCGTCGCT439770.5619944675934031No Hit
CCCTGTCTGAGCGTCGCT388420.4963728564991465No Hit
CAACACCAGTCGATGGGCTGT343490.4389555442018738No Hit
AACCGGGCGGAAACACCA328750.42011888310101025No Hit
TGAGGTAGTAGATTGTATAGTT310750.39711617619357853No Hit
TATTGCACTTGTCCCGGCCTGTAA309940.3960810543827441No Hit
ACCGGGTGCTGTAGGCTTT305870.3908798867653415No Hit
TATTGCACTTGTCCCGGCCTGT298160.38102706063999153No Hit
TTCAAGTAATTCAGGATAGGTT266210.3401972558793002No Hit
CAACGGAATCCCAAAAGCAGCTGT263610.33687464265933786No Hit
ACCGGGTGCTGTAGGCTTA243440.311098831641399No Hit
CATTGCACTTGTCTCGGTCTGA231550.2959042658008788No Hit
TATTGCACTTGTCCCGGCCTGTAT229580.2933867473226766No Hit
TCCGGTCTGAGCGTCGCT220870.2822559930358027No Hit
CCCATAAAGTAGAAAGCACTAC211510.27029458544393814No Hit
CCCATAAAGTAGAAAGCACT209670.267943197626734No Hit
AGCAGCATTGTACAGGGCTATGA194300.2483014417841104No Hit
GCCGGTCTGAGCGTCGCT186170.2379118858309204No Hit
ACTGGACTTGGAGTCAGAAGGC173940.22228282441548206No Hit
TTAATGCTAATCGTGATAGGGGTT173330.22150328823695242No Hit
TGTAAACATCCTTGACTGGAAGCT165250.21117762869183862No Hit
CTCCCACTGCTTCACTTGACTAG160910.20563142047082453No Hit
TATTGCACTTGTCCCGGCCTGTA156370.1998296266175056No Hit
TTCTCACTACTGCACTTGACTAG152560.19496072032209924No Hit
CACCCGGCTGTGTGCACATGTGC150640.19250709825197318No Hit
CTACGCCTGTCTGAGCGTCGCT146840.18765097123818203No Hit
GCCTGTCTGAGCGTCGCTT144060.18409833094914535No Hit
ATCCCGGACGAGCCCCCA140580.1796511409470419No Hit
CAACGGAATCCCAAAAGCAGCT134800.17226471617343325No Hit
CCCCCACTGCTAAATTTGACTGGCTT134480.17185577916174558No Hit
GCCTGTCTGAGCGTCGCG133820.17101234657513975No Hit
ACCTGTCTGAGCGTCGCT133400.1704756167472997No Hit
AACGGAATCCCAAAAGCAGCTG131010.16742136844125735No Hit
TGAGAACTGAATTCCATGGGTT127720.16321698478984342No Hit
TCCTTCTGATCGAGGCCC127520.16296139915753866No Hit
TTCACAGTGGCTAAGTTCCG112090.14324296762522354No Hit
ACGCCTGTCTGAGCGTCGCT102880.13147324925758763No Hit
TGAGGTAGTAGTTTGTACAGTT101690.1299525147453741No Hit
CGCGACCTCAGATCAGACGTGG100760.12876404155515678No Hit
NCCTGTCTGAGCGTCGCT97210.12422739658174663No Hit
TACGCCTGTCTGAGCGTCGCT97060.12403570735751804No Hit
GCCTGTCTGAGCGTCGCA91970.11753105301536096No Hit
TGTAAACATCCCCGACTGGAAGCT91560.1170071024691361No Hit
CTTCTCACTACTGCACTTGACTAG87860.11227876827149737No Hit
CCCCCCACTGCTAAATTTGACTGG87690.1120615204840383No Hit
TAGCAGCACGTAAATATTGGC87450.11175481772527254No Hit
AGCTCGGTCTGAGGCCCCTCAGT87190.1114225564032763No Hit
CCACCCTGAACGCGCCCGA82740.10573577608449457No Hit
TGTAAACATCCTACACTCTCAGCT81040.10356329820990379No Hit
TGAGGTAGTAGGTTGTGTGGTT80620.10302656838206371No Hit
AACATTCAACGCTGTCGGTGAGTT80140.1024131628645322No Hit
TTCAAGTAATCCAGGATAGGC78930.1008668697890882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)