FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8254101
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT85063310.30558022006273No Hit
CAACGGAATCCCAAAAGCAGCTG6009777.280950402714965No Hit
CCCCCCACTGCTAAATTTGACTG4750005.754715140994762No Hit
TTCAAGTAATCCAGGATAGGCT2851763.4549613579964675No Hit
TAGCAGCACGTAAATATTGGCG2534193.0702192764542136No Hit
AAGCTGCCAGTTGAAGAACTGT2315412.80516315465488No Hit
CCCCCACTGCTAAATTTGACTG1859152.2523955061853496No Hit
AACATTCAACGCTGTCGGTGAGT1331241.6128225230100772No Hit
TAGCTTATCAGACTGATGTTGAC1089611.3200831925851162No Hit
AACATTCAACGCTGTCGGTGAG1083251.3123779318910684No Hit
TAGCTTATCAGACTGATGTTGA1058041.2818355384795994No Hit
TGAGGTAGTAGGTTGTATAGTT1011721.2257179794625725No Hit
CATAAAGTAGAAAGCACTACT964481.1684858229866584No Hit
TAGCTTATCAGACTGATGTTGACT915621.1092910057679206No Hit
TATTGCACTTGTCCCGGCCTGTAA695760.8429264434733716No Hit
TATTGCACTTGTCCCGGCCTGT667770.8090160273056992No Hit
TGAGGTAGTAGATTGTATAGTT638610.7736881339348767No Hit
TATTGCACTTGTCCCGGCCTGTAT591870.7170617369474883No Hit
TCCTGTCTGAGCGTCGCT585050.7087991775239969No Hit
TGTAAACATCCTTGACTGGAAGCT526530.6379010869869415No Hit
TTCTCACTACTGCACTTGACTAG506140.6131982150448607No Hit
TTCAAGTAATTCAGGATAGGTT466490.5651614876047676No Hit
AACCGGGCGGAAACACCA453500.5494238560928683No Hit
TCGTACGACTCTTAGCGG429110.5198749082425814No Hit
CCCATAAAGTAGAAAGCACTAC402800.48799984395635576No Hit
TATTGCACTTGTCCCGGCCTGTA383800.4649809833923767No Hit
CTCCCACTGCTTCACTTGACTAG377460.45729995307786997No Hit
CAACGGAATCCCAAAAGCAGCTGT354350.4293017495182092No Hit
CGCCTGTCTGAGCGTCGCT330280.40014048774057887No Hit
CCCCCACTGCTAAATTTGACTGGCTT315630.382391734726774No Hit
AGCAGCATTGTACAGGGCTATGA304280.3686409943372391No Hit
ACCGGGTGCTGTAGGCTT295020.357422328609742No Hit
CTTCTCACTACTGCACTTGACTAG263600.31935640235078294No Hit
CATTGCACTTGTCTCGGTCTGA261080.3163033745286131No Hit
CAACACCAGTCGATGGGCTGT258310.3129474669621804No Hit
ACTGGACTTGGAGTCAGAAGGC244120.2957560126778192No Hit
TGAGGTAGTAGTTTGTACAGTT241630.2927393304249609No Hit
CCCATAAAGTAGAAAGCACT239440.29008610386521805No Hit
TGTAAACATCCCCGACTGGAAGCT231640.28063625584421614No Hit
CTACGCCTGTCTGAGCGTCGCT216020.26171232942267125No Hit
ACCGGGTGCTGTAGGCTTA190720.23106089930326756No Hit
ACCGGGTGCTGTAGGCTTT189100.22909823856044406No Hit
TTCACAGTGGCTAAGTTCCG184550.22358582721485962No Hit
TGATGTAGTAGGTTGTATAGTT173200.20983508682532476No Hit
TAGCAGCACGTAAATATTGGC170700.20680628938269593No Hit
GGAATACCGGGTGCTGTAGGCTTT167470.2028930830868195No Hit
CACCCGGCTGTGTGCACATGTGC161470.19562396922451036No Hit
TTCAAGTAATCCAGGATAGGC157050.19026905534594257No Hit
AACGGAATCCCAAAAGCAGCTG152320.18453857058448886No Hit
CCCCCCACTGCTAAATTTGACTGGCT150670.18253956427235382No Hit
CCCCCCACTGCTAAATTTGACTGG147140.17826290228336192No Hit
CAACGGAATCCCAAAAGCAGCT140160.16980649982354226No Hit
GGAATACCGGGTGCTGTAGGCTT139020.16842536818970352No Hit
TGTAAACATCCTACACTCTCAGCT138670.1680013365477355No Hit
CTCCCACTGCTTCACTTGACTAGCCT138370.16763788085462003No Hit
TGAGGTAGTAGTTTGTGCTGTT134990.16354294671218586No Hit
TTCACAGTGGCTAAGTTCCGC133300.16149547964096878No Hit
TCCCACTGCTTCACTTGACTAG132370.16036876699231084No Hit
TAGCTTATCAGACTGATGTTGACA131180.15892705940961951No Hit
AGAATTACTCTGAGACCT129330.1566857493020742No Hit
AGCTCGGTCTGAGGCCCCTCAGT126130.1528088885755093No Hit
TACGCCTGTCTGAGCGTCGCT124460.15078565188383328No Hit
TTCACAGTGGCTAAGTTCTGC118560.14363768991922926No Hit
ACCCCCCACTGCTAAATTTGACTG114720.13898545704735138No Hit
ACGCCTGTCTGAGCGTCGCT112880.13675626212957656No Hit
ATCCCGGACGAGCCCCCA111720.13535090011619677No Hit
CAAAGAATTCTCCTTTTGGGCT110410.1337638102562593No Hit
TGATGTAGTAGATTGTATAGTT109640.1328309406439296No Hit
TATTGCACTTGTCCCGGCCTGTT108950.13199499254976405No Hit
TGTCAGTTTGTCAAATACCCCA107930.1307592431931715No Hit
AACATTCAACGCTGTCGGTGAGTT102380.1240353128705355No Hit
TTCAAGTAATCCAGGATAGGCTT102150.12375666350581366No Hit
TTCACAGTGGCTAAGTTCTG99350.12036441037006937No Hit
TGTCAGTTTGTCAAATACCCCAA99260.12025537366213473No Hit
TTATAAAGCAATGAGACTGATT97710.11837751924770486No Hit
GTCAGGATGGCCGAGCGGTCTAAGG96620.11705696356271869No Hit
CCCATAAAGTAGAAAGCACTA95930.11622101546855314No Hit
TGAGGTAGTAGGTTGTATAGT95490.11568794711865048No Hit
TGAGGTAGTAGGTTGTGTGGTT94810.11486411421425544No Hit
TAGTGGGTTATCAGAACT89390.10829768135863616No Hit
GACCCGCGGGCGCTCTCCAGTCCT83790.10151317508714759No Hit
TGTAAACATCCCCGACTGGAAGC83640.10133144724058987No Hit
CACTAGATTGTGAGCTCCTGGA83450.10110125863495008No Hit
TAGCAGCACGTAAATATTGGCGT83440.10108914344517955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)