FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9943299
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9251949.304698571369522No Hit
CAACGGAATCCCAAAAGCAGCTG4583464.609596875242311No Hit
AACATTCAACGCTGTCGGTGAGT3048113.0654916441716176No Hit
AAGCTGCCAGTTGAAGAACTGT2916582.9332116031107986No Hit
CCCCCACTGCTAAATTTGACTG2904392.9209520904480493No Hit
TAGCAGCACGTAAATATTGGCG2652802.667927415237136No Hit
TTCAAGTAATCCAGGATAGGCT2649192.6642968294526796No Hit
TAGCTTATCAGACTGATGTTGAC1842491.8529966764551684No Hit
AACATTCAACGCTGTCGGTGAG1465071.4734244640536305No Hit
TAGCTTATCAGACTGATGTTGA1453151.4614364910478908No Hit
TAGCTTATCAGACTGATGTTGACT1428851.4369979219170619No Hit
TGAGGTAGTAGGTTGTATAGTT1033351.0392426095202407No Hit
TATTGCACTTGTCCCGGCCTGTAA984970.9905867257939241No Hit
TATTGCACTTGTCCCGGCCTGTAT828580.8333049222395907No Hit
TATTGCACTTGTCCCGGCCTGT818630.8232981830275846No Hit
TGTAAACATCCTTGACTGGAAGCT751790.7560770323813052No Hit
CATAAAGTAGAAAGCACTACT740050.7442700858135716No Hit
CGCCTGTCTGAGCGTCGCT721090.7252019676769249No Hit
AGCAGCATTGTACAGGGCTATGA657720.6614706044744304No Hit
TGAGGTAGTAGATTGTATAGTT569840.5730894746301002No Hit
TATTGCACTTGTCCCGGCCTGTA504700.5075780181205453No Hit
ACCGGGTGCTGTAGGCTT485410.48817801818088746No Hit
ACTGGACTTGGAGTCAGAAGGC465060.46771197366186007No Hit
TTCAAGTAATTCAGGATAGGTT457300.45990772277892883No Hit
TGTAAACATCCCCGACTGGAAGCT406510.4088280961881967No Hit
CGCGACCTCAGATCAGACGTGG401720.40401078153236664No Hit
ACCGGGTGCTGTAGGCTTT398970.40124509984060625No Hit
ACCGGGTGCTGTAGGCTTA351240.3532429226959785No Hit
CGCGACCTCAGATCAGACGTGGCG339720.3416572306635856No Hit
CCCATAAAGTAGAAAGCACTAC318950.3207687911225439No Hit
TTCTCACTACTGCACTTGACTAG315330.3171281483137538No Hit
TCGTACGACTCTTAGCGG313090.31487537486301076No Hit
CAACGGAATCCCAAAAGCAGCTGT291950.29361482542162315No Hit
CATTGCACTTGTCTCGGTCTGA285570.28719844389673893No Hit
GCCTGTCTGAGCGTCGCT270140.27168045534987934No Hit
AACATTCAACGCTGTCGGTGAGTT253320.25476454042063906No Hit
TACGCCTGTCTGAGCGTCGCT238930.24029248240448167No Hit
CAACACCAGTCGATGGGCTGT226320.22761057471971827No Hit
CCCATAAAGTAGAAAGCACT222780.22405038810559755No Hit
TGAGGTAGTAGTTTGTACAGTT222630.2238995327405924No Hit
CTACGCCTGTCTGAGCGTCGCT222550.22381907654592303No Hit
CTTCTCACTACTGCACTTGACTAG220740.22199875514152798No Hit
TGTAAACATCCTACACTCTCAGCT218040.21928335857143588No Hit
CACCCGGCTGTGTGCACATGTGC217870.21911238915776343No Hit
TGATGTAGTAGGTTGTATAGTT204530.20569631869664182No Hit
TAGCTTATCAGACTGATGTTGACA194300.19540798280329297No Hit
CCCCCACTGCTAAATTTGACTGGCTT192630.19372845973956934No Hit
CAACGGAATCCCAAAAGCAGCT190680.19176733999450282No Hit
CCCCCCACTGCTAAATTTGACTGG181710.18274618916719693No Hit
ACGCCTGTCTGAGCGTCGCT179210.18023193308377833No Hit
TTCAAGTAATCCAGGATAGGCTT174670.17566604403629016No Hit
GCGTTCGCGCTTTCCCCTG162250.1631752198138666No Hit
GACCCGCGGGCGCTCTCCAGTCCT160560.16147558270147563No Hit
TTATAAAGCAATGAGACTGATT159830.1607414199251174No Hit
CGCGACCTCAGATCAGACGTGGC153710.1545865210329087No Hit
AGCTCGGTCTGAGGCCCCTCAGT148670.1495177807687368No Hit
ACCCCCCACTGCTAAATTTGACTG145130.1459575941546161No Hit
CCCCCCACTGCTAAATTTGACTGGCT143790.14460995289390371No Hit
TAGCAGCACGTAAATATTGGC139560.14035583160075948No Hit
AGCAGCATTGTACAGGGCTATGT139010.13980269526240738No Hit
TGAGGTAGTAGTTTGTGCTGTT137030.13781140444433987No Hit
CTCCCACTGCTTCACTTGACTAG134490.13525692026358657No Hit
TATTGCACTTGTCCCGGCCTGTT129530.13026863619408408No Hit
CAAAGAATTCTCCTTTTGGGCT126570.12729175699131645No Hit
ACCACAGGGTAGAACCACGGAC121720.12241410018948438No Hit
TGAGGTAGTAGGTTGTGTGGTT117830.11850191772368507No Hit
GGAATACCGGGTGCTGTAGGCTTT117780.11845163260201669No Hit
CAAAGAATTCTCCTTTTGGGCTT117750.11842146152901567No Hit
TTCAAGTAATCCAGGATAGGC113210.11385557248152751No Hit
CGCGACCTCAGATCAGACGTG112610.11325215102150706No Hit
TGATGTAGTAGATTGTATAGTT111700.11233696180714269No Hit
TTCACAGTGGCTAAGTTCCG110950.1115826849821171No Hit
TGTAAACATCCCCGACTGGAAGC109240.10986293382105879No Hit
AACGGAATCCCAAAAGCAGCTG109210.10983276274805776No Hit
TATTGCACTTGTCCCGGCCTGTAG107940.10855552065768112No Hit
ACTTGACTTGGAGTCAGAAGGC105500.10610160672026457No Hit
AGCAGCATTGTACAGGGCTATG103990.10458299604587974No Hit
CACTAGATTGTGAGCTCCTGGA103250.10383877624518784No Hit
TGTAAACATCCTACACTCAGCT101430.1020083978164591No Hit
TAGCAGCACGTAAATATTGGCGT101310.10188771352445503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)