FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6890402
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5425917.874591351854362No Hit
CAACGGAATCCCAAAAGCAGCTG3555875.160613270459401No Hit
TAGCAGCACGTAAATATTGGCG2021992.934502224979036No Hit
AAGCTGCCAGTTGAAGAACTGT1988082.8852888409123296No Hit
TTCAAGTAATCCAGGATAGGCT1782972.5876139011918315No Hit
AACATTCAACGCTGTCGGTGAGT1753252.544481439544456No Hit
CCCCCACTGCTAAATTTGACTG1490942.1637924753882287No Hit
TAGCTTATCAGACTGATGTTGAC939061.3628522689967872No Hit
AACATTCAACGCTGTCGGTGAG804871.1681031092235257No Hit
TAGCTTATCAGACTGATGTTGA650440.9439797561883907No Hit
TAGCTTATCAGACTGATGTTGACT610770.886406917912772No Hit
TATTGCACTTGTCCCGGCCTGTAA562620.8165271053851431No Hit
TATTGCACTTGTCCCGGCCTGTAT507640.7367349539257652No Hit
TATTGCACTTGTCCCGGCCTGT468000.6792056544741512No Hit
CATAAAGTAGAAAGCACTACT452850.6572185483517508No Hit
TGTAAACATCCTTGACTGGAAGCT448670.6511521388737551No Hit
TGAGGTAGTAGGTTGTATAGTT422180.612707357277558No Hit
GGAATACCGGGTGCTGTAGGCTTT372220.5402007023683089No Hit
TTCTCACTACTGCACTTGACTAG364200.5285613234177048No Hit
AGCAGCATTGTACAGGGCTATGA363070.526921361046859No Hit
CGCCTGTCTGAGCGTCGCT297430.4316584141244589No Hit
TTCAAGTAATTCAGGATAGGTT290930.42222500225676235No Hit
CCCATAAAGTAGAAAGCACTAC283880.41199337861564533No Hit
TATTGCACTTGTCCCGGCCTGTA281020.4078426773938589No Hit
GGAATACCGGGTGCTGTAGGCTT266480.38674086069288843No Hit
TGTAAACATCCCCGACTGGAAGCT265400.38517346302871736No Hit
CAACGGAATCCCAAAAGCAGCTGT263160.3819225641696958No Hit
CCCCCACTGCTAAATTTGACTGGCTT252660.36668397576803213No Hit
TGAGGTAGTAGATTGTATAGTT249790.36251876160491076No Hit
ACCGGGTGCTGTAGGCTT241530.35053107206226863No Hit
CTTCTCACTACTGCACTTGACTAG232090.3368308554421063No Hit
ACTGGACTTGGAGTCAGAAGGC221470.32141811174442364No Hit
CTACGCCTGTCTGAGCGTCGCT218410.31697715169593876No Hit
TGAATACCGGGTGCTGTAGGCTTT212040.30773240806559615No Hit
TGTAAACATCCTACACTCTCAGCT208140.30207236094497825No Hit
ACCGGGTGCTGTAGGCTTT203840.2958317961709636No Hit
TTAATGCTAATCGTGATAGGGGTT191040.27725523126226886No Hit
CCCCCCACTGCTAAATTTGACTGGCT180710.2622633628632988No Hit
AACCGGGCGGAAACACCA173420.25168342863014376No Hit
ACCGGGTGCTGTAGGCTTA171440.24880986624583007No Hit
CCCCCCACTGCTAAATTTGACTGG169430.2458927650375116No Hit
CGCGACCTCAGATCAGACGTGG166000.2409148261596348No Hit
CGCGACCTCAGATCAGACGTGGCG162170.23535636962836132No Hit
AACATTCAACGCTGTCGGTGAGTT154230.2238330942084366No Hit
TGAATACCGGGTGCTGTAGGCTT153340.22254144242962892No Hit
CATTGCACTTGTCTCGGTCTGA151480.21984203534133423No Hit
AGCTCGGTCTGAGGCCCCTCAGT137610.1997125857098033No Hit
TGATGTAGTAGGTTGTATAGTT135580.19676645861881498No Hit
CTCCCACTGCTTCACTTGACTAG135080.1960408115520691No Hit
TGTCAGTTTGTCAAATACCCCAA133230.1933559174051093No Hit
CACCCGGCTGTGTGCACATGTGC129870.18847956911657696No Hit
ACCCCCCACTGCTAAATTTGACTG129200.18750720204713744No Hit
TACGCCTGTCTGAGCGTCGCT127580.18515610555088077No Hit
CAACGGAATCCCAAAAGCAGCT123670.17948154548892795No Hit
ACGCCTGTCTGAGCGTCGCT122410.1776529148807283No Hit
GACCCGCGGGCGCTCTCCAGTCCT118880.17252984658950232No Hit
GGGCTACGCCTGTCTGAGCGTCGCT116340.16884355949043323No Hit
CCCATAAAGTAGAAAGCACT114320.16591194534077983No Hit
TGAGGTAGTAGTTTGTACAGTT112220.1628642276604471No Hit
TAGCTTATCAGACTGATGTTGACA110690.16064374763620468No Hit
CAAAGAATTCTCCTTTTGGGCT108360.15726223230516884No Hit
TGTAAACATCCTACACTCAGCT103310.14993319693103538No Hit
TGTCAGTTTGTCAAATACCCCA101720.14762563925878344No Hit
TGTAAACATCCCCGACTGGAAGC95250.13823576621509168No Hit
CGCGACCTCAGATCAGACGTGGC93160.13520256147609386No Hit
TCGTACGACTCTTAGCGG93060.13505743206274468No Hit
TTCAAGTAATCCAGGATAGGCTT92660.13447691440934795No Hit
TAGCAGCACGTAAATATTGGC91780.1331997755718752No Hit
TTCAAGTAATCCAGGATAGGC86600.1256820719603878No Hit
CAAAGAATTCTCCTTTTGGGCTT85690.12436139429891027No Hit
TTCACAGTGGCTAAGTTCCG83570.12128465073590772No Hit
GTCAGGATGGCCGAGCGGTCTAAGG83350.12096536602653953No Hit
TGATGTAGTAGATTGTATAGTT82620.11990592130909053No Hit
TAGCAGCACGTAAATATTGGCGT78900.11450710713250113No Hit
ACTTGACTTGGAGTCAGAAGGC78760.11430392595381228No Hit
TGAGGTAGTAGTTTGTGCTGTT76980.11172062239619691No Hit
TATTGCACTTGTCCCGGCCTGTT76860.11154646710017789No Hit
CTCCCACTGCTTCACTTGACTAGCCT75000.1088470600118832No Hit
CCCCCACTGCTAAATTTGACTGGCT74190.10767151176375486No Hit
CAACACCAGTCGATGGGCTGT72350.10500113055812998No Hit
GTGCGGTAACGCGACCGATCCCGGA71250.10340470701128902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)