FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7881998
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG118655615.054000267444877No Hit
CAACGGAATCCCAAAAGCAGCTG4204385.334155121582117No Hit
CCCCCACTGCTAAATTTGACTG3202304.062802350368523No Hit
TTCAAGTAATCCAGGATAGGCT1624702.061279386267289No Hit
AACATTCAACGCTGTCGGTGAGT1620582.0560522852200673No Hit
TATTGCACTTGTCCCGGCCTGTAT1434251.819652834217923No Hit
TAGCAGCACGTAAATATTGGCG1410251.7892037019040095No Hit
TATTGCACTTGTCCCGGCCTGTAA1297311.6459151600901192No Hit
AAGCTGCCAGTTGAAGAACTGT1282901.627632993563307No Hit
AACATTCAACGCTGTCGGTGAG1221451.549670527701225No Hit
GCCTGTCTGAGCGTCGCT1050441.3327077728261285No Hit
TAGCTTATCAGACTGATGTTGACT1023511.298541308942225No Hit
TAGCTTATCAGACTGATGTTGAC899861.141664841833251No Hit
TAGCTTATCAGACTGATGTTGA748620.9497845597017406No Hit
TATTGCACTTGTCCCGGCCTGT742280.9417409139154818No Hit
TATTGCACTTGTCCCGGCCTGTA630300.7996703373941481No Hit
TGAGGTAGTAGGTTGTATAGTT564400.7160620949155277No Hit
TGTAAACATCCTTGACTGGAAGCT435810.5529181814052732No Hit
ACCGGGTGCTGTAGGCTTA432800.5490993527275698No Hit
TGAGGTAGTAGATTGTATAGTT383360.4863741401609084No Hit
TCCTGTCTGAGCGTCGCT364500.4624461970175582No Hit
AGCAGCATTGTACAGGGCTATGA355930.45157331935379835No Hit
TTCAAGTAATTCAGGATAGGTT350190.44429090187538745No Hit
TGTAAACATCCCCGACTGGAAGCT335430.42556468550233073No Hit
GGAATACCGGGTGCTGTAGGCTTT328470.4167344371312959No Hit
ACCGGGTGCTGTAGGCTT318460.4040346115287012No Hit
ACCGGGTGCTGTAGGCTTT310400.393808777926612No Hit
CATAAAGTAGAAAGCACTACT299190.3795864957083217No Hit
CACCCGGCTGTGTGCACATGTGC254860.32334441089683097No Hit
CCCCCACTGCTAAATTTGACTGGCTT238670.30280393372340364No Hit
GGAATACCGGGTGCTGTAGGCTT232680.29520433778338945No Hit
ACCCCCCACTGCTAAATTTGACTG230640.29261616153670683No Hit
CAACGGAATCCCAAAAGCAGCTGT225870.28656439648931653No Hit
ACTGGACTTGGAGTCAGAAGGC219360.27830506934916754No Hit
TTCTCACTACTGCACTTGACTAG215420.2733063367943001No Hit
TGATGTAGTAGGTTGTATAGTT213250.2705532277475838No Hit
CCCATAAAGTAGAAAGCACTAC204970.26004827709928374No Hit
CATTGCACTTGTCTCGGTCTGA204050.2588810603605837No Hit
GACCCGCGGGCGCTCTCCAGTCCT196950.249873192051051No Hit
TATTGCACTTGTCCCGGCCTGTAG190180.24128399931083463No Hit
CACTAGATTGTGAGCTCCTGGA178970.2270617170925443No Hit
CACTAGATTGTGAGCTCCTGGAA170960.21689931918277575No Hit
TCGTACGACTCTTAGCGG170700.21656945358270835No Hit
TTAATGCTAATCGTGATAGGGGTT163450.2073712781962137No Hit
CTTCTCACTACTGCACTTGACTAG159490.202347171364418No Hit
ACCTGGTGCTGTAGGCTTA155870.19775442724040276No Hit
TATTGCACTTGTCCCGGCCTGTT152960.19406246994734075No Hit
CAAAGAATTCTCCTTTTGGGCT150690.19118248951598313No Hit
CCCCCCACTGCTAAATTTGACTGG148140.18794726920762986No Hit
TGATGTAGTAGATTGTATAGTT146010.18524490871477003No Hit
CTCCCACTGCTTCACTTGACTAG145250.1842806861914961No Hit
AGCTCGGTCTGAGGCCCCTCAGT142740.18109621443699936No Hit
CCCATAAAGTAGAAAGCACT142270.1804999189291852No Hit
CCCCCCACTGCTAAATTTGACTGGCT139220.1766303416976254No Hit
CGCCCCACTGCTAAATTTGACTG136690.17342049566620038No Hit
AACCGGGCGGAAACACCA133920.1699061583116362No Hit
TATTGCACTTGTCCCGGCCTGTTT130970.1661634524647177No Hit
TAGCTTATCAGACTGATGTTGACA129800.16467905726441442No Hit
TGAATACCGGGTGCTGTAGGCTTT127370.1615960826176307No Hit
TGTAAACATCCTACACTCTCAGCT122830.15583612175491543No Hit
TGAGGTAGTAGTTTGTACAGTT122060.15485921209317738No Hit
CAACGGAATCCCAAAAGCAGCT119370.15144637184632628No Hit
CTCCCCACTGCTAAATTTGACTG114590.14538191966047187No Hit
ACCTGGTGCTGTAGGCTT113380.1438467759063121No Hit
ACCTGGTGCTGTAGGCTTT112820.14313629615232076No Hit
TATTGCACTTGTCCCGGCCTGTAAT112080.1421974479059751No Hit
AACATTCAACGCTGTCGGTGAGTT107390.1362471799662979No Hit
CGCGACCTCAGATCAGACGTGG105510.13386199793504133No Hit
CAACACCAGTCGATGGGCTGT104300.13232685418088155No Hit
CCCGCCACTGCTAAATTTGACTG103310.1310708274729326No Hit
ATCCCGGACGAGCCCCCA101300.1285207126416424No Hit
GGGAATACCGGGTGCTGTAGGCTTT98120.12448620261004888No Hit
CCCCCCACTGCTAAATTTGACTGA97150.1232555501790282No Hit
CTCCCACTGCTTCACTTGACTAGCCT94960.12047706685538362No Hit
GGGAATACCGGGTGCTGTAGGCTT94750.12021063694763688No Hit
CAAAGAATTCTCCTTTTGGGCTT94150.11944940863978905No Hit
CCCCCCACTGCTAAATTTGACTGT93260.11832025331648144No Hit
ACTTGACTTGGAGTCAGAAGGC93190.11823144334723251No Hit
TAGCAGCACGTAAATATTGGC91000.11545296002358793No Hit
TTCAAGTAATCCAGGATAGGCTT90850.11526265294662597No Hit
TGTAAACATCCCCGACTGGAAGC90170.1143999275310651No Hit
TGAATACCGGGTGCTGTAGGCTT89450.11348645356164769No Hit
CGCGACCTCAGATCAGACGTGGCG88790.11264910242301507No Hit
TATTGCACTTGTCCCGGCCTGTAAA88680.11250954389990964No Hit
CCCCCCACTGCTAAATTTTACTG87700.11126620433042486No Hit
ATACCGGGTGCTGTAGGCTTA80540.10218221319010737No Hit
TGAGAACTGAATTCCATGGGTT80060.10157323054382911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)