FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9545803
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTT100116210.48798094827643No Hit
GGAATACCGGGTGCTGTAGGCTT7338557.687724123366049No Hit
CCCCCCACTGCTAAATTTGACTG4729084.954093437712888No Hit
TGAATACCGGGTGCTGTAGGCTTT3678843.8538821720917564No Hit
TGAATACCGGGTGCTGTAGGCTT2700482.828971014800955No Hit
CAACGGAATCCCAAAAGCAGCTG2514432.6340686058574643No Hit
AAGCTGCCAGTTGAAGAACTGT1624841.7021511967091716No Hit
TTCAAGTAATCCAGGATAGGCT1576621.6516368502471714No Hit
GGAATACCGGGTGCTGTAGGCTTA1549651.6233835959112084No Hit
AACATTCAACGCTGTCGGTGAGT1490671.561597279977389No Hit
TAGCAGCACGTAAATATTGGCG1360511.4252441622773904No Hit
CCCCCACTGCTAAATTTGACTG1340351.4041249332298185No Hit
GGAATACCGGGTGCTGTAGGCT972761.0190447047775866No Hit
TAGCTTATCAGACTGATGTTGAC766890.8033792442605405No Hit
AACATTCAACGCTGTCGGTGAG666680.6984011716981797No Hit
TAGCTTATCAGACTGATGTTGA634690.6648890617164424No Hit
TGAATACCGGGTGCTGTAGGCTTA591840.6200002241822925No Hit
TAGCTTATCAGACTGATGTTGACT515110.5396193489431952No Hit
TATTGCACTTGTCCCGGCCTGTAA497120.5207733702444938No Hit
TATTGCACTTGTCCCGGCCTGT492920.516373530859583No Hit
TATTGCACTTGTCCCGGCCTGTAT470880.49328484989686044No Hit
ACCGGGTGCTGTAGGCTT464250.48633938915353686No Hit
TGAGGTAGTAGGTTGTATAGTT456770.4785034847251719No Hit
CTACGCCTGTCTGAGCGTCGCT422790.4429066889396314No Hit
ACCGGGTGCTGTAGGCTTT397550.41646574939792913No Hit
GCCTGTCTGAGCGTCGCT366110.3835298088594537No Hit
TGAATACCGGGTGCTGTAGGCT362190.3794232921002036No Hit
CATAAAGTAGAAAGCACTACT361610.37881569523276354No Hit
TGTAAACATCCTTGACTGGAAGCT356460.37342065408221814No Hit
CGCCTGTCTGAGCGTCGCT341500.3577488452254881No Hit
ACCGGGTGCTGTAGGCTTA331670.3474511259031849No Hit
GGGCTACGCCTGTCTGAGCGTCGCT330490.34621498055218614No Hit
TATTGCACTTGTCCCGGCCTGTA296620.310733418655298No Hit
GGAATACCGGGTGCTGTAGGCTA292080.3059774017963706No Hit
TGAGGTAGTAGATTGTATAGTT281110.2944854403553059No Hit
TTCTCACTACTGCACTTGACTAG264650.27724226028967913No Hit
TTCAAGTAATTCAGGATAGGTT256200.2683902024795609No Hit
AGCAGCATTGTACAGGGCTATGA252870.2649017583958102No Hit
TACGCCTGTCTGAGCGTCGCT232610.24367777126764506No Hit
AACCGGGCGGAAACACCA212140.22223379217023437No Hit
TTAATGCTAATCGTGATAGGGGTT204380.21410456511620868No Hit
TGTAAACATCCCCGACTGGAAGCT203400.21307793592639612No Hit
GAATACCGGGTGCTGTAGGCTTT197680.20708577371646997No Hit
ACTGGACTTGGAGTCAGAAGGC197520.20691816078752095No Hit
CCCATAAAGTAGAAAGCACTAC193530.2027383133718557No Hit
ACGCCTGTCTGAGCGTCGCT185520.19434719111634716No Hit
ATACCGGGTGCTGTAGGCTTT185230.19404339268262713No Hit
CGAATACCGGGTGCTGTAGGCTTT184830.19362436036025465No Hit
ATACCGGGTGCTGTAGGCTT176470.18486658482267024No Hit
CTTCTCACTACTGCACTTGACTAG176410.18480372997431435No Hit
GACCCGCGGGCGCTCTCCAGTCCT165660.1735422363105545No Hit
TCGTACGACTCTTAGCGG163990.17179277636464949No Hit
CAACGGAATCCCAAAAGCAGCTGT163160.1709232842957266No Hit
ATACCGGGTGCTGTAGGCTTA160980.16863955813879672No Hit
GAATACCGGGTGCTGTAGGCTT158540.16608346097232468No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT157740.16524539632757979No Hit
CATTGCACTTGTCTCGGTCTGA154730.16209217810172702No Hit
TGTAAACATCCTACACTCTCAGCT146950.15394199943158265No Hit
CGCGACCTCAGATCAGACGTGG146120.15307250736265982No Hit
TCCTGTCTGAGCGTCGCT144070.15092496671050093No Hit
ATCCCGGACGAGCCCCCA141040.14775079686852954No Hit
AATACCGGGTGCTGTAGGCTT139560.14620037727575147No Hit
CGAATACCGGGTGCTGTAGGCTT139360.14599086111456522No Hit
CCCCCACTGCTAAATTTGACTGGCTT137490.14403188500747396No Hit
AACATTCAACGCTGTCGGTGAGTT131650.13791401310083604No Hit
AGCTCGGTCTGAGGCCCCTCAGT124060.1299628747838186No Hit
CTCCCACTGCTTCACTTGACTAG115220.12070226045938723No Hit
TGATGTAGTAGGTTGTATAGTT113940.11936135702779536No Hit
CACCCGGCTGTGTGCACATGTGC112980.11835567945410144No Hit
CGGCCCCGCCGGGGTCGGC112300.11764332450606826No Hit
TTCACAGTGGCTAAGTTCCG112110.11744428415294136No Hit
TGGCTACGCCTGTCTGAGCGTCGCT109680.11489866279452865No Hit
TGAGGTAGTAGTTTGTACAGTT108690.11386155779665683No Hit
TGAATACCGGGTGCTGTAGGCTA107120.11221685593134491No Hit
CGCGACCTCAGATCAGACGTGGCG107030.1121225736588111No Hit
AATACCGGGTGCTGTAGGCTTT105930.11097023477228685No Hit
CCCCCCACTGCTAAATTTGACTGGCT104290.10925220225055975No Hit
CCCCCCACTGCTAAATTTGACTGG104280.10924172644250042No Hit
CCCATAAAGTAGAAAGCACT101920.10676943574050293No Hit
ACCCCCCACTGCTAAATTTGACTG100320.10509330645101307No Hit
AATACCGGGTGCTGTAGGCTTA96650.10124868489324575No Hit
GGGAATACCGGGTGCTGTAGGCTTT95530.10007539439060287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)