FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6657503
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG90544613.60038440838855No Hit
CAACGGAATCCCAAAAGCAGCTG3970365.963737455319209No Hit
CCCCCACTGCTAAATTTGACTG3549275.331233046383907No Hit
AACATTCAACGCTGTCGGTGAGT1871082.810483149613301No Hit
TTCAAGTAATCCAGGATAGGCT1529202.296957282632843No Hit
AACATTCAACGCTGTCGGTGAG1432452.1516325264892857No Hit
TAGCAGCACGTAAATATTGGCG1235871.8563566550401855No Hit
AAGCTGCCAGTTGAAGAACTGT1033511.5523988498390464No Hit
TAGCTTATCAGACTGATGTTGA1001701.504618172909573No Hit
TAGCTTATCAGACTGATGTTGAC976461.4667060608158944No Hit
TAGCTTATCAGACTGATGTTGACT953591.4323538419734847No Hit
TATTGCACTTGTCCCGGCCTGTAA923931.3878026040694236No Hit
TATTGCACTTGTCCCGGCCTGTAT764141.1477876915714496No Hit
TGAGGTAGTAGGTTGTATAGTT756251.1359364013805175No Hit
TATTGCACTTGTCCCGGCCTGT490360.7365524281401No Hit
TGAGGTAGTAGATTGTATAGTT447770.6725794941436751No Hit
TATTGCACTTGTCCCGGCCTGTA426600.6407807852283356No Hit
ACCGGGTGCTGTAGGCTT395030.593360603817978No Hit
GGAATACCGGGTGCTGTAGGCTTT394650.5927898192460447No Hit
CATAAAGTAGAAAGCACTACT344280.517130822171616No Hit
ACCGGGTGCTGTAGGCTTA335440.5038525705508506No Hit
TTCAAGTAATTCAGGATAGGTT325190.48845640775528004No Hit
ACCGGGTGCTGTAGGCTTT323950.4865938475731817No Hit
TGTAAACATCCTTGACTGGAAGCT307970.4625908542587214No Hit
AGCAGCATTGTACAGGGCTATGA291320.43758147762006266No Hit
GGAATACCGGGTGCTGTAGGCTT281470.422786140689685No Hit
GCCTGTCTGAGCGTCGCT264270.39695062848638596No Hit
CACCCGGCTGTGTGCACATGTGC260740.39164834022605777No Hit
TCGTACGACTCTTAGCGG253290.3804579584868381No Hit
TGTAAACATCCCCGACTGGAAGCT244690.3675402023851886No Hit
ACTGGACTTGGAGTCAGAAGGC238490.35822740147469706No Hit
CATTGCACTTGTCTCGGTCTGA232080.34859916698497917No Hit
TTCTCACTACTGCACTTGACTAG230840.34673660680288093No Hit
CCCCCACTGCTAAATTTGACTGGCTT214130.32163710628444325No Hit
CAACGGAATCCCAAAAGCAGCT187910.28225297082104206No Hit
CAACGGAATCCCAAAAGCAGCTGT184840.2776416323056858No Hit
CAACACCAGTCGATGGGCTGT163530.24563263433752866No Hit
CCCATAAAGTAGAAAGCACTAC159050.23890338464736702No Hit
GACCCGCGGGCGCTCTCCAGTCCT154230.23166343297179137No Hit
CCCATAAAGTAGAAAGCACT151390.22739756932892108No Hit
CTTCTCACTACTGCACTTGACTAG149540.22461874970240345No Hit
ACCCCCCACTGCTAAATTTGACTG148610.2232218295658297No Hit
TGAATACCGGGTGCTGTAGGCTTT145660.21879073881003133No Hit
TGATGTAGTAGGTTGTATAGTT142950.21472014357334876No Hit
TGAGGTAGTAGTTTGTACAGTT141550.21261725304517323No Hit
TAGCTTATCAGACTGATGTTGACA124990.187743062226183No Hit
AACATTCAACGCTGTCGGTGAGTT122940.18466382966706887No Hit
CACTAGATTGTGAGCTCCTGGA115190.17302282852895448No Hit
CTCCCACTGCTTCACTTGACTAG114500.17198640391149653No Hit
CCCCCCACTGCTAAATTTGACTGG109990.16521209228144546No Hit
TATTGCACTTGTCCCGGCCTGTAG108810.1634396559791261No Hit
CACTAGATTGTGAGCTCCTGGAA103900.15606451848388203No Hit
CGCGACCTCAGATCAGACGTGG102610.15412685506863458No Hit
TGAATACCGGGTGCTGTAGGCTT101180.15197890260056962No Hit
CCCCCCACTGCTAAATTTGACTGGCT98120.14738258473184315No Hit
CGCGACCTCAGATCAGACGTGGCG96980.1456702310160431No Hit
AACGGAATCCCAAAAGCAGCTG95910.14406302182665182No Hit
CTCCCACTGCTTCACTTGACTAGCCT95410.14331198949516058No Hit
CTCCCCACTGCTAAATTTGACTG94910.14256095716366932No Hit
CAAAGAATTCTCCTTTTGGGCT94240.14155457383947104No Hit
CCCGCCACTGCTAAATTTGACTG93580.1405632111619026No Hit
TATTGCACTTGTCCCGGCCTGTT92290.13862554774665517No Hit
GGGAATACCGGGTGCTGTAGGCTT91860.13797965994157269No Hit
TGAGGTAGTAGTTTGTGCTGTT91540.13749899924941827No Hit
TTCAAGTAATCCAGGATAGGCTT89300.13413437440433748No Hit
TCCTGTCTGAGCGTCGCT89220.13401420923129886No Hit
CGCCCCACTGCTAAATTTGACTG88360.13272243362113392No Hit
GGGAATACCGGGTGCTGTAGGCTTT87660.13167098835704616No Hit
TAGCAGCACGTAAATATTGGC86330.12967324235527944No Hit
TGTAAACATCCTACACTCTCAGCT85450.12835142545185485No Hit
TGATGTAGTAGATTGTATAGTT85380.12824628092544607No Hit
TTATAAAGCAATGAGACTGATT81360.1222079809802564No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC78570.11801722057053522No Hit
AGCTCGGTCTGAGGCCCCTCAGT77570.11651515590755274No Hit
ACCTGGTGCTGTAGGCTT75850.11393160468722281No Hit
TTCAAGTAATCCAGGATAGGC75040.11271493231020699No Hit
TTCACAGTGGCTAAGTTCTG72550.10897479129938056No Hit
TCAGTGCACTACAGAACTTTGT72040.10820873832125949No Hit
CGCGACCTCAGATCAGACGTGGC68150.10236570678225755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)