FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7644901
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5002096.543040910536317No Hit
GCCTGTCTGAGCGTCGCT4618646.041464761937402No Hit
AAGCTGCCAGTTGAAGAACTGT3396444.442752103657065No Hit
TAGCAGCACGTAAATATTGGCG2567933.359010142838998No Hit
TTCAAGTAATCCAGGATAGGCT2406513.14786286964344No Hit
CCCCCCACTGCTAAATTTGACTG2262882.959986008975133No Hit
AACATTCAACGCTGTCGGTGAGT1940302.5380315585512485No Hit
CGCCTGTCTGAGCGTCGCT1636662.140851791278919No Hit
TCCTGTCTGAGCGTCGCT1607982.1033365899702297No Hit
CATAAAGTAGAAAGCACTACT976671.2775443396847128No Hit
AACATTCAACGCTGTCGGTGAG976431.277230404945728No Hit
TGAGGTAGTAGGTTGTATAGTT954631.2487146661546042No Hit
TAGCTTATCAGACTGATGTTGAC928841.2149797623278575No Hit
CCCCCACTGCTAAATTTGACTG858451.1229053195064267No Hit
TATTGCACTTGTCCCGGCCTGT823421.0770839282287632No Hit
TAGCTTATCAGACTGATGTTGA703030.919606414785489No Hit
TATTGCACTTGTCCCGGCCTGTAA615790.8054911371644969No Hit
TAGCTTATCAGACTGATGTTGACT592750.7753534022219516No Hit
TGAGGTAGTAGATTGTATAGTT566640.7411999187432251No Hit
TGTAAACATCCTTGACTGGAAGCT546340.7146462720707567No Hit
TATTGCACTTGTCCCGGCCTGTAT527910.6905387002395452No Hit
AGCAGCATTGTACAGGGCTATGA485090.6345275105589987No Hit
TTCAAGTAATTCAGGATAGGTT466790.6105899867114041No Hit
CCCATAAAGTAGAAAGCACTAC408000.5336890562742409No Hit
ACGCCTGTCTGAGCGTCGCT355120.46451876878457943No Hit
TATTGCACTTGTCCCGGCCTGTA346890.453753423360224No Hit
ACTGGACTTGGAGTCAGAAGGC336020.4395347958070353No Hit
CAACGGAATCCCAAAAGCAGCTGT319550.4179910243442001No Hit
ATCCCGGACGAGCCCCCA317240.414969402481471No Hit
CTACGCCTGTCTGAGCGTCGCT317130.4148255157261029No Hit
TACGCCTGTCTGAGCGTCGCT305050.39902413386386554No Hit
CATTGCACTTGTCTCGGTCTGA284870.37262745456088964No Hit
TGTAAACATCCCCGACTGGAAGCT284770.37249664841964597No Hit
CCCATAAAGTAGAAAGCACT262910.3439024259437761No Hit
TGATGTAGTAGGTTGTATAGTT262810.34377161980253246No Hit
TGAGGTAGTAGTTTGTACAGTT250840.3281141246956632No Hit
TTAATGCTAATCGTGATAGGGGTT239370.31311066029501233No Hit
TTCTCACTACTGCACTTGACTAG231130.30233223425653255No Hit
TGTAAACATCCTACACTCTCAGCT217320.28426790615077946No Hit
CACCCGGCTGTGTGCACATGTGC213240.278931015588037No Hit
GCGTTCGCGCTTTCCCCTG206950.27070330930380915No Hit
ACCGGGTGCTGTAGGCTT206850.2705725031625655No Hit
AACCGGGCGGAAACACCA198170.2592185301026135No Hit
TTCACAGTGGCTAAGTTCCG177090.23164459552844438No Hit
TGAGGTAGTAGTTTGTGCTGTT167150.21864246508882193No Hit
ATCCCACCGCTGCCACCA163320.21363258987918873No Hit
AGCTCGGTCTGAGGCCCCTCAGT161430.21116035380968307No Hit
AACATTCAACGCTGTCGGTGAGTT160200.20955143827238576No Hit
CTCCCACTGCTTCACTTGACTAG157860.20649057456728348No Hit
TGATGTAGTAGATTGTATAGTT155440.20332506594918626No Hit
ACCGGGTGCTGTAGGCTTT151360.19798817538644384No Hit
CAACGGAATCCCAAAAGCAGCT143940.1882823597061623No Hit
CTTCTCACTACTGCACTTGACTAG140470.1837433866050064No Hit
TACCTGGTTGATCCTGCC139520.1825007282631914No Hit
ACCGGGTGCTGTAGGCTTA137270.17955759008520844No Hit
TCGTACGACTCTTAGCGG133450.1745607954896996No Hit
TGTAAACATCCTACACTCAGCT131860.172480977843925No Hit
TTATAAAGCAATGAGACTGATT128000.16743186079191869No Hit
AACGGAATCCCAAAAGCAGCTG125110.16365156330997616No Hit
CAACACCAGTCGATGGGCTGT124690.16310217751675266No Hit
TAGCAGCACGTAAATATTGGC122910.16077382820261504No Hit
TTCACAGTGGCTAAGTTCCGC122140.15976662091503865No Hit
TTCAAGTAATCCAGGATAGGC122090.15970121784441682No Hit
TTCAAGTAATCCAGGATAGGCTT121560.1590079452958253No Hit
CCCTGTCTGAGCGTCGCT118290.15473058447715674No Hit
TGAGGTAGTAGGTTGTGTGGTT116580.15249379946188968No Hit
TTCACAGTGGCTAAGTTCTGC116510.15240223516301912No Hit
TATTGCACTTGTCCCGGCCTGTT107610.14076048859233103No Hit
TGAGGTAGTAGGTTGTATAGT106250.13898152507141687No Hit
CAAAGTGCTGTTCGTGCAGGT105140.13752957690361195No Hit
ACCACAGGGTAGAACCACGGAC104080.1361430318064289No Hit
GGAATACCGGGTGCTGTAGGCTTT102950.13466492241037523No Hit
TAGTGGGTTATCAGAACT101370.13259818537872498No Hit
TAGCAGCACGTAAATATTGGCGT99960.130753818787189No Hit
TGTAAACATCCCCGACTGGAAGC98580.12894869403802614No Hit
AGAGGTAGTAGGTTGCATAGTT97420.12743134279959936No Hit
TTCACCACCTTCTCCACCCAGC94710.12388649637189546No Hit
AGCAGCATTGTACAGGGCTATG94640.12379493207302489No Hit
TAGCAGCACATAATGGTTTGT91830.1201192795040773No Hit
CCTCGACACAAGGGTTTGT91830.1201192795040773No Hit
AGCAGCATTGTACAGGGCTATGT91150.11922979774362022No Hit
ACTTGACTTGGAGTCAGAAGGC89310.1168229647447364No Hit
AATGACACGATCACTCCCGTTG88760.11610353096789612No Hit
CAAAGAATTCTCCTTTTGGGCT84940.1111067363723873No Hit
CAAAGTGCTGTTCGTGCAGGTAG84860.11100209145939234No Hit
CCCCCCACTGCTAAATTTGACTGG83430.10913156363960763No Hit
TCGTTCGCGCTTTCCCCTG83360.10903999934073703No Hit
TAGCTTATCAGACTGATGTTGACA82380.10775809915654894No Hit
CCCCCCACTGCTAAATTTGACTGGCT81600.10673781125484817No Hit
TGTCAGTTTGTCAAATACCCCA80290.10502425080455588No Hit
GGAATACCGGGTGCTGTAGGCTT79450.10392547921810891No Hit
CACTAGATTGTGAGCTCCTGGA78630.10285286885991067No Hit
CAAAGAATTCTCCTTTTGGGCTT78490.10266974026216952No Hit
TGAGAACTGAATTCCATGGGTT78300.1024212085938065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)