FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6826566
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6732399.862044840700287No Hit
CCCCCCACTGCTAAATTTGACTG5808328.508406715763094No Hit
AACATTCAACGCTGTCGGTGAGT1881922.75675940143258No Hit
CCCCCACTGCTAAATTTGACTG1839132.694077813061501No Hit
TTCAAGTAATCCAGGATAGGCT1792562.6258590336634846No Hit
TAGCAGCACGTAAATATTGGCG1759632.5776210176536782No Hit
AAGCTGCCAGTTGAAGAACTGT1757772.574896368100741No Hit
TATTGCACTTGTCCCGGCCTGTAT1470182.15361574179463No Hit
AACATTCAACGCTGTCGGTGAG1403082.0553232767397254No Hit
TATTGCACTTGTCCCGGCCTGTAA1361741.9947657431276573No Hit
GCCTGTCTGAGCGTCGCT1225121.7946358388683272No Hit
TAGCTTATCAGACTGATGTTGACT961591.408599872908282No Hit
TAGCTTATCAGACTGATGTTGAC950431.3922519755906557No Hit
TATTGCACTTGTCCCGGCCTGT926461.3571391531261838No Hit
TGAGGTAGTAGGTTGTATAGTT829211.2146809977373692No Hit
TATTGCACTTGTCCCGGCCTGTA629250.9217665221430511No Hit
TAGCTTATCAGACTGATGTTGA627380.919027223936603No Hit
TGAGGTAGTAGATTGTATAGTT527090.7721158778806211No Hit
TGTAAACATCCTTGACTGGAAGCT498730.7305722965250757No Hit
TTCAAGTAATTCAGGATAGGTT498580.7303525667224194No Hit
CATAAAGTAGAAAGCACTACT462380.6773244410147063No Hit
AGCAGCATTGTACAGGGCTATGA452470.6628076253858821No Hit
TCCTGTCTGAGCGTCGCT431080.6314741555270981No Hit
TTAATGCTAATCGTGATAGGGGTT401770.588538952088063No Hit
CAACGGAATCCCAAAAGCAGCTGT370050.5420734231530172No Hit
CACCCGGCTGTGTGCACATGTGC360900.5286699051909848No Hit
TGTAAACATCCCCGACTGGAAGCT350730.5137722245708897No Hit
TCGTACGACTCTTAGCGG288510.422628302429069No Hit
CCCATAAAGTAGAAAGCACTAC284250.416387976033631No Hit
ACTGGACTTGGAGTCAGAAGGC283020.41458619165184957No Hit
CATTGCACTTGTCTCGGTCTGA268400.3931698602196184No Hit
TGATGTAGTAGGTTGTATAGTT225340.33009275820375866No Hit
ATCCCGGACGAGCCCCCA204900.3001509104284643No Hit
CCCATAAAGTAGAAAGCACT201400.295023881699818No Hit
CACTAGATTGTGAGCTCCTGGA200190.29325139462505745No Hit
GGAATACCGGGTGCTGTAGGCTTT191960.28119555278598346No Hit
TATTGCACTTGTCCCGGCCTGTAG189480.2775626867153998No Hit
CACTAGATTGTGAGCTCCTGGAA188480.276097821364358No Hit
TATTGCACTTGTCCCGGCCTGTT185520.2717618199252743No Hit
CTCCCACTGCTTCACTTGACTAG183420.2686856026880865No Hit
TGAGGTAGTAGTTTGTACAGTT179750.2633095468497631No Hit
CAACGGAATCCCAAAAGCAGCT174360.25541392260764784No Hit
TTCTCACTACTGCACTTGACTAG167490.24535029764599067No Hit
TGAGAACTGAATTCCATGGGTT163860.24003283642170894No Hit
AGCTCGGTCTGAGGCCCCTCAGT163800.23994494450064646No Hit
TGTAAACATCCTACACTCTCAGCT161480.23654645688622947No Hit
CCCCCACTGCTAAATTTGACTGGCTT154290.22601407501223894No Hit
ACCGGGTGCTGTAGGCTTA151280.22160483030560313No Hit
TATTGCACTTGTCCCGGCCTGTTT147170.21558423371282137No Hit
TGATGTAGTAGATTGTATAGTT144960.21234688128701898No Hit
CAACACCAGTCGATGGGCTGT144230.21127752958075846No Hit
AACATTCAACGCTGTCGGTGAGTT137880.20197563460164306No Hit
AACGGAATCCCAAAAGCAGCTG133490.19554487571056955No Hit
CAAAGAATTCTCCTTTTGGGCT131670.19287882077167348No Hit
CTTCTCACTACTGCACTTGACTAG129010.18898227893790231No Hit
ACCGGGTGCTGTAGGCTT125580.18395779078382893No Hit
TAGCTTATCAGACTGATGTTGACA124850.1828884390775684No Hit
TATTGCACTTGTCCCGGCCTGTAAT119230.17465589580471352No Hit
TAGCAGCACGTAAATATTGGC118090.17298594930452588No Hit
GGAATACCGGGTGCTGTAGGCTT116520.17068611070339026No Hit
TGAGGTAGTAGTTTGTGCTGTT113960.16693605540472325No Hit
ACCCCCCACTGCTAAATTTGACTG113120.16570556850984813No Hit
TGAGAACTGAATTCCATAGGCTGT107040.15679918717551403No Hit
CGCCTGTCTGAGCGTCGCT106530.15605210584648269No Hit
TATTGCACTTGTCCCGGCCTGTAAA106130.15546615970606598No Hit
CCCCCCACTGCTAAATTTGACTGGCT104990.15379621320587833No Hit
CCCCCCACTGCTAAATTTGACTGG104810.1535325374426908No Hit
TTCAAGTAATCCAGGATAGGCTT101420.1485666439026591No Hit
ACCGGGTGCTGTAGGCTTT100890.14779026526660696No Hit
AACCGGGCGGAAACACCA100850.1477316706525653No Hit
TGTAAACATCCCCGACTGGAAGC95650.14011437082714792No Hit
CAAAGAATTCTCCTTTTGGGCTT91820.13450393653265785No Hit
TTATAAAGCAATGAGACTGATT91670.1342842067300016No Hit
AGCAGCATTGTACAGGGCTATGT90860.1330976657956577No Hit
CCCCCCACTGCTAAATTTGACTGT88590.12977242144879284No Hit
TCGTTTCCCGGGCGGCGCACCA86970.12739933958010513No Hit
ACTTGACTTGGAGTCAGAAGGC86370.12652042036948005No Hit
CTCCCACTGCTTCACTTGACTAGCCT84450.12370787889547982No Hit
TATTGCACTTGTCCCGGCCTGA83180.12184749989965674No Hit
CAAAGTGCTGTTCGTGCAGGTAG81740.11973809379415654No Hit
GACCCGCGGGCGCTCTCCAGTCCT77760.11390792969701018No Hit
TTCAAGTAATCCAGGATAGGC77740.11387863238998934No Hit
TTCACAGTGGCTAAGTTCCGC76290.11175457763097875No Hit
TTCACCACCTTCTCCACCCAGC75900.11118328014407244No Hit
TGAATACCGGGTGCTGTAGGCTTT74030.10844398193762428No Hit
TGAGGTAGTAGGTTGTGTGGTT72640.10640781909967618No Hit
ACTGGACTTGGAGTCAGAAGGCA69640.10201322304655078No Hit
TGAGGTAGTAGGTTGTATAGT69280.10148587152017574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)