FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8082632
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7176348.878716734845778No Hit
CAACGGAATCCCAAAAGCAGCTG7119498.808380735384217No Hit
TAGCAGCACGTAAATATTGGCG3367784.166687287012449No Hit
TTCAAGTAATCCAGGATAGGCT3311494.097044131169154No Hit
AAGCTGCCAGTTGAAGAACTGT2917433.6095049236436845No Hit
AACATTCAACGCTGTCGGTGAGT1858212.2990159641067414No Hit
CCCCCACTGCTAAATTTGACTG1804642.232738048695029No Hit
TAGCTTATCAGACTGATGTTGAC1551541.919597477653319No Hit
TATTGCACTTGTCCCGGCCTGTAA1288001.5935403220139182No Hit
TAGCTTATCAGACTGATGTTGACT1202641.487931159058089No Hit
TATTGCACTTGTCCCGGCCTGTAT1099101.359829322923523No Hit
TGTAAACATCCTTGACTGGAAGCT1028811.2728650766235554No Hit
TGAGGTAGTAGGTTGTATAGTT975341.2067108832865334No Hit
AACATTCAACGCTGTCGGTGAG945311.1695571442569699No Hit
TAGCTTATCAGACTGATGTTGA892971.1048010103639507No Hit
TTAATGCTAATCGTGATAGGGGTT849041.0504499029524046No Hit
TATTGCACTTGTCCCGGCCTGT706260.8737995247092779No Hit
AGCAGCATTGTACAGGGCTATGA647310.8008653616792153No Hit
GCCTGTCTGAGCGTCGCT618200.7648498657368046No Hit
TATTGCACTTGTCCCGGCCTGTA543700.6726769200923659No Hit
CATAAAGTAGAAAGCACTACT535880.6630018538515672No Hit
CAACGGAATCCCAAAAGCAGCTGT530570.6564322116855005No Hit
TGAGGTAGTAGATTGTATAGTT527970.653215437743547No Hit
TGTAAACATCCCCGACTGGAAGCT517170.6398534536769706No Hit
TTCAAGTAATTCAGGATAGGTT499740.6182886960584126No Hit
ACTGGACTTGGAGTCAGAAGGC496260.6139831678591825No Hit
CCCCCACTGCTAAATTTGACTGGCTT393710.4871061802640526No Hit
TGTAAACATCCTACACTCTCAGCT390930.48366670658765615No Hit
CCCATAAAGTAGAAAGCACTAC390460.4830852128366107No Hit
TTCTCACTACTGCACTTGACTAG344830.4266308301553256No Hit
CTTCTCACTACTGCACTTGACTAG344440.4261483140640326No Hit
CAAAGAATTCTCCTTTTGGGCT338790.41915801684401816No Hit
CCCCCCACTGCTAAATTTGACTGGCT288470.3569010688597477No Hit
CATTGCACTTGTCTCGGTCTGA251490.31114864563919276No Hit
TAGCTTATCAGACTGATGTTGACA225040.2784241568835498No Hit
AACATTCAACGCTGTCGGTGAGTT218100.2698378449000276No Hit
CCCCCCACTGCTAAATTTGACTGG213970.2647281232153091No Hit
AGCTCGGTCTGAGGCCCCTCAGT201520.24932472491633914No Hit
CAAAGAATTCTCCTTTTGGGCTT200590.24817410962171726No Hit
CACCCGGCTGTGTGCACATGTGC197910.2448583580200113No Hit
CTCCCACTGCTTCACTTGACTAG189450.23439147050119316No Hit
TTCACAGTGGCTAAGTTCCG179080.22156149135578607No Hit
TGAGGTAGTAGTTTGTACAGTT173560.21473203283286932No Hit
TAGCAGCACGTAAATATTGGC170630.21110697604443698No Hit
CACTAGATTGTGAGCTCCTGGA169430.20962231114815075No Hit
GACCCGCGGGCGCTCTCCAGTCCT168110.20798917976223588No Hit
CACTAGATTGTGAGCTCCTGGAA167060.2066900979779854No Hit
TGAGGTAGTAGTTTGTGCTGTT166070.20546524943854924No Hit
ACCCCCCACTGCTAAATTTGACTG158650.19628507149651253No Hit
CTCCCACTGCTTCACTTGACTAGCCT158360.19592627747991No Hit
TTCAAGTAATCCAGGATAGGCTT157450.19480040660022627No Hit
TATTGCACTTGTCCCGGCCTGTT156840.1940457019446141No Hit
CAACGGAATCCCAAAAGCAGCT156740.19392197986992354No Hit
AACGGAATCCCAAAAGCAGCTG152110.1881936478117524No Hit
GGGCTACGCCTGTCTGAGCGTCGCT149760.18528617905652514No Hit
CGCCTGTCTGAGCGTCGCT149290.1847046853054797No Hit
TGTAAACATCCCCGACTGGAAGC144870.17923616960415864No Hit
CCCATAAAGTAGAAAGCACT139970.1731737879443231No Hit
CTACGCCTGTCTGAGCGTCGCT130680.16168000720557363No Hit
TTCAAGTAATCCAGGATAGGC128920.15950249869102043No Hit
TAGCAGCACGTAAATATTGGCGT128210.15862407196071776No Hit
TATTGCACTTGTCCCGGCCTGTAG121680.1505450204834267No Hit
TTAATGCTAATCGTGATAGGGGT119990.14845411742115688No Hit
ACTGGACTTGGAGTCAGAAGGCA119800.14821904547924486No Hit
TGTAAACATCCTACACTCAGCT118910.14711791901449925No Hit
ATCACATTGCCAGGGATTTCCA117530.14541055438377004No Hit
TATTGCACTTGTCCCGGCCTGTAAT115400.14277527419286193No Hit
GTCAGGATGGCCGAGCGGTCTAAGG112890.13966985011812982No Hit
TTAATGCTAATCGTGATAGGGGTTT112540.139236822856713No Hit
TGAGAACTGAATTCCATGGGTT111930.13848211820110085No Hit
TATTGCACTTGTCCCGGCCTGTTT111930.13848211820110085No Hit
AGCAGCATTGTACAGGGCTATGT107960.1335703518358871No Hit
GTGCGGTAACGCGACCGATCCCGGA106100.13126912124664342No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT105500.1305267887985003No Hit
TACGCCTGTCTGAGCGTCGCT100420.12424170740422179No Hit
TATTGCACTTGTCCCGGCCTGTAAA99600.1232271863917595No Hit
CCCCCACTGCTAAATTTGACTGGCT95530.1181916979518553No Hit
CAAAGTGCTGTTCGTGCAGGTAG95360.11798137042488141No Hit
ACTGGACTTGGAGTCAGAAGG91820.11360160898083695No Hit
CCCATAAAGTAGAAAGCACTA91640.113378909246394No Hit
TGAGGTAGTAGGTTGTGTGGTT91220.1128592765326938No Hit
AGCAGCATTGTACAGGGCTATG90970.1125499713459675No Hit
GGAATACCGGGTGCTGTAGGCTTT89810.11111479527955745No Hit
ACTGGACTTGGAGTCAGAAGGCT89780.11107767865715029No Hit
CCCCCCACTGCTAAATTTGACTGT88910.11000129660734277No Hit
ACCGGGTGCTGTAGGCTT88620.10964250259074024No Hit
TGTCAGTTTGTCAAATACCCCA88120.10902389221728763No Hit
CCCCCCACTGCTAAATTTGACTGTA87780.10860323716333986No Hit
TTATAAAGCAATGAGACTGATT83580.10340691002633795No Hit
ATCACATTGCCAGGGATTTCC82840.10249136667362808No Hit
TGTAAACATCCTTGACTGGAAGC81600.10095721294746562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)