FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8036449
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG87063710.833603249395349No Hit
CCCCCCACTGCTAAATTTGACTG7309139.09497465858366No Hit
CCCCCACTGCTAAATTTGACTG3104223.8626761645597454No Hit
AACATTCAACGCTGTCGGTGAGT2900243.608857593695922No Hit
AACATTCAACGCTGTCGGTGAG2190032.7251215057794806No Hit
TTCAAGTAATCCAGGATAGGCT2018732.511967661338982No Hit
TAGCAGCACGTAAATATTGGCG2011742.5032697899283627No Hit
TATTGCACTTGTCCCGGCCTGTAA1543541.9206741684044781No Hit
AAGCTGCCAGTTGAAGAACTGT1509541.8783669254915945No Hit
TAGCTTATCAGACTGATGTTGACT1357251.6888678071620935No Hit
TAGCTTATCAGACTGATGTTGAC1292571.6083844991737022No Hit
TATTGCACTTGTCCCGGCCTGTAT1185721.4754277666665963No Hit
TAGCTTATCAGACTGATGTTGA987991.2293862625147003No Hit
TGAGGTAGTAGGTTGTATAGTT779050.9693958115082918No Hit
TATTGCACTTGTCCCGGCCTGT706480.8790947345027635No Hit
TATTGCACTTGTCCCGGCCTGTA608490.7571627717664854No Hit
CATAAAGTAGAAAGCACTACT523420.651307561337103No Hit
CACCCGGCTGTGTGCACATGTGC475620.5918285551242843No Hit
AGCAGCATTGTACAGGGCTATGA424910.5287285466503925No Hit
TGTAAACATCCTTGACTGGAAGCT421650.5246720286534513No Hit
TGAGGTAGTAGATTGTATAGTT407900.5075624818872115No Hit
TTCAAGTAATTCAGGATAGGTT404460.503281984368967No Hit
CAACGGAATCCCAAAAGCAGCTGT398260.4955671341907352No Hit
CAACGGAATCCCAAAAGCAGCT386690.4811702282936157No Hit
GCCTGTCTGAGCGTCGCT367610.45742839903544463No Hit
TGTAAACATCCCCGACTGGAAGCT357200.4444749167200588No Hit
CATTGCACTTGTCTCGGTCTGA351180.4369840460631306No Hit
ACTGGACTTGGAGTCAGAAGGC345030.4293314124185943No Hit
CCCCCACTGCTAAATTTGACTGGCTT293580.3653105992460103No Hit
TCGTACGACTCTTAGCGG263110.3273958436120232No Hit
CCCATAAAGTAGAAAGCACTAC253230.3151018565538088No Hit
TGATGTAGTAGGTTGTATAGTT251230.31261319520599207No Hit
CCCATAAAGTAGAAAGCACT234330.2915840068169412No Hit
AACATTCAACGCTGTCGGTGAGTT225160.2801734945372017No Hit
TTCTCACTACTGCACTTGACTAG223810.27849364812742544No Hit
CAACACCAGTCGATGGGCTGT222990.2774732969748206No Hit
CTTCTCACTACTGCACTTGACTAG204470.2544282928940381No Hit
CACTAGATTGTGAGCTCCTGGA184860.2300269683786956No Hit
TCCTGTCTGAGCGTCGCT181990.2264557393445787No Hit
TAGCTTATCAGACTGATGTTGACA180920.22512430552349677No Hit
CTCCCACTGCTTCACTTGACTAG176660.21982345685264723No Hit
CGCCCCACTGCTAAATTTGACTG175950.2189399820741723No Hit
CACTAGATTGTGAGCTCCTGGAA173850.2163268876589648No Hit
TAGCAGCACGTAAATATTGGC170610.2122952562755018No Hit
ACTTGACTTGGAGTCAGAAGGC157570.19606918428773704No Hit
TATTGCACTTGTCCCGGCCTGTAG156990.1953474724968702No Hit
AACGGAATCCCAAAAGCAGCTG155110.19300813082992252No Hit
CTCCCACTGCTTCACTTGACTAGCCT152450.18969821123732633No Hit
CAAAGAATTCTCCTTTTGGGCT149510.1860398790560358No Hit
TATTGCACTTGTCCCGGCCTGTT141100.17557505808846668No Hit
CGACGGAATCCCAAAAGCAGCTG140010.17421873765390658No Hit
TTAGGTAGTAGGTTGTATAGTT135990.16921652834479506No Hit
TTATGTAGTAGGTTGTATAGTT135560.16868146615501448No Hit
CCCCCCACTGCTAAATTTGACTGGCT132610.1650106906669849No Hit
TGATGTAGTAGATTGTATAGTT131870.16408988596829271No Hit
ACCCCCCACTGCTAAATTTGACTG131080.16310686473590513No Hit
TTTAAACATCCTTGACTGGAAGCT127560.15872682076374778No Hit
TGAGGTAGTAGTTTGTACAGTT127410.15854017116266153No Hit
TATTGCACTTGTCCCGGCCTGTAAA123740.1539734775894179No Hit
CAACGGAATCCCAAAAGCAGCTGA120750.15025292887443198No Hit
TATTGCACTTGTCCCGGCCTGTAAT119440.14862285569161204No Hit
CCCCCCACTGCTAAATTTGACTGG118760.1477767108333544No Hit
TTCAAGTAATCCAGGATAGGCTT118100.1469554525885749No Hit
TTCACAGTGGCTAAGTTCTG117460.14615908095727353No Hit
CAAGGGAATCCCAAAAGCAGCTG112430.1399000976675146No Hit
CCCGCCACTGCTAAATTTGACTG112160.13956412838555934No Hit
ATCAGCATTGTACAGGGCTATGA109950.13681415759622192No Hit
TTATAAAGCAATGAGACTGATT109120.135781363136878No Hit
CAAAGAATTCTCCTTTTGGGCTT108860.13545783716166182No Hit
TGTAAACATCCTACACTCTCAGCT108830.13542050724144458No Hit
CAATGGAATCCCAAAAGCAGCTG108320.1347858985977513No Hit
TTTAAACATCCCCGACTGGAAGCT107090.13325537186884406No Hit
ACTGGACTTGGAGTCAGAAGGCT106470.1324838868510209No Hit
ACCGGGTGCTGTAGGCTT101770.12663553268365169No Hit
CCCTCCACTGCTAAATTTGACTG97100.12082450843649975No Hit
ATGCACCTGGGCAAGGATTCTGA95230.11849761007629116No Hit
TGAGGTAGTAGTTTGTGCTGTT94790.1179501045797715No Hit
CGCCTGTCTGAGCGTCGCT92600.11522502040391222No Hit
CCCATAAAGTAGAAAGCACTA90970.11319676140544163No Hit
TTCAAGTAATCCAGGATAGGC87970.10946376938371662No Hit
TGCAAGTAATCCAGGATAGGCT87930.10941399615676027No Hit
TTCACAGTGGCTAAGTTCTGC87670.10909047018154412No Hit
AGCAGCATTGTACAGGGCTATGT87080.10835631508393818No Hit
ACCACAGGGTAGAACCACGGAC86890.1081198922558956No Hit
TATGGCACTTGTCCCGGCCTGTAA86330.10742306707850695No Hit
TTCACAGTGGCTAAGTTCCG86230.1072986340111161No Hit
ACTGGACTTGGAGTCAGAAGGCA85720.10666402536742285No Hit
TTAATGCTAATCGTGATAGGGGTT82680.1028812601187415No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC82340.10245818768961265No Hit
TATTGCACTTGTCCCGGCCTGTTT81980.10201022864700567No Hit
TTCACAGTGGCTAAGTTCCGC81080.10089033104048815No Hit
TGTAAACATCCCCGACTGGAAGC80980.10076589797309732No Hit
AGCTCGGTCTGAGGCCCCTCAGT80570.10025572239679491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)