FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8424022
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG110817213.154903916442764No Hit
CAACGGAATCCCAAAAGCAGCTG4096444.862807813179975No Hit
CCCCCACTGCTAAATTTGACTG3744214.444682124524366No Hit
AAGCTGCCAGTTGAAGAACTGT2908313.4524007653351334No Hit
TAGCAGCACGTAAATATTGGCG2638053.131580140697638No Hit
TTCAAGTAATCCAGGATAGGCT2513202.983373025379089No Hit
AACATTCAACGCTGTCGGTGAGT2471042.9333256727012347No Hit
TAGCTTATCAGACTGATGTTGAC1791062.126134048557803No Hit
GCCTGTCTGAGCGTCGCT1413771.6782600995106614No Hit
TAGCTTATCAGACTGATGTTGACT1371271.6278091391499216No Hit
TAGCTTATCAGACTGATGTTGA1207041.4328547575018205No Hit
AACATTCAACGCTGTCGGTGAG1118641.3279167599514816No Hit
CATAAAGTAGAAAGCACTACT789240.9368921401202419No Hit
TATTGCACTTGTCCCGGCCTGTAA751050.8915575006807912No Hit
TGTAAACATCCTTGACTGGAAGCT750080.890406031703146No Hit
TCCTGTCTGAGCGTCGCT748090.8880437396768432No Hit
TGAGGTAGTAGGTTGTATAGTT668620.793706379209361No Hit
TATTGCACTTGTCCCGGCCTGTAT662020.7858716418356932No Hit
TATTGCACTTGTCCCGGCCTGT651260.7730986457537742No Hit
TTCAAGTAATTCAGGATAGGTT536580.6369641484791944No Hit
AGCAGCATTGTACAGGGCTATGA434240.515478235930533No Hit
TGAGGTAGTAGATTGTATAGTT430730.511311580145446No Hit
TATTGCACTTGTCCCGGCCTGTA402680.4780139463073577No Hit
TGTAAACATCCCCGACTGGAAGCT342380.4064329366661198No Hit
CGCCTGTCTGAGCGTCGCT322960.38337981548481237No Hit
CCCATAAAGTAGAAAGCACTAC319680.3794861884263835No Hit
TTCTCACTACTGCACTTGACTAG310250.3682920106334005No Hit
ACTGGACTTGGAGTCAGAAGGC294250.3492987079093573No Hit
CGCCCCACTGCTAAATTTGACTG270300.3208681078943051No Hit
CAACGGAATCCCAAAAGCAGCTGT269020.31934864367638166No Hit
CTTCTCACTACTGCACTTGACTAG252330.29953625477236406No Hit
CATTGCACTTGTCTCGGTCTGA249440.2961055894678338No Hit
AACATTCAACGCTGTCGGTGAGTT235390.2794270955132833No Hit
TTTAAACATCCTTGACTGGAAGCT230570.2737053630676653No Hit
TGATGTAGTAGGTTGTATAGTT220180.26137158711123976No Hit
GGAATACCGGGTGCTGTAGGCTTT201430.23911381048150157No Hit
CCCATAAAGTAGAAAGCACT198730.2359086906468193No Hit
TGTAAACATCCTACACTCTCAGCT197170.2340568436312251No Hit
TAGCTTATCAGACTGATGTTGACA189550.22501128320889952No Hit
TGAGGTAGTAGTTTGTACAGTT187540.22262524955419158No Hit
CCCCCCACTGCTAAATTTGACTGG184190.21864852679634503No Hit
CCCGCCACTGCTAAATTTGACTG182650.21682042140915586No Hit
CAACACCAGTCGATGGGCTGT179920.213579689131866No Hit
TTATAAAGCAATGAGACTGATT171440.2035132386881231No Hit
TGTCAGTTTGTCAAATACCCCAA169420.2011153342192126No Hit
TTCAAGTAATCCAGGATAGGCTT164290.19502560653331627No Hit
AACCGGGCGGAAACACCA152580.18112488310215713No Hit
CCCTCCACTGCTAAATTTGACTG151940.18036515099319542No Hit
TCGTACGACTCTTAGCGG150840.17905936143091744No Hit
CAACGGAATCCCAAAAGCAGCT144280.17127210731405973No Hit
TGATGTAGTAGATTGTATAGTT140990.16736660944142834No Hit
CAAAGAATTCTCCTTTTGGGCTT138310.1641852312351511No Hit
ACTTGACTTGGAGTCAGAAGGC137180.16284382923026555No Hit
CTCCCACTGCTTCACTTGACTAG133940.15899768542864678No Hit
GGAATACCGGGTGCTGTAGGCTT133900.1589502021718367No Hit
CACCCGGCTGTGTGCACATGTGC133500.1584753696037356No Hit
GACCCGCGGGCGCTCTCCAGTCCT133470.15843975716112801No Hit
CAAAGAATTCTCCTTTTGGGCT131300.15586379047917967No Hit
TAGCAGCACGTAAATATTGGC127900.15182771365032047No Hit
CTACGCCTGTCTGAGCGTCGCT127010.15077121118629558No Hit
ACCCCCCACTGCTAAATTTGACTG123320.1463908807455631No Hit
TGCAAGTAATCCAGGATAGGCT121910.1447170959430068No Hit
TGAGGTAGTAGTTTGTGCTGTT121720.1444915504731588No Hit
TTAGGTAGTAGGTTGTATAGTT120840.1434469188233364No Hit
TTATGTAGTAGGTTGTATAGTT119200.14150010529412196No Hit
CCCCCACTGCTAAATTTGACTGGCTT118600.14078785644197034No Hit
TACGCCTGTCTGAGCGTCGCT116060.1377726696345285No Hit
TGTCAGTTTGTCAAATACCCCA115640.13727409543802235No Hit
TGAATACCGGGTGCTGTAGGCTTT114810.13628881785921262No Hit
ATCAGCATTGTACAGGGCTATGA113490.13472187038447905No Hit
TAGCAGCACGTAAATATTGGCGT111340.13216964533093575No Hit
TATTGCACTTGTCCCGGCCTGTT110420.13107753042430326No Hit
GCCCCCACTGCTAAATTTGACTG109690.1302109609875188No Hit
CCCCCCACTGCTAAATTTGACTGGCT107290.1273619655789123No Hit
TTCACAGTGGCTAAGTTCCG105830.12562882670534337No Hit
TTTAAACATCCCCGACTGGAAGCT102570.12175894127531955No Hit
ACGCCTGTCTGAGCGTCGCT102050.12114165893678815No Hit
CTCCCCACTGCTAAATTTGACTG101970.12104669242316793No Hit
TTCACAGTGGCTAAGTTCTGC101190.12012076891537082No Hit
ACCACAGGGTAGAACCACGGAC99200.11775847688906796No Hit
TTCAAGTAATCCAGGATAGGC96300.11431594077033512No Hit
TTCACAGTGGCTAAGTTCCGC94910.11266589759618387No Hit
TGTAAACATCCTACACTCAGCT88920.10555527988887019No Hit
TGTAAACATCCCCGACTGGAAGC85840.10189906911449187No Hit
CGCCCACTGCTAAATTTGACTG85770.10181597341507417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)