FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7209211
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6749599.362453117269006No Hit
CAACGGAATCCCAAAAGCAGCTG4014845.569042160092138No Hit
AAGCTGCCAGTTGAAGAACTGT2645153.669125511793177No Hit
CCCCCACTGCTAAATTTGACTG2345733.253795734373706No Hit
TTCAAGTAATCCAGGATAGGCT2004712.7807620001689504No Hit
GCCTGTCTGAGCGTCGCT1958492.7166495751060693No Hit
TAGCAGCACGTAAATATTGGCG1886212.616388950191637No Hit
AACATTCAACGCTGTCGGTGAGT1761952.4440261215825143No Hit
TAGCTTATCAGACTGATGTTGAC1140301.5817264885158722No Hit
AACATTCAACGCTGTCGGTGAG882421.2240174410209383No Hit
TAGCTTATCAGACTGATGTTGA796631.1050169012947464No Hit
TGAGGTAGTAGGTTGTATAGTT746241.0351202094098786No Hit
TATTGCACTTGTCCCGGCCTGT713520.9897338280152987No Hit
TAGCTTATCAGACTGATGTTGACT706430.979899187303576No Hit
TCCTGTCTGAGCGTCGCT690930.9583989149436742No Hit
CATAAAGTAGAAAGCACTACT666690.9247752632014793No Hit
CGCCTGTCTGAGCGTCGCT596180.8269698306791131No Hit
TATTGCACTTGTCCCGGCCTGTAA596010.8267340212403271No Hit
AGCAGCATTGTACAGGGCTATGA545910.7572395925157414No Hit
TATTGCACTTGTCCCGGCCTGTAT541500.7511224182507629No Hit
TGTAAACATCCTTGACTGGAAGCT523230.7257798391529947No Hit
TGAGGTAGTAGATTGTATAGTT461170.6396955228526395No Hit
TTCAAGTAATTCAGGATAGGTT434650.6029092504020204No Hit
TATTGCACTTGTCCCGGCCTGTA362610.50298153293058No Hit
CCCATAAAGTAGAAAGCACTAC333670.4628384437631247No Hit
TTCTCACTACTGCACTTGACTAG298110.41351265762647255No Hit
TGATGTAGTAGGTTGTATAGTT279120.38717135619972837No Hit
ACTGGACTTGGAGTCAGAAGGC265440.3681956319491828No Hit
TGTAAACATCCCCGACTGGAAGCT258330.35833324895054397No Hit
TTAATGCTAATCGTGATAGGGGTT251110.34831828337386717No Hit
CAACGGAATCCCAAAAGCAGCTGT245710.34082786590654646No Hit
CATTGCACTTGTCTCGGTCTGA235320.3264157478536833No Hit
TGAGGTAGTAGTTTGTACAGTT203510.2822916405137816No Hit
GGAATACCGGGTGCTGTAGGCTTT194390.26964115768008456No Hit
TGTAAACATCCTACACTCTCAGCT192920.2676020995917584No Hit
CTACGCCTGTCTGAGCGTCGCT188700.2617484770524819No Hit
GACCCGCGGGCGCTCTCCAGTCCT180900.250928985155241No Hit
CTTCTCACTACTGCACTTGACTAG176850.2453111720547505No Hit
TGATGTAGTAGATTGTATAGTT170580.23661396510658378No Hit
CAACACCAGTCGATGGGCTGT166410.23082969828459732No Hit
CCCATAAAGTAGAAAGCACT163840.227264814415891No Hit
ACGCCTGTCTGAGCGTCGCT161290.22372767283410072No Hit
AACATTCAACGCTGTCGGTGAGTT158770.22023214468268443No Hit
ACCGGGTGCTGTAGGCTT158600.2199963352438984No Hit
TGAGGTAGTAGTTTGTGCTGTT156190.21665338967052009No Hit
GGAATACCGGGTGCTGTAGGCTT148690.20625003207701925No Hit
TACGCCTGTCTGAGCGTCGCT147690.20486291773121912No Hit
AGCTCGGTCTGAGGCCCCTCAGT143790.1994531717825987No Hit
CTCCCACTGCTTCACTTGACTAG135520.18798173614283173No Hit
ACCGGGTGCTGTAGGCTTT135010.18727430782647367No Hit
AACCGGGCGGAAACACCA125540.17413833497174658No Hit
CCCCCCACTGCTAAATTTGACTGG123330.1710728122675283No Hit
TAGCTTATCAGACTGATGTTGACA122670.17015731679930024No Hit
TTCACAGTGGCTAAGTTCCG122330.1696856979217282No Hit
CACCCGGCTGTGTGCACATGTGC121290.16824309900209608No Hit
ATCCCGGACGAGCCCCCA120000.16645372149601392No Hit
ACTTGACTTGGAGTCAGAAGGC114540.15888007716794528No Hit
TTATAAAGCAATGAGACTGATT112900.1566052096408331No Hit
TTCAAGTAATCCAGGATAGGCTT109160.15141740198754064No Hit
TATTGCACTTGTCCCGGCCTGTT108780.1508902985361366No Hit
CCCCCACTGCTAAATTTGACTGGCTT106140.1472283166632243No Hit
TAGCAGCACGTAAATATTGGC103890.14410730938517405No Hit
ACCGGGTGCTGTAGGCTTA103210.14316407163002998No Hit
AGCAGCATTGTACAGGGCTATG101620.14095855982020777No Hit
TTCAAGTAATCCAGGATAGGC99700.13829530027627154No Hit
ACCCCCCACTGCTAAATTTGACTG97380.1350771949940153No Hit
TCGTACGACTCTTAGCGG95890.13301039461877312No Hit
CAAAGAATTCTCCTTTTGGGCT93970.1303471350748369No Hit
AGCAGCATTGTACAGGGCTATGT93190.12926518588511282No Hit
TGTCAGTTTGTCAAATACCCCA91610.12707354521874864No Hit
TGTAAACATCCTACACTCAGCT91600.12705967407529062No Hit
TAGCAGCACGTAAATATTGGCGT91150.12643547261968058No Hit
TTCACAGTGGCTAAGTTCCGC90520.1255615905818265No Hit
GCGTTCGCGCTTTCCCCTG90310.12527029656920846No Hit
CAAAGTGCTGTTCGTGCAGGTAG90160.12506222941733844No Hit
TTCACAGTGGCTAAGTTCTGC89700.1244241568182704No Hit
ACCACAGGGTAGAACCACGGAC87290.12108121124489211No Hit
CCCCCCACTGCTAAATTTGACTGGCT86300.11970796804255No Hit
AAGCTGCCAGTTGAAGCACTGT82970.11508887727103563No Hit
TGAGGTAGTAGGTTGTATAGT81030.11239787544018341No Hit
CAAAGAATTCTCCTTTTGGGCTT80800.11207883914064938No Hit
CAACGGAATCCCAAAAGCAGCT80260.11132979739391731No Hit
CGCCCCACTGCTAAATTTGACTG78820.10933235273596514No Hit
TGAATACCGGGTGCTGTAGGCTTT78810.10931848159250715No Hit
TGCAAGTAATCCAGGATAGGCT78100.10833363040698907No Hit
AACGGAATCCCAAAAGCAGCTG77220.10711296978268496No Hit
TGTCAGTTTGTCAAATACCCCAA77150.10701587177847896No Hit
TGTAAACATCCCCGACTGGAAGC76380.10594779373221286No Hit
TGATGTAGTAGTTTGTACAGTT76330.10587843801492286No Hit
TGAGGTAGTAGGTTGTGTGGTT75460.10467164853407676No Hit
CGCGACCTCAGATCAGACGTGG74750.10368679734855868No Hit
TCGTTTCCCGGCCAATGCACCA74510.10335388990556664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)