FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662270
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG121570118.247549258736136No Hit
CAACGGAATCCCAAAAGCAGCTG4968087.457037916505936No Hit
CCCCCACTGCTAAATTTGACTG4105866.162854402478435No Hit
GCCTGTCTGAGCGTCGCT1852182.780103478243902No Hit
TTCAAGTAATCCAGGATAGGCT1846472.77153282589868No Hit
AACATTCAACGCTGTCGGTGAGT1543732.317123142712619No Hit
TAGCAGCACGTAAATATTGGCG1369442.0555156125464746No Hit
AAGCTGCCAGTTGAAGAACTGT1341012.012842469608707No Hit
TATTGCACTTGTCCCGGCCTGTAT1299191.9500710718718994No Hit
AACATTCAACGCTGTCGGTGAG1158151.7383714559752157No Hit
TATTGCACTTGTCCCGGCCTGTAA1093611.6414975676458623No Hit
TGAGGTAGTAGGTTGTATAGTT984451.4776495098517473No Hit
TAGCTTATCAGACTGATGTTGACT836061.2549176181691826No Hit
TATTGCACTTGTCCCGGCCTGT826051.2398927092417449No Hit
TAGCTTATCAGACTGATGTTGAC804971.2082518420898583No Hit
TGAGGTAGTAGATTGTATAGTT668551.0034867995442995No Hit
TAGCTTATCAGACTGATGTTGA646900.9709903681477936No Hit
TATTGCACTTGTCCCGGCCTGTA627040.9411807086773727No Hit
TGTAAACATCCTTGACTGGAAGCT466010.6994763046228988No Hit
AGCAGCATTGTACAGGGCTATGA456770.6856071579206486No Hit
TTCAAGTAATTCAGGATAGGTT420000.6304157591931879No Hit
ACTGGACTTGGAGTCAGAAGGC345710.5189072193111357No Hit
GACCCGCGGGCGCTCTCCAGTCCT340360.5108769233309367No Hit
CATAAAGTAGAAAGCACTACT327400.4914240941901184No Hit
TGTAAACATCCCCGACTGGAAGCT322990.48480472871858993No Hit
CAACGGAATCCCAAAAGCAGCTGT259890.3900922658493276No Hit
ACCGGGTGCTGTAGGCTTA238120.3574157156644807No Hit
CATTGCACTTGTCTCGGTCTGA233170.34998581564541814No Hit
TTAATGCTAATCGTGATAGGGGTT228200.3425258958282988No Hit
TGAGGTAGTAGTTTGTACAGTT220330.3307131052929407No Hit
CCCATAAAGTAGAAAGCACTAC214540.32202237375549175No Hit
CACCCGGCTGTGTGCACATGTGC200400.30079837652932107No Hit
TCGTACGACTCTTAGCGG192640.28915069488327555No Hit
TTCTCACTACTGCACTTGACTAG181910.2730450732257924No Hit
ACCGGGTGCTGTAGGCTT181750.27280491484133784No Hit
ACCGGGTGCTGTAGGCTTT181450.2723546178704856No Hit
TATTGCACTTGTCCCGGCCTGTT173240.26003149076816157No Hit
ATCCCGGACGAGCCCCCA172670.25917592652354227No Hit
CCCATAAAGTAGAAAGCACT164120.24634246285425238No Hit
CACTAGATTGTGAGCTCCTGGA160800.2413591763768205No Hit
TATTGCACTTGTCCCGGCCTGTAG151240.2270097129056613No Hit
TGAGGTAGTAGTTTGTGCTGTT149660.2246381488591726No Hit
AGCTCGGTCTGAGGCCCCTCAGT144310.2166078528789737No Hit
GGAATACCGGGTGCTGTAGGCTTT143940.21605248661492252No Hit
ACCCCCCACTGCTAAATTTGACTG143250.2150168035819623No Hit
CACTAGATTGTGAGCTCCTGGAA142670.21414622943831457No Hit
AACGGAATCCCAAAAGCAGCTG136520.2049151415358429No Hit
CAACACCAGTCGATGGGCTGT136450.204810072242644No Hit
TGTAAACATCCTACACTCTCAGCT135680.20365431001745654No Hit
CTCCCACTGCTTCACTTGACTAG135240.2029938744602065No Hit
CAACGGAATCCCAAAAGCAGCT128030.19217173726072345No Hit
TATTGCACTTGTCCCGGCCTGTTT124920.18750365866288818No Hit
CCCCCACTGCTAAATTTGACTGGCTT119740.1797285309661722No Hit
CTTCTCACTACTGCACTTGACTAG119590.17950338248074604No Hit
TAGCTTATCAGACTGATGTTGACA116280.17453510590234259No Hit
CCCCCCACTGCTAAATTTGACTGG113360.1701522153860471No Hit
CAAAGAATTCTCCTTTTGGGCT111790.16779566123858686No Hit
AACATTCAACGCTGTCGGTGAGTT108760.16324766183297884No Hit
TGAGAACTGAATTCCATAGGCTGT107850.1618817610213936No Hit
TGAGAACTGAATTCCATGGGTT107350.16113126606997313No Hit
AACCGGGCGGAAACACCA106540.159915464248672No Hit
CCCCCCACTGCTAAATTTGACTGA106190.15939011778267767No Hit
ACTGGACTTGGAGTCAGAAGGCA103310.15506726686249583No Hit
GGAATACCGGGTGCTGTAGGCTT98000.1470970104784105No Hit
TTCAAGTAATCCAGGATAGGCTT94460.14178350622235364No Hit
TTATAAAGCAATGAGACTGATT93110.13975716985351838No Hit
TATTGCACTTGTCCCGGCCTGTAAT92780.13926184318558088No Hit
TAGCAGCACGTAAATATTGGC90860.13637994257212632No Hit
AGCAGCATTGTACAGGGCTATGT88250.1324623589257115No Hit
GACCCGCGGGCGCTCTCCAGTCCTT84940.12749408234730805No Hit
TGTAAACATCCCCGACTGGAAGC84570.1269387160832569No Hit
TGAGGTAGTAGGTTGTATAGT83770.12573792416098417No Hit
TCAGTGCACTACAGAACTTTGT83100.12473226092608075No Hit
TGAGGTAGTAGGTTGTGTGGTT82340.12359150859992164No Hit
TATTGCACTTGTCCCGGCCTGA76670.1150808958508136No Hit
TCGTTTCCCGGGCGGCGCACCA75860.11386509402951248No Hit
CAAAGAATTCTCCTTTTGGGCTT75400.11317463867420564No Hit
CCCCCCACTGCTAAATTTGACTGGCT74120.11125337159856925No Hit
TATTGCACTTGTCCCGGCCTGTAAA73980.11104323301217153No Hit
TTCAAGTAATCCAGGATAGGC73920.11095317361800108No Hit
TTCACCACCTTCTCCACCCAGC72500.10882176795596696No Hit
ACTGGACTTGGAGTCAGAAGGCT72270.10847654027831355No Hit
CCCCCCACTGCTAAATTTGACTGT72230.1084165006821999No Hit
CGCGACCTCAGATCAGACGTGGCG70110.10523440208817715No Hit
CGCCTGTCTGAGCGTCGCT69950.1049942437037226No Hit
TGAGGTAGTAAGTTGTATTGTT66950.10049127399519983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)