FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9194073
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG150516116.37099248613754No Hit
CAACGGAATCCCAAAAGCAGCTG6147316.68616618554149No Hit
CCCCCACTGCTAAATTTGACTG4330064.709621078710165No Hit
TAGCAGCACGTAAATATTGGCG2999343.262253845493722No Hit
AAGCTGCCAGTTGAAGAACTGT2918573.1744037707771082No Hit
TTCAAGTAATCCAGGATAGGCT2800813.0463212550085257No Hit
AACATTCAACGCTGTCGGTGAGT2022372.199645358482579No Hit
TAGCTTATCAGACTGATGTTGAC1897992.064362551830946No Hit
TAGCTTATCAGACTGATGTTGACT1374321.4947890885791313No Hit
AACATTCAACGCTGTCGGTGAG1093041.1888528620558048No Hit
TATTGCACTTGTCCCGGCCTGTAA972521.0577684123238962No Hit
TGTAAACATCCTTGACTGGAAGCT971611.056778644241785No Hit
TAGCTTATCAGACTGATGTTGA970851.0559520247446372No Hit
TATTGCACTTGTCCCGGCCTGTAT841440.9151983022105655No Hit
TTAATGCTAATCGTGATAGGGGTT806460.8771520521971057No Hit
TGAGGTAGTAGGTTGTATAGTT744740.8100218477708411No Hit
AGCAGCATTGTACAGGGCTATGA667340.725837177929738No Hit
TATTGCACTTGTCCCGGCCTGT571080.6211392926725728No Hit
TTCAAGTAATTCAGGATAGGTT524630.5706176141955801No Hit
CATAAAGTAGAAAGCACTACT502130.5461453264510734No Hit
TATTGCACTTGTCCCGGCCTGTA483130.5254798390223789No Hit
TGAGGTAGTAGATTGTATAGTT468380.5094368948343133No Hit
TGTAAACATCCCCGACTGGAAGCT460020.5003440803656878No Hit
TTCTCACTACTGCACTTGACTAG415300.4517040489019393No Hit
CAACGGAATCCCAAAAGCAGCTGT407520.4432420756285055No Hit
CCCATAAAGTAGAAAGCACTAC400510.4356175984245502No Hit
GACCCGCGGGCGCTCTCCAGTCCT360150.39171975249707064No Hit
ACTGGACTTGGAGTCAGAAGGC343230.3733165921132016No Hit
TGTAAACATCCTACACTCTCAGCT336840.36636646239376175No Hit
CCCCCACTGCTAAATTTGACTGGCTT331280.3603190881777858No Hit
CAAAGAATTCTCCTTTTGGGCT315060.34267728785707924No Hit
CTTCTCACTACTGCACTTGACTAG304550.3312460103373119No Hit
TAGCTTATCAGACTGATGTTGACA294620.3204455740127362No Hit
CCCCCCACTGCTAAATTTGACTGG245300.26680231927677756No Hit
AACATTCAACGCTGTCGGTGAGTT225030.2447555071620597No Hit
ACCCCCCACTGCTAAATTTGACTG219560.2388060220970619No Hit
CATTGCACTTGTCTCGGTCTGA201780.21946747649273612No Hit
TGAGGTAGTAGTTTGTACAGTT196300.2135071148554074No Hit
CCCCCCACTGCTAAATTTGACTGGCT191990.208819312180793No Hit
CAAAGAATTCTCCTTTTGGGCTT180150.19594145054101703No Hit
TTCACAGTGGCTAAGTTCCG179030.19472327443995713No Hit
CGCCCCACTGCTAAATTTGACTG177280.19281987428205105No Hit
TAGCAGCACGTAAATATTGGC173430.1886323939346577No Hit
CAACGGAATCCCAAAAGCAGCT160630.17471038135111608No Hit
AGCTCGGTCTGAGGCCCCTCAGT158330.17220876971501098No Hit
TGAGGTAGTAGTTTGTGCTGTT156940.170696926161017No Hit
TTTAAACATCCTTGACTGGAAGCT150550.16374679644157708No Hit
GCCTGTCTGAGCGTCGCT145870.1586565605907197No Hit
CTCCCACTGCTTCACTTGACTAG144250.1568945558731152No Hit
TTCAAGTAATCCAGGATAGGCTT138540.15068403307217595No Hit
TGTCAGTTTGTCAAATACCCCA135720.14761683967486444No Hit
TTAATGCTAATCGTGATAGGGGTTT132050.14362513762942714No Hit
CCCATAAAGTAGAAAGCACT131650.14319007473619147No Hit
CCCGCCACTGCTAAATTTGACTG131370.14288553071092647No Hit
CACCCGGCTGTGTGCACATGTGC130630.1420806643584405No Hit
TGATGTAGTAGGTTGTATAGTT127820.13902434753345988No Hit
ATCACATTGCCAGGGATTTCCA127580.13876330979751847No Hit
TATTGCACTTGTCCCGGCCTGTT123820.1346737186011031No Hit
CTACGCCTGTCTGAGCGTCGCT123630.13446706372681616No Hit
TAGCAGCACGTAAATATTGGCGT121090.13170441435476965No Hit
CTCCCACTGCTTCACTTGACTAGCCT120260.13080165885130562No Hit
CAAAGTGCTGTTCGTGCAGGTAG119050.1294855935992677No Hit
CGCCTGTCTGAGCGTCGCT118830.12924630900798809No Hit
TGTAAACATCCCCGACTGGAAGC118730.12913754328467916No Hit
TACCCGCGGGCGCTCTCCAGTCCT115010.1250914583775874No Hit
TATTGCACTTGTCCCGGCCTGTAG113310.12324244108133577No Hit
TTCAAGTAATCCAGGATAGGC112870.12276387189877652No Hit
TGTAAACATCCTACACTCAGCT111340.12109975633215006No Hit
ACTGGACTTGGAGTCAGAAGGCA110550.1202405071180096No Hit
ATCACATTGCCAGGGATTTCC110540.12022963054567873No Hit
AACGGAATCCCAAAAGCAGCTG106610.11595513761963822No Hit
AGCAGCATTGTACAGGGCTATGT102610.11160450868728147No Hit
TGTCAGTTTGTCAAATACCCCAA100870.1097119851017063No Hit
TTCACAGTGGCTAAGTTCTGC100150.10892887189388206No Hit
CAACACCAGTCGATGGGCTGT100130.10890711874922028No Hit
TCGTTTCCCGGCCAATGCACCA98740.10739527519522632No Hit
TAGCAGCACATCATGGTTTACA97650.10620972881115909No Hit
CACTAGATTGTGAGCTCCTGGAA94680.1029793868288842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)