FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667126
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT99482414.921331920230696No Hit
CAACGGAATCCCAAAAGCAGCTG5195957.79338803556435No Hit
TCCTGTCTGAGCGTCGCT3018214.527003089487135No Hit
AAGCTGCCAGTTGAAGAACTGT2137823.2065090715249718No Hit
CCCCCCACTGCTAAATTTGACTG1940262.9101894879442805No Hit
TTCAAGTAATCCAGGATAGGCT1876702.814856056417713No Hit
TAGCAGCACGTAAATATTGGCG1846552.769634172205535No Hit
AACATTCAACGCTGTCGGTGAGT1196121.7940563895147625No Hit
TGAGGTAGTAGGTTGTATAGTT1040831.5611374376305471No Hit
CCCCCACTGCTAAATTTGACTG938911.4082679703368437No Hit
AACATTCAACGCTGTCGGTGAG861871.2927159318722938No Hit
TAGCTTATCAGACTGATGTTGAC833031.2494589122809439No Hit
TCGTACGACTCTTAGCGG642670.9639385846315188No Hit
AACCGGGCGGAAACACCA582170.8731948368757393No Hit
CATAAAGTAGAAAGCACTACT565000.8474416112729832No Hit
TAGCTTATCAGACTGATGTTGACT526500.7896955899738508No Hit
CGCCTGTCTGAGCGTCGCT526110.7891106302775739No Hit
TAGCTTATCAGACTGATGTTGA525870.7887506550798651No Hit
TATTGCACTTGTCCCGGCCTGT518170.7772014508200386No Hit
TGAGGTAGTAGATTGTATAGTT492470.7386541067320461No Hit
TATTGCACTTGTCCCGGCCTGTAA403110.604623341451774No Hit
TGTAAACATCCTTGACTGGAAGCT400890.6012935708729669No Hit
ACCGGGTGCTGTAGGCTT387540.5812699505004105No Hit
TATTGCACTTGTCCCGGCCTGTAT337230.5058101496806869No Hit
ACTGGACTTGGAGTCAGAAGGC336840.5052251899844101No Hit
TTCAAGTAATTCAGGATAGGTT327380.4910361676080518No Hit
AGCAGCATTGTACAGGGCTATGA317880.4767871493654087No Hit
CAACGGAATCCCAAAAGCAGCTGT311870.46777277045611554No Hit
ACCGGGTGCTGTAGGCTTT285730.4285654718389903No Hit
CATTGCACTTGTCTCGGTCTGA259410.38908819182358334No Hit
TATTGCACTTGTCCCGGCCTGTA251030.376519057836915No Hit
CCCATAAAGTAGAAAGCACTAC236230.35432058731153426No Hit
CAACGGAATCCCAAAAGCAGCT228040.34203643368971876No Hit
CCCTGTCTGAGCGTCGCT223390.3350619142341093No Hit
ACCGGGTGCTGTAGGCTTA213950.3209028897908934No Hit
TTAATGCTAATCGTGATAGGGGTT205430.30812377027222826No Hit
CCCATAAAGTAGAAAGCACT198150.2972045226083923No Hit
TGTAAACATCCCCGACTGGAAGCT188750.28310549403146124No Hit
CAACACCAGTCGATGGGCTGT177410.26609666593971676No Hit
TGAGGTAGTAGTTTGTACAGTT175760.2636218364554682No Hit
CTACGCCTGTCTGAGCGTCGCT173690.26051705037522915No Hit
CACCCGGCTGTGTGCACATGTGC168720.2530625639893411No Hit
AACGGAATCCCAAAAGCAGCTG155990.2339688795441994No Hit
TACGCCTGTCTGAGCGTCGCT149480.2242045523063461No Hit
TGAGGTAGTAGTTTGTGCTGTT143660.21547515376190582No Hit
ACGCCTGTCTGAGCGTCGCT127300.1909368444514173No Hit
TAGCAGCACGTAAATATTGGC126680.1900069085240027No Hit
CTCCCACTGCTTCACTTGACTAG118930.17838270943132017No Hit
TTCTCACTACTGCACTTGACTAG117960.17692780967391347No Hit
TGTAAACATCCTACACTCTCAGCT116440.1746479667550906No Hit
CTCGACACAAGGGTTTGT106810.16020396194702186No Hit
TTCACAGTGGCTAAGTTCCGC106000.15898904565475439No Hit
ATCCCGGACGAGCCCCCA104410.15660420996993307No Hit
TTCACAGTGGCTAAGTTCTGC99970.14994466881231883No Hit
TTCACAGTGGCTAAGTTCCG99260.1488797421857634No Hit
TGAGGTAGTAGGTTGTGTGGTT98930.1483847762889137No Hit
GCCTGTCTGAGCGTCGCTT97740.1465998992669405No Hit
AGCTCGGTCTGAGGCCCCTCAGT97290.14592494577123635No Hit
TGAGGTAGTAGGTTGTATAGT89450.13416575597941302No Hit
TTATAAAGCAATGAGACTGATT87710.13155593579602365No Hit
TGAGAACTGAATTCCATGGGTT86430.12963606807490963No Hit
AACATTCAACGCTGTCGGTGAGTT85900.12884112284663587No Hit
TTCAAGTAATCCAGGATAGGC84360.12653128199467056No Hit
CCCCCACTGCTAAATTTGACTGGCTT83330.12498638843783663No Hit
AGAGGTAGTAGGTTGCATAGTT83170.12474640497269739No Hit
TGTCAGTTTGTCAAATACCCCAA83010.12450642150755811No Hit
TGAGAACTGAATTCCATAGGCTGT81620.12242156515416087No Hit
TAGTGGGTTATCAGAACT81590.12237656825444727No Hit
TTCAAGTAATCCAGGATAGGCTT80950.12141663439389025No Hit
TAGCTTATCAGACTGATGTTGACA79560.11933177804049301No Hit
AATGACACGATCACTCCCGTTG78310.1174569072190926No Hit
ACCACAGGGTAGAACCACGGAC78110.11715692788766853No Hit
AATGCACCCGGGCAAGGATTCT76070.11409713870714308No Hit
ACCTGTCTGAGCGTCGCT72010.10800755827923457No Hit
CAAAGTGCTGTTCGTGCAGGT71980.10796256137952095No Hit
TATTGCACTTGTCCCGGCCTGTT71960.10793256344637854No Hit
CACTAGATTGTGAGCTCCTGGA71280.10691263371953671No Hit
TGAGGTAGTAAGTTGTATTGTT71170.1067476450872535No Hit
TCGTTTCCCGGCCAATGCACCA70740.10610268952469175No Hit
GCCTGTCTGAGCGTCGCA69280.10391284040529608No Hit
CAACGGAATCCCAAAAGCAGCTGA67930.10188797991818364No Hit
CCCCCCACTGCTAAATTTGACTGGCT67650.10146800885418995No Hit
ATCCCACCGCTGCCACCA66950.10041808119420571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)