FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12892737
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG215887616.744900636691806No Hit
TAGCTTATCAGACTGATGTTGAC6305744.890924246728991No Hit
TCGTACGACTCTTAGCGG5027963.89983911096612No Hit
TAGCAGCACGTAAATATTGGCG4767363.697709803589416No Hit
AAGCTGCCAGTTGAAGAACTGT2929002.271821724122659No Hit
AACATTCAACGCTGTCGGTGAGT2907942.255486945867274No Hit
AACATTCAACGCTGTCGGTGAG2741572.1264453001717167No Hit
TTCAAGTAATCCAGGATAGGCT2519361.9540924475539987No Hit
TAGCTTATCAGACTGATGTTGA2407601.8674079832699604No Hit
CCCCCCACTGCTAAATTTGACTG2166781.680620647113177No Hit
CATAAAGTAGAAAGCACTACT1924781.4929180669705742No Hit
TAGCTTATCAGACTGATGTTGACT1557751.208238405855948No Hit
TGAGAACTGAATTCCATGGGTT1348121.0456429848836597No Hit
AGCAGCATTGTACAGGGCTATGA1259200.9766739211387No Hit
TGAGGTAGTAGGTTGTATAGTT1240490.9621618745499889No Hit
TATTGCACTTGTCCCGGCCTGT1193970.9260795438548075No Hit
CCCCCACTGCTAAATTTGACTG1140310.884459211414923No Hit
TAGCTTATCAGACTGATGTTGACA954570.7403936030029931No Hit
TGTAAACATCCTTGACTGGAAGCT884340.6859210732368154No Hit
TTAATGCTAATCGTGATAGGGGTT755000.5856010248250623No Hit
TATTGCACTTGTCCCGGCCTGTA751260.582700166768313No Hit
TTCACAGTGGCTAAGTTCCGC694190.5384349343355099No Hit
ACTGGACTTGGAGTCAGAAGGC630660.4891591288955945No Hit
TGAGGTAGTAGTTTGTGCTGTT601780.46675892015791526No Hit
CATTGCACTTGTCTCGGTCTGA581320.45088952020040435No Hit
TGAGAACTGAATTCCATAGGCTGT535830.4156060889165737No Hit
CAACGGAATCCCAAAAGCAGCT522040.4049101443704312No Hit
TGAGGTAGTAGATTGTATAGTT512760.39771229336330993No Hit
TAGCAGCACGTAAATATTGGC452390.35088748029219863No Hit
TTCAAGTAATTCAGGATAGGTT445960.34590017619997987No Hit
AACATTCAACGCTGTCGGTGAGTT441390.34235554483117125No Hit
AACGGAATCCCAAAAGCAGCTG431310.3345371894268843No Hit
TGTAAACATCCCCGACTGGAAGCT429090.33281528972474966No Hit
CAACACCAGTCGATGGGCTGT398530.30911202175302266No Hit
CCCATAAAGTAGAAAGCACTAC388580.30139449831327514No Hit
ACCGGGTGCTGTAGGCTT369730.28677386345506No Hit
TTCACCACCTTCTCCACCCAGC368170.285563879880587No Hit
CACCCGGCTGTGTGCACATGTGC357810.27752834793729214No Hit
CTCCCACTGCTTCACTTGACTAGCCT346140.26847674004363853No Hit
TATTGCACTTGTCCCGGCCTGTAT332680.2580367535613268No Hit
CCCCCACTGCTAAATTTGACTGGCTT332450.25785835854714173No Hit
AGCAGCATTGTACAGGGCTATG326470.25322008817832864No Hit
CCCATAAAGTAGAAAGCACT313920.2434859254477928No Hit
TGTAAACATCCTACACTCTCAGCT310790.24105820199388228No Hit
AATCCTTGGAACCTAGGTGTGAGT308800.23951469730593278No Hit
TAGCAGCACGTAAATATTGGCGT304310.2360321163768407No Hit
CAACGGAATCCCAAAAGCAGCTGT295860.22947803868177877No Hit
TCAGTGCATCACAGAACTTTGT293500.22764755071014012No Hit
CCCATAAAGTAGAAAGCACTA292120.2265771806250294No Hit
TGAGGTAGTAGGTTGTGTGGTT272260.21117315896539268No Hit
ACCGGGTGCTGTAGGCTTT259970.2016406601639357No Hit
AGCAGCATTGTACAGGGCTATGT255530.19819686075966647No Hit
CAAAGAATTCTCCTTTTGGGCT253890.1969248267454769No Hit
TATTGCACTTGTCCCGGCCTGTAA244220.1894244798447374No Hit
TATTGCACTTGTCCCGGCCTGTT231970.1799230062631387No Hit
TTCACAGTGGCTAAGTTCTGC227040.1760991479156055No Hit
CACTAGATTGTGAGCTCCTGGA212930.16515500161059674No Hit
CAAAGTGCTGTTCGTGCAGGTAG197970.15355156938359946No Hit
ACTGGACTTGGAGTCAGAAGG196760.15261305648288645No Hit
CACTAGATTGTGAGCTCCTGGAA188740.14639249990130102No Hit
CTCGTACGACTCTTAGCGG179770.1394350943480814No Hit
TGAGGTAGTAAGTTGTATTGTT178700.13860516971687237No Hit
CAAAGAATTCTCCTTTTGGGCTT174170.135091563567922No Hit
TGATAACTGAATTCCATGGGTT172870.13408324392252785No Hit
TGAGAACTGAATTCCATGGGTTG172660.13392036151827189No Hit
TCAGTGCATCACAGAACTTTGTT171980.1333929327806811No Hit
TGATGTAGTAGGTTGTATAGTT170800.13247768879486177No Hit
CGCGACCTCAGATCAGACGTGG167190.1296776627026519No Hit
TAGCAGCACATAATGGTTTGTG164510.12759897297214703No Hit
AACATTCATTGCTGTCGGTGGGTT158820.1231856354473065No Hit
ACCACAGGGTAGAACCACGGAC157820.12241000495084946No Hit
AGTGTTTCCTACTTTATGGATG157260.12197565187283352No Hit
TTCACAGTGGCTAAGTTCCG153860.1193385081848796No Hit
TGAGGTAGTAGTTTGTACAGTT149690.11610412901465375No Hit
ATCCCACCGCTGCCACCA145960.113211027262869No Hit
CAAAGAATTCTCCTTTTGGGC143950.11165200996499038No Hit
CACGCTCATGCACACACCCACA141200.1095190260997335No Hit
TTAATGCTAATCGTGATAGGGGT138960.10778161378766976No Hit
TGGCTCAGTTCAGCAGGAACAGT138180.10717662200043328No Hit
ATCACATTGCCAGGGATTTCC131540.10202643550395855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)