FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894604
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG77673011.265766677825152No Hit
TAGCAGCACGTAAATATTGGCG2911384.22269357311892No Hit
CCCCCCACTGCTAAATTTGACTG2794024.052473499565747No Hit
TTCAAGTAATCCAGGATAGGCT2660923.8594239785200135No Hit
GCCTGTCTGAGCGTCGCT2386333.4611559996774286No Hit
AAGCTGCCAGTTGAAGAACTGT2060692.9888446094946133No Hit
AACATTCAACGCTGTCGGTGAGT1470452.1327548326198285No Hit
AACATTCAACGCTGTCGGTGAG1279381.8556250656310356No Hit
CCCCCACTGCTAAATTTGACTG1133931.644662985720427No Hit
TATTGCACTTGTCCCGGCCTGTAA979821.4211403584600362No Hit
TGAGGTAGTAGGTTGTATAGTT948831.3761921641910109No Hit
TAGCTTATCAGACTGATGTTGAC873741.2672809054733236No Hit
TATTGCACTTGTCCCGGCCTGTAT799941.160240675171482No Hit
TCCTGTCTGAGCGTCGCT762831.106415973999377No Hit
TAGCTTATCAGACTGATGTTGACT739581.0726939502254227No Hit
CATAAAGTAGAAAGCACTACT723491.0493568593642217No Hit
TATTGCACTTGTCCCGGCCTGT623290.904025814970664No Hit
TGTAAACATCCTTGACTGGAAGCT619120.8979776068357226No Hit
TAGCTTATCAGACTGATGTTGA604650.8769901795665132No Hit
TGAGGTAGTAGATTGTATAGTT575040.8340435505795546No Hit
CAACGGAATCCCAAAAGCAGCTGT468700.6798069910904238No Hit
TTCAAGTAATTCAGGATAGGTT463730.6725984552557334No Hit
TATTGCACTTGTCCCGGCCTGTA422680.6130591401623646No Hit
AGCAGCATTGTACAGGGCTATGA382070.554158005303858No Hit
CCCATAAAGTAGAAAGCACTAC320530.4648997970006689No Hit
TCGTACGACTCTTAGCGG315350.4573866751447944No Hit
TGTAAACATCCCCGACTGGAAGCT312320.4529919339819952No Hit
TGATGTAGTAGGTTGTATAGTT293430.4255936961716728No Hit
CATTGCACTTGTCTCGGTCTGA282560.4098277435513338No Hit
CCCCCACTGCTAAATTTGACTGGCTT280680.40710097345692375No Hit
ACTGGACTTGGAGTCAGAAGGC249600.36202224232167646No Hit
TTCTCACTACTGCACTTGACTAG226840.3290109192638185No Hit
CACCCGGCTGTGTGCACATGTGC225810.3275169973503917No Hit
TGAGGTAGTAGTTTGTACAGTT216800.314448806631969No Hit
CCCATAAAGTAGAAAGCACT210530.3053547382851865No Hit
TAGCAGCACGTAAATATTGGC208780.3028165214419857No Hit
CTCCCACTGCTTCACTTGACTAG182110.2641340967516046No Hit
CAACGGAATCCCAAAAGCAGCT178810.2593477449901401No Hit
TGATGTAGTAGATTGTATAGTT176600.2561423397195836No Hit
TGTAAACATCCTACACTCTCAGCT176520.2560263069496087No Hit
CAAAGAATTCTCCTTTTGGGCT166980.24218939913010232No Hit
CCCCCCACTGCTAAATTTGACTGGCT157060.22780133565321517No Hit
AACCGGGCGGAAACACCA148860.2159079767307883No Hit
AACGGAATCCCAAAAGCAGCTG145810.2114842273754954No Hit
CTTCTCACTACTGCACTTGACTAG141360.2050299045456418No Hit
CAACACCAGTCGATGGGCTGT139680.20259321637616898No Hit
TTCACAGTGGCTAAGTTCCGC136810.19843054075331956No Hit
TTCAAGTAATCCAGGATAGGC136190.1975312867860141No Hit
TTCACAGTGGCTAAGTTCCG132580.1922953080408969No Hit
TGAGGTAGTAGTTTGTGCTGTT132360.19197621792346595No Hit
CGCCTGTCTGAGCGTCGCT130290.1889738700003655No Hit
TGTCAGTTTGTCAAATACCCCAA122100.1770950151741855No Hit
TAGCTTATCAGACTGATGTTGACA120210.17435374098352857No Hit
CAAAGAATTCTCCTTTTGGGCTT116710.16927730729712687No Hit
TATTGCACTTGTCCCGGCCTGTT115360.16731925430380049No Hit
TTCACAGTGGCTAAGTTCTGC114350.1658543405828674No Hit
AACATTCAACGCTGTCGGTGAGTT114310.16579632419787996No Hit
TGTAAACATCCCCGACTGGAAGC113410.1644909555356624No Hit
AGCTCGGTCTGAGGCCCCTCAGT112340.16293901723724813No Hit
TCGTTTCCCGGCCAATGCACCA108860.15789159174334016No Hit
ACCGGGTGCTGTAGGCTT108380.1571953951234908No Hit
TGTCAGTTTGTCAAATACCCCA108050.15671675994734433No Hit
TTCAAGTAATCCAGGATAGGCTT105510.15303271950064137No Hit
TATTGCACTTGTCCCGGCCTGTAG99920.14492492969864548No Hit
TAGCAGCACGTAAATATTGGCGT95200.1380789962701266No Hit
ACCCCCCACTGCTAAATTTGACTG94630.13725226278405547No Hit
ACCGGGTGCTGTAGGCTTA93690.13588887773685043No Hit
CACTAGATTGTGAGCTCCTGGA92640.13436594763092993No Hit
CACTAGATTGTGAGCTCCTGGAA90810.13171169801775418No Hit
ATCACATTGCCAGGGATTTCC89420.12969562863944037No Hit
TTATAAAGCAATGAGACTGATT89350.1295940999657123No Hit
CCCATAAAGTAGAAAGCACTA88500.12836125178472904No Hit
AGAATTACTCTGAGACCT86100.12488026868548216No Hit
ACTTGACTTGGAGTCAGAAGGC83660.12134126920124783No Hit
CCCCCCACTGCTAAATTTGACTGG83520.12113821185379176No Hit
ACCGGGTGCTGTAGGCTTT81580.11832441718190051No Hit
TATTGCACTTGTCCCGGCCTGTAAA81100.11762822056205113No Hit
CTCCCACTGCTTCACTTGACTAGCCT80750.11712057719341096No Hit
TGAGGTAGTAGGTTGTATAGT78320.1135960818054235No Hit
ATCCCACCGCTGCCACCA77850.11291438928182097No Hit
ATCCCGGACGAGCCCCCA76060.11031815605363267No Hit
TAGCAGCACATCATGGTTTACA75770.10989753726247367No Hit
CCCTGTCTGAGCGTCGCT75750.10986852906997993No Hit
CTACGCCTGTCTGAGCGTCGCT75370.10931737341259917No Hit
TTCACAGTGGCTAAGTTCTG73770.10699671801310126No Hit
TGTAAACATCCTACACTCAGCT72770.10554630838841504No Hit
CAAAGTGCTGTTCGTGCAGGTAG72740.10550279609967447No Hit
TGAGGTAGTAGGTTGTGTGGTT72690.10543027561844015No Hit
AGAGGTAGTAGGTTGCATAGTT71830.10418292334121002No Hit
CAAAGTGCTGTTCGTGCAGGT71350.10348672672136065No Hit
TTAATGCTAATCGTGATAGGGGTT69400.10065842795322255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)