FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7630529
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4414715.785588391053883No Hit
TAGCAGCACGTAAATATTGGCG3159804.140997301759812No Hit
AAGCTGCCAGTTGAAGAACTGT2894923.793865405661914No Hit
TTCAAGTAATCCAGGATAGGCT2736253.585924383486387No Hit
AACATTCAACGCTGTCGGTGAGT2722523.5679308734689297No Hit
CCCCCCACTGCTAAATTTGACTG2541033.330083667855793No Hit
TAGCTTATCAGACTGATGTTGAC1344321.7617651410537856No Hit
AACATTCAACGCTGTCGGTGAG1152841.5108257894046402No Hit
CCCCCACTGCTAAATTTGACTG963631.2628613297977112No Hit
TAGCTTATCAGACTGATGTTGACT904581.185474820946228No Hit
TATTGCACTTGTCCCGGCCTGT887671.1633138410194104No Hit
TATTGCACTTGTCCCGGCCTGTAA881811.155634163765055No Hit
TATTGCACTTGTCCCGGCCTGTAT867201.1364873916343154No Hit
TGAGGTAGTAGGTTGTATAGTT853041.117930355811504No Hit
TGTAAACATCCTTGACTGGAAGCT850381.1144443589690833No Hit
CATAAAGTAGAAAGCACTACT823051.0786277072009032No Hit
TAGCTTATCAGACTGATGTTGA780391.0227207052092981No Hit
AGCAGCATTGTACAGGGCTATGA579870.7599342063964373No Hit
TTCAAGTAATTCAGGATAGGTT578790.7585188392574093No Hit
TGAGGTAGTAGATTGTATAGTT515170.6751432305676317No Hit
TGTAAACATCCCCGACTGGAAGCT486520.6375966856295284No Hit
ACTGGACTTGGAGTCAGAAGGC466110.6108488677521572No Hit
CGCCTGTCTGAGCGTCGCT454980.5962627230693966No Hit
TATTGCACTTGTCCCGGCCTGTA432280.5665138026472345No Hit
CCCATAAAGTAGAAAGCACTAC370820.4859689282355129No Hit
CAACGGAATCCCAAAAGCAGCTGT340260.44591928030153605No Hit
CATTGCACTTGTCTCGGTCTGA314770.4125139947702184No Hit
TGTAAACATCCTACACTCTCAGCT300060.3932361701266059No Hit
TTCTCACTACTGCACTTGACTAG283660.37174355801544035No Hit
CCCCCACTGCTAAATTTGACTGGCTT249840.32742159816180505No Hit
AACATTCAACGCTGTCGGTGAGTT244630.3205937622411238No Hit
CTTCTCACTACTGCACTTGACTAG233850.30646630135341857No Hit
ACCGGGTGCTGTAGGCTT232440.30461846092190986No Hit
TGAGGTAGTAGTTTGTACAGTT227270.2978430460063778No Hit
CCCATAAAGTAGAAAGCACT197520.258854923426672No Hit
CCCCCCACTGCTAAATTTGACTGGCT194950.2554868738458369No Hit
TGATGTAGTAGGTTGTATAGTT193070.2530230866038252No Hit
TCGTACGACTCTTAGCGG192720.2525644028087699No Hit
CTACGCCTGTCTGAGCGTCGCT184090.24125457094783337No Hit
CACCCGGCTGTGTGCACATGTGC180680.23678568025886543No Hit
TGTCAGTTTGTCAAATACCCCAA167840.21995853760597728No Hit
TTCAAGTAATCCAGGATAGGCTT166750.21853006521566198No Hit
CGCGACCTCAGATCAGACGTGGCG163360.21408738502926863No Hit
TTATAAAGCAATGAGACTGATT163060.21369422749064976No Hit
CTCCCACTGCTTCACTTGACTAG161340.2114401242692348No Hit
CAAAGAATTCTCCTTTTGGGCT159760.20936949456584203No Hit
CAAAGAATTCTCCTTTTGGGCTT159530.2090680737862342No Hit
TGAGGTAGTAGTTTGTGCTGTT154900.2030003424402161No Hit
CACTAGATTGTGAGCTCCTGGA154680.2027120269118956No Hit
TACGCCTGTCTGAGCGTCGCT153900.20168981731148652No Hit
TAGCTTATCAGACTGATGTTGACA150060.19665740081716482No Hit
TGTAAACATCCCCGACTGGAAGC145130.19019651193252787No Hit
CAACGGAATCCCAAAAGCAGCT144150.18891219730637288No Hit
TGTAAACATCCTACACTCAGCT141050.18484956940731107No Hit
TGTCAGTTTGTCAAATACCCCA139970.1834342022682831No Hit
CGCGACCTCAGATCAGACGTGG135640.17775962846088392No Hit
TATTGCACTTGTCCCGGCCTGTT135090.1770388396400826No Hit
TAGCAGCACGTAAATATTGGC133710.17523031496243577No Hit
CCCCCCACTGCTAAATTTGACTGG130080.1704731087451473No Hit
GTCAGGATGGCCGAGCGGTCTAAGG129980.17034205623227433No Hit
ACGCCTGTCTGAGCGTCGCT128110.16789137424154998No Hit
CACTAGATTGTGAGCTCCTGGAA124540.16321279953198528No Hit
GGAATACCGGGTGCTGTAGGCTTT123210.16146980111077489No Hit
TAGCAGCACGTAAATATTGGCGT122100.16001511821788503No Hit
ACCGGGTGCTGTAGGCTTT121000.15857354057628245No Hit
TTCAAGTAATCCAGGATAGGC120780.15828522504796194No Hit
CAACACCAGTCGATGGGCTGT120190.15751201522201147No Hit
TTCACAGTGGCTAAGTTCCG120140.157446488965575No Hit
TTCACAGTGGCTAAGTTCCGC118610.1554413855186187No Hit
TGATGTAGTAGATTGTATAGTT116790.1530562297843308No Hit
ACTTGACTTGGAGTCAGAAGGC114740.1503696532704351No Hit
AGCTCGGTCTGAGGCCCCTCAGT113900.14926881216230226No Hit
AGCAGCATTGTACAGGGCTATGT110100.14428881667312973No Hit
CAAAGTGCTGTTCGTGCAGGTAG108780.14255892350320667No Hit
ACCACAGGGTAGAACCACGGAC106810.13997718899960931No Hit
CTCCCACTGCTTCACTTGACTAGCCT104310.1367008761777853No Hit
GGAATACCGGGTGCTGTAGGCTT102940.13490545675142576No Hit
ACTGGACTTGGAGTCAGAAGGCA102480.13430261519221015No Hit
TTCACAGTGGCTAAGTTCTGC101530.133057616319917No Hit
GCGTTCGCGCTTTCCCCTG97960.1283790416103523No Hit
ACCGGGTGCTGTAGGCTTA97790.12815625233846828No Hit
ACTGGACTTGGAGTCAGAAGG96880.12696367447132434No Hit
TATTGCACTTGTCCCGGCCTGTAG96620.12662293793785465No Hit
AGCAGCATTGTACAGGGCTATG93980.12316315159800847No Hit
ATCACATTGCCAGGGATTTCCA92480.12119736390491408No Hit
AACATTCAACGCTGTCGGTGA92240.12088283787401895No Hit
AACGGAATCCCAAAAGCAGCTG90710.11887773442706266No Hit
CAAAGTGCTGTTCGTGCAGGT90610.1187466819141897No Hit
ATCCCGGACGAGCCCCCA88910.11651878919534937No Hit
GCCTGTCTGAGCGTCGCT81700.10706990301720891No Hit
CGCGACCTCAGATCAGACGTGGC79910.1047240630367829No Hit
ACCCCCCACTGCTAAATTTGACTG79520.10421295823657836No Hit
CCCCCACTGCTAAATTTGACTGGCT78350.10267964383596472No Hit
TGAGGTAGTAGGTTGTATAGT77680.10180159199971586No Hit
TCGTTTCCCGGCCAATGCACCA77620.10172296049199209No Hit
TAGCAGCACATCATGGTTTACA77120.10106769792762729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)