FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7771764
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6011647.735232310193671No Hit
CCCCCCACTGCTAAATTTGACTG3829614.927594301628305No Hit
TTCAAGTAATCCAGGATAGGCT2419653.113385841361112No Hit
AACATTCAACGCTGTCGGTGAGT2077252.672816621811985No Hit
TAGCAGCACGTAAATATTGGCG1921452.4723473332437784No Hit
TATTGCACTTGTCCCGGCCTGTAT1778862.2888754728012843No Hit
AAGCTGCCAGTTGAAGAACTGT1606222.0667380018230097No Hit
AACATTCAACGCTGTCGGTGAG1553791.9992758400795494No Hit
TATTGCACTTGTCCCGGCCTGTAA1448711.8640684405753956No Hit
TGAGGTAGTAGGTTGTATAGTT1397811.7985749438608791No Hit
CCCCCACTGCTAAATTTGACTG1397221.7978157854510248No Hit
TAGCTTATCAGACTGATGTTGAC986151.2688882472499166No Hit
TGAGGTAGTAGATTGTATAGTT984481.2667394429372791No Hit
TATTGCACTTGTCCCGGCCTGT945781.2169437980875384No Hit
TAGCTTATCAGACTGATGTTGACT937281.2060067701489647No Hit
GCCTGTCTGAGCGTCGCT787671.0135022113383783No Hit
TGTAAACATCCTTGACTGGAAGCT721750.9286823429018175No Hit
TATTGCACTTGTCCCGGCCTGTA705810.908172198744069No Hit
AGCAGCATTGTACAGGGCTATGA670870.8632145803706855No Hit
TTCAAGTAATTCAGGATAGGTT664760.8553527873466049No Hit
ACTGGACTTGGAGTCAGAAGGC598360.769915298508807No Hit
TGTAAACATCCCCGACTGGAAGCT582670.7497268316433695No Hit
TAGCTTATCAGACTGATGTTGA573120.7374387590770898No Hit
GGAATACCGGGTGCTGTAGGCTTT449810.578774651417619No Hit
CATAAAGTAGAAAGCACTACT391940.5043127917934719No Hit
CAACGGAATCCCAAAAGCAGCTGT364810.4694043720318836No Hit
CCCCCACTGCTAAATTTGACTGGCTT361150.46469501647245076No Hit
GGAATACCGGGTGCTGTAGGCTT342360.44051775118235703No Hit
TGAGGTAGTAGTTTGTACAGTT332850.42828114698284714No Hit
CACCCGGCTGTGTGCACATGTGC304320.3915713343843174No Hit
ACCGGGTGCTGTAGGCTT294720.3792189263595755No Hit
CATTGCACTTGTCTCGGTCTGA284160.36563127753235947No Hit
ACCGGGTGCTGTAGGCTTA281470.36217002986709323No Hit
CCCATAAAGTAGAAAGCACTAC262960.3383530431443878No Hit
TTCTCACTACTGCACTTGACTAG258490.3326014531578674No Hit
TGAGAACTGAATTCCATGGGTT248920.32028764640820284No Hit
TTAATGCTAATCGTGATAGGGGTT248380.31959282345681106No Hit
CACTAGATTGTGAGCTCCTGGAA245990.31651758854231804No Hit
CACTAGATTGTGAGCTCCTGGA239650.30835985240931146No Hit
TGAGAACTGAATTCCATAGGCTGT233310.30020211627630483No Hit
CGCGACCTCAGATCAGACGTGGCG229250.2949780770491744No Hit
CTCCCACTGCTTCACTTGACTAG227580.292829272736537No Hit
CCCCCCACTGCTAAATTTGACTGGCT221220.28464580242014553No Hit
AGCTCGGTCTGAGGCCCCTCAGT216400.2784438642243897No Hit
TGAGGTAGTAGTTTGTGCTGTT208990.2689093492802921No Hit
TATTGCACTTGTCCCGGCCTGTAG208690.26852333652951893No Hit
ACCGGGTGCTGTAGGCTTT208180.26786711485320447No Hit
TATTGCACTTGTCCCGGCCTGTT198890.2559135866709283No Hit
ACTGGACTTGGAGTCAGAAGGCA195030.2509468892776466No Hit
CAAAGAATTCTCCTTTTGGGCT185890.23918636747075694No Hit
CTTCTCACTACTGCACTTGACTAG181010.23290722672484654No Hit
CAACGGAATCCCAAAAGCAGCT177670.22860961809957173No Hit
TATTGCACTTGTCCCGGCCTGTTT177490.22837801044910783No Hit
TGTAAACATCCTACACTCTCAGCT175450.22575312374385018No Hit
ATCCCGGACGAGCCCCCA174510.22454361712476084No Hit
CGCGACCTCAGATCAGACGTGG164220.21130337977324068No Hit
AACGGAATCCCAAAAGCAGCTG158640.20412354260885948No Hit
CCCATAAAGTAGAAAGCACT158610.20408494133378213No Hit
TGTAAACATCCCCGACTGGAAGC147260.18948079226286335No Hit
AACATTCAACGCTGTCGGTGAGTT146650.18869589966962455No Hit
ACCCCCCACTGCTAAATTTGACTG143200.18425675303573294No Hit
CCCCCCACTGCTAAATTTGACTGG142120.18286710713294949No Hit
TAGCTTATCAGACTGATGTTGACA139820.17990767604368843No Hit
CTCCCACTGCTTCACTTGACTAGCCT138450.1781448844818242No Hit
CAAAGAATTCTCCTTTTGGGCTT135630.17451636462455627No Hit
TATTGCACTTGTCCCGGCCTGTAAT135550.17441342789101677No Hit
TGAGGTAGTAGGTTGTATAGT134540.1731138516300804No Hit
TTCAAGTAATCCAGGATAGGCTT134230.17271497178761475No Hit
AACCGGGCGGAAACACCA132360.1703088256411286No Hit
TCGTACGACTCTTAGCGG132180.17007721799066466No Hit
TAGCAGCACGTAAATATTGGC129280.1663457613998572No Hit
TTATAAAGCAATGAGACTGATT122620.15777627833269256No Hit
AGCAGCATTGTACAGGGCTATGT120820.15546020182805345No Hit
ACTGGACTTGGAGTCAGAAGG118820.15288678348956555No Hit
ACTGGACTTGGAGTCAGAAGGCT117800.15157434013693674No Hit
CGCGACCTCAGATCAGACGTGGC113590.14615729453441972No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC113170.14561687668333728No Hit
CAACACCAGTCGATGGGCTGT112420.1446518448064043No Hit
TGTCAGTTTGTCAAATACCCCA112340.1445489080728648No Hit
GGGAATACCGGGTGCTGTAGGCTT109120.1404057045478993No Hit
TATTGCACTTGTCCCGGCCTGTAAA108250.13928626757065707No Hit
TGTCAGTTTGTCAAATACCCCAA105360.13556767807154205No Hit
TTCAAGTAATCCAGGATAGGC105310.13550334261307986No Hit
TGAGGTAGTAGGTTGTGTGGTT103430.13308432937490125No Hit
CAAAGTGCTGTTCGTGCAGGTAG101590.1307167845034924No Hit
GGAATACCAGGTGCTGTAGGCTTT100820.12972601844317455No Hit
TTCACAGTGGCTAAGTTCCGC94970.12219876980309748No Hit
AGCTACATCTGGCTACTGGGTCTC94690.12183849123570917No Hit
TCGTTTCCCGGGCGGCGCACCA93610.1204488453329257No Hit
TGAGGTAGTAGATTGTATAGT88690.1141182362202455No Hit
TTCACAGTGGCTAAGTTCTGC86090.11077279238021125No Hit
CGCCTGTCTGAGCGTCGCT85660.11021950743743635No Hit
GGGAATACCGGGTGCTGTAGGCTTT85420.1099106972368178No Hit
GGAATACCGGGTGCTGTAGGCTTA85150.10956328576112193No Hit
TGAGGTAGTAAGTTGTATTGTT84640.1089070640848075No Hit
CCCCCACTGCTAAATTTGACTGGCT84180.1083151778669553No Hit
TATTGCACTTGTCCCGGCCTGA83070.10688693068909452No Hit
GTCAGGATGGCCGAGCGGTCTAAGG82660.10635937992970451No Hit
TGAGGTAGTAGGTTGTATAGTTA81420.10476386055984202No Hit
TTCACCACCTTCTCCACCCAGC81190.10446791745091591No Hit
CCCCCCACTGCTAAATTTGACTGT80390.10343855011552075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)