FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345071
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6866549.348500511431409No Hit
CCCCCCACTGCTAAATTTGACTG4018425.4709069524310925No Hit
AACATTCAACGCTGTCGGTGAGT2543233.4624988648850366No Hit
TTCAAGTAATCCAGGATAGGCT2209393.007989984031468No Hit
TAGCAGCACGTAAATATTGGCG2173142.958637159531882No Hit
AAGCTGCCAGTTGAAGAACTGT1860522.533018401047451No Hit
AACATTCAACGCTGTCGGTGAG1850472.5193357559103244No Hit
CCCCCACTGCTAAATTTGACTG1650242.2467311752330237No Hit
TATTGCACTTGTCCCGGCCTGTAA905011.2321324055274618No Hit
TATTGCACTTGTCCCGGCCTGTAT840881.1448221535230905No Hit
TAGCTTATCAGACTGATGTTGAC808141.100248043892292No Hit
TGAGGTAGTAGGTTGTATAGTT771071.0497788244660944No Hit
TAGCTTATCAGACTGATGTTGACT705590.960630605204497No Hit
TATTGCACTTGTCCCGGCCTGT696500.9482549590058422No Hit
TAGCTTATCAGACTGATGTTGA562330.7655882427821323No Hit
TGTAAACATCCTTGACTGGAAGCT537310.7315245829482111No Hit
TTCAAGTAATTCAGGATAGGTT498020.6780329284767975No Hit
TATTGCACTTGTCCCGGCCTGTA454420.6186733933545366No Hit
CATAAAGTAGAAAGCACTACT451620.6148613131173273No Hit
TGAGGTAGTAGATTGTATAGTT431790.5878636162945192No Hit
TGTAAACATCCCCGACTGGAAGCT397340.5409614148045676No Hit
AGCAGCATTGTACAGGGCTATGA376980.5132421456511448No Hit
TTAATGCTAATCGTGATAGGGGTT365040.4969863463539019No Hit
CAACGGAATCCCAAAAGCAGCTGT357750.4870613231648816No Hit
ACCGGGTGCTGTAGGCTT345620.47054684699439936No Hit
ACTGGACTTGGAGTCAGAAGGC287560.39150064036140697No Hit
TTCTCACTACTGCACTTGACTAG275010.37441435215534336No Hit
CCCCCACTGCTAAATTTGACTGGCTT269290.36662681681361553No Hit
CATTGCACTTGTCTCGGTCTGA263250.3584036151590638No Hit
CACCCGGCTGTGTGCACATGTGC258030.35129680843112343No Hit
GGAATACCGGGTGCTGTAGGCTTT241110.32826095214055795No Hit
TGAGAACTGAATTCCATAGGCTGT232370.3163618159715542No Hit
CAACGGAATCCCAAAAGCAGCT229640.3126450377402751No Hit
TGAGAACTGAATTCCATGGGTT222730.3032373682977333No Hit
CCCATAAAGTAGAAAGCACTAC222000.30224350452160365No Hit
TGATGTAGTAGGTTGTATAGTT211710.28823410964985907No Hit
GGAATACCGGGTGCTGTAGGCTT208630.28404082138892867No Hit
ACCGGGTGCTGTAGGCTTT207740.28282912445638714No Hit
ACCGGGTGCTGTAGGCTTA202480.27566785943934374No Hit
AACATTCAACGCTGTCGGTGAGTT185330.25231886798643605No Hit
TGAGGTAGTAGTTTGTACAGTT180140.2452529049753229No Hit
CCCATAAAGTAGAAAGCACT171880.23400726827555512No Hit
CTTCTCACTACTGCACTTGACTAG170410.23200592615102017No Hit
GCCTGTCTGAGCGTCGCT166200.22627419122293033No Hit
CAAAGAATTCTCCTTTTGGGCT160730.2188270201880962No Hit
CAACACCAGTCGATGGGCTGT159860.21764255240010613No Hit
TGTCAGTTTGTCAAATACCCCAA152290.20733632118736497No Hit
ACCCCCCACTGCTAAATTTGACTG151560.20634245741123539No Hit
TAGCAGCACGTAAATATTGGC144660.19694840254096932No Hit
CTCCCACTGCTTCACTTGACTAG135250.18413709002949052No Hit
TCGTACGACTCTTAGCGG133070.18116911327337748No Hit
AACGGAATCCCAAAAGCAGCTG132160.17993018719628442No Hit
CACTAGATTGTGAGCTCCTGGA127800.17399423368405834No Hit
TGTAAACATCCTACACTCTCAGCT123900.16868455049651665No Hit
TGAGGTAGTAGTTTGTGCTGTT121470.1653762094335099No Hit
CCCCCCACTGCTAAATTTGACTGG121400.16528090742757967No Hit
TATTGCACTTGTCCCGGCCTGTT120560.16413728335641684No Hit
TGATGTAGTAGATTGTATAGTT118780.16171388949133372No Hit
CCCCCCACTGCTAAATTTGACTGGCT115520.15727553892943988No Hit
TGTCAGTTTGTCAAATACCCCA114540.1559413108464166No Hit
CGCCTGTCTGAGCGTCGCT112930.15374936471002118No Hit
CTACGCCTGTCTGAGCGTCGCT112620.153327312969473No Hit
CGCGACCTCAGATCAGACGTGGCG112360.15297333409030356No Hit
TGTAAACATCCCCGACTGGAAGC109430.14898426441350943No Hit
CAAAGAATTCTCCTTTTGGGCTT109340.14886173326302768No Hit
CGCGACCTCAGATCAGACGTGG108810.14814016093241306No Hit
TTCACAGTGGCTAAGTTCCG108670.1479495569205526No Hit
CACTAGATTGTGAGCTCCTGGAA108270.14740497402952266No Hit
TCGTTTCCCGGCCAATGCACCA108220.1473369011681439No Hit
CTCCCACTGCTTCACTTGACTAGCCT106970.14563507963367542No Hit
TATTGCACTTGTCCCGGCCTGTAG104490.14225866570928994No Hit
TGAATACCGGGTGCTGTAGGCTTT98210.13370871432012024No Hit
TAGCTTATCAGACTGATGTTGACA96980.13203412193020325No Hit
AACCGGGCGGAAACACCA95430.12992386322746233No Hit
ATCCCGGACGAGCCCCCA94050.12804505225340912No Hit
GGGAATACCGGGTGCTGTAGGCTT93830.12774553166334268No Hit
TTCAAGTAATCCAGGATAGGCTT92610.1260845538457014No Hit
TTCACAGTGGCTAAGTTCTGC91330.12434188859440569No Hit
TTCACAGTGGCTAAGTTCTG90620.1233752539628276No Hit
TTCAAGTAATCCAGGATAGGC88920.12106077667595044No Hit
ACTTGACTTGGAGTCAGAAGGC88030.11984907974340887No Hit
ACCTGGTGCTGTAGGCTT87770.11949510086423944No Hit
AACATTCAACGCTGTCGGTGA82520.11234745041947178No Hit
TTATAAAGCAATGAGACTGATT81720.11125828463741194No Hit
TGAATACCGGGTGCTGTAGGCTT81120.11044141030086707No Hit
TTCACAGTGGCTAAGTTCCGC79740.10856259932681385No Hit
AGCTACATTGTCTGCTGGGTTT78490.10686077779234537No Hit
AGCTCGGTCTGAGGCCCCTCAGT78090.10631619490131544No Hit
ACTGGACTTGGAGTCAGAAGGCA77140.1050228105351194No Hit
CAAAGTGCTGTTCGTGCAGGTAG73830.10051638711184685No Hit
ACTGGACTTGGAGTCAGAAGGCT73460.10001264793764415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)