FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6716867
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT181761327.06042861947393No Hit
CAACGGAATCCCAAAAGCAGCTG3110394.630715480893101No Hit
CCCCCCACTGCTAAATTTGACTG2475743.6858553251091615No Hit
TTCAAGTAATCCAGGATAGGCT2217843.3018965538546468No Hit
TAGCAGCACGTAAATATTGGCG1908302.84105670098872No Hit
CATAAAGTAGAAAGCACTACT1641702.4441454624604No Hit
AAGCTGCCAGTTGAAGAACTGT1420762.1152123452794287No Hit
CCCCCACTGCTAAATTTGACTG1232141.8343968996259716No Hit
TGAGGTAGTAGGTTGTATAGTT1148151.7093534828067907No Hit
AACATTCAACGCTGTCGGTGAGT1012781.5078160696050704No Hit
TGAGGTAGTAGATTGTATAGTT826011.229754884233974No Hit
TAGCTTATCAGACTGATGTTGA817771.2174872600574047No Hit
TAGCTTATCAGACTGATGTTGAC783581.1665855524606934No Hit
AACATTCAACGCTGTCGGTGAG776191.1555833992246682No Hit
TCCTGTCTGAGCGTCGCT753481.1217729932720122No Hit
TTCAAGTAATTCAGGATAGGTT672441.0011215050111906No Hit
TCGTACGACTCTTAGCGG648060.9648248208577005No Hit
TAGCTTATCAGACTGATGTTGACT529490.7882990685985No Hit
TGTAAACATCCTTGACTGGAAGCT457760.6815082091099913No Hit
TATTGCACTTGTCCCGGCCTGT353680.5265550144137141No Hit
TGAGGTAGTAGTTTGTACAGTT320280.4768294503970378No Hit
TATTGCACTTGTCCCGGCCTGTAA289590.4311385054966847No Hit
TATTGCACTTGTCCCGGCCTGTAT285240.4246622718597822No Hit
TTCACAGTGGCTAAGTTCCGC243330.3622671105442463No Hit
AACCGGGCGGAAACACCA231180.344178320041174No Hit
CAACACCAGTCGATGGGCTGT225620.33590065130067337No Hit
TTCTCACTACTGCACTTGACTAG221690.3300497091873339No Hit
CATTGCACTTGTCTCGGTCTGA208580.3105316809161176No Hit
AGCAGCATTGTACAGGGCTATGA184230.2742796604428821No Hit
CAACGGAATCCCAAAAGCAGCTGT181260.2698579560976866No Hit
CGCCTGTCTGAGCGTCGCT175570.26138674474274987No Hit
TTATAAAGCAATGAGACTGATT172970.25751589245402656No Hit
TATTGCACTTGTCCCGGCCTGTA166240.24749634018360048No Hit
CCCATAAAGTAGAAAGCACTAC164630.2450993893432757No Hit
TGAGGTAGTAGTTTGTGCTGTT162170.2414369675624067No Hit
TAGTGGGTTATCAGAACT156720.23332306564950592No Hit
TAGCAGCACGTAAATATTGGC152530.22708503830729415No Hit
TGTAAACATCCCCGACTGGAAGCT150680.22433077802493334No Hit
GCCTGTCTGAGCGTCGCA150420.22394369279606102No Hit
ACTGGACTTGGAGTCAGAAGGC149290.2222613608398082No Hit
TGTCAGTTTGTCAAATACCCCAA149250.22220180926613553No Hit
TGTCAGTTTGTCAAATACCCCA146550.21818207804323056No Hit
GCCTGTCTGAGCGTCGCG145470.21657418555406857No Hit
GCCGGTCTGAGCGTCGCT128110.1907288025801315No Hit
ACCGGGTGCTGTAGGCTT124980.18606889194024537No Hit
TGATGTAGTAGGTTGTATAGTT123700.18416324158272004No Hit
CTTCTCACTACTGCACTTGACTAG122060.18172162706214073No Hit
TTCACAGTGGCTAAGTTCCG121480.18085812924388708No Hit
TGAGGTAGTAAGTTGTATTGTT116530.17348862200189463No Hit
TTCACAGTGGCTAAGTTCTGC111770.16640198473484735No Hit
CCCTGTCTGAGCGTCGCT109770.16342440605121406No Hit
TGTAAACATCCTACACTCTCAGCT109440.16293310556841456No Hit
TGAGGTAGTAGGTTGTATAGT107180.15956844165590894No Hit
TTCAAGTAATCCAGGATAGGC104340.15534027992514962No Hit
CACCCGGCTGTGTGCACATGTGC101330.1508590240062815No Hit
CAACGGAATCCCAAAAGCAGCT95160.14167319376727275No Hit
TGATGTAGTAGATTGTATAGTT89550.13332108555968133No Hit
CTCCCACTGCTTCACTTGACTAG87360.13006063690110284No Hit
TGAGGTAGTAGATTGTATAGT86690.12906314804208568No Hit
TTCACAGTGGCTAAGTTCCGCT85840.12779767710154155No Hit
CAAAGAATTCTCCTTTTGGGCT85720.12761902238052353No Hit
AACGGAATCCCAAAAGCAGCTG85500.12729148872532386No Hit
TCAGTGCACTACAGAACTTTGT83650.12453722844296307No Hit
TGTCAGTTTGTCAAATACC80980.12056216090031259No Hit
AGAATTACTCTGAGACCT80710.12016018777802209No Hit
TTCACAGTGGCTAAGTTCTG80670.12010063620434945No Hit
TTCAAGTAATCCAGGATAGGCTT80060.11919247470584128No Hit
TCGACCGGACCTCGACCGGCT79660.11859695896911462No Hit
TAGCAGCACATAATGGTTTGT77850.11590225026042648No Hit
CCCCCACTGCTAAATTTGACTGGCTT77740.11573848343282664No Hit
TAGCAGCACGTAAATATTGGCGT75850.11292467157679317No Hit
GCCTGTCTGAGCGTCGCTT75000.11165920063624903No Hit
TGTCAGTTTGTCAAATACCCC73900.11002153236025068No Hit
TGTAAACATCCTACACTCAGCT71580.10656754108723605No Hit
TAGCTTATCAGACTGATGTTGACA70590.10509363963883755No Hit
CAAAGAATTCTCCTTTTGGGCTT70310.10467677862312892No Hit
CTACGCCTGTCTGAGCGTCGCT70270.10461722704945625No Hit
ACCGGGTGCTGTAGGCTTT69850.10399193552589325No Hit
CCCCCCACTGCTAAATTTGACTGG68120.10141632996455044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)