FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9245053
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7617108.239109067303346No Hit
CCCCCCACTGCTAAATTTGACTG7299777.8958660377609515No Hit
TTCAAGTAATCCAGGATAGGCT4515004.883692932858254No Hit
TAGCAGCACGTAAATATTGGCG3980644.305697328073728No Hit
CCCCCACTGCTAAATTTGACTG2817343.0474027569122644No Hit
AAGCTGCCAGTTGAAGAACTGT2577842.7883452912600935No Hit
GCCTGTCTGAGCGTCGCT2200532.3802243210504037No Hit
TAGCTTATCAGACTGATGTTGAC1914682.0710319345924786No Hit
CATAAAGTAGAAAGCACTACT1803141.950383626789376No Hit
TGAGGTAGTAGGTTGTATAGTT1584071.713424466036052No Hit
AACATTCAACGCTGTCGGTGAGT1439531.5570813926107292No Hit
TAGCTTATCAGACTGATGTTGACT1423141.5393529923516935No Hit
TAGCTTATCAGACTGATGTTGA1370611.4825334154385055No Hit
TGAGGTAGTAGATTGTATAGTT1300771.4069903114671165No Hit
AACATTCAACGCTGTCGGTGAG1279321.3837887138126737No Hit
TGTAAACATCCTTGACTGGAAGCT1140971.2341411130904278No Hit
TTCAAGTAATTCAGGATAGGTT1108851.1993982078848007No Hit
TATTGCACTTGTCCCGGCCTGTAA869150.9401244103197678No Hit
TATTGCACTTGTCCCGGCCTGTAT833540.9016065132346996No Hit
TATTGCACTTGTCCCGGCCTGT574170.6210564720396952No Hit
TTCTCACTACTGCACTTGACTAG493820.5341451260474115No Hit
AGCAGCATTGTACAGGGCTATGA478260.5173145032267528No Hit
AACCGGGCGGAAACACCA475070.5138640092166048No Hit
TGAGGTAGTAGTTTGTACAGTT456720.494015556211522No Hit
CAACGGAATCCCAAAAGCAGCTGT455730.49294471324285544No Hit
CCCATAAAGTAGAAAGCACTAC392800.4248758768608465No Hit
TATTGCACTTGTCCCGGCCTGTA385100.41654709821566194No Hit
TGTAAACATCCCCGACTGGAAGCT354790.3837619968214352No Hit
CATTGCACTTGTCTCGGTCTGA347750.376147113488695No Hit
ACTGGACTTGGAGTCAGAAGGC323420.3498303362890402No Hit
TGTCAGTTTGTCAAATACCCCA314670.3403658151013304No Hit
CTTCTCACTACTGCACTTGACTAG313020.33858107682021943No Hit
TCCTGTCTGAGCGTCGCT304990.3298953505188126No Hit
TAGCAGCACGTAAATATTGGC300160.3246709348231968No Hit
CCCCCACTGCTAAATTTGACTGGCTT279780.3026267129025653No Hit
TGTAAACATCCTACACTCTCAGCT276080.29862457251461944No Hit
CTCCCACTGCTTCACTTGACTAG274900.297348214228734No Hit
TGTCAGTTTGTCAAATACCCCAA266770.28855432197089625No Hit
TTATAAAGCAATGAGACTGATT246970.2671374625975643No Hit
TTCACAGTGGCTAAGTTCCGC246860.2670184800454903No Hit
CAAAGAATTCTCCTTTTGGGCT246110.266207235372258No Hit
TAGCTTATCAGACTGATGTTGACA219840.23779203861784243No Hit
TGAGGTAGTAGTTTGTGCTGTT214030.2315075965492031No Hit
TGAGAACTGAATTCCATGGGTT202470.21900361198578308No Hit
TTCAAGTAATCCAGGATAGGC196560.21261100396071284No Hit
TTCACAGTGGCTAAGTTCTGC194230.21009073717587123No Hit
TGAGAACTGAATTCCATAGGCTGT190110.20563429977091532No Hit
CACCCGGCTGTGTGCACATGTGC189200.2046499895673935No Hit
TTCAAGTAATCCAGGATAGGCTT184920.200020486632148No Hit
CAACGGAATCCCAAAAGCAGCT183690.1986900453680471No Hit
TGATGTAGTAGGTTGTATAGTT181330.19613732879627624No Hit
CCCCCCACTGCTAAATTTGACTGG180560.19530445093175777No Hit
CCCCCCACTGCTAAATTTGACTGGCT173080.18721363739072128No Hit
CAAAGAATTCTCCTTTTGGGCTT171600.18561278123554295No Hit
AACGGAATCCCAAAAGCAGCTG169960.18383885955007506No Hit
TCGTACGACTCTTAGCGG165100.17858199406752995No Hit
ACCCCCCACTGCTAAATTTGACTG163210.17653765749098466No Hit
TGTCAGTTTGTCAAATACCCC162840.17613744345219004No Hit
GGAATACCGGGTGCTGTAGGCTTT159080.1720704034903856No Hit
CAACACCAGTCGATGGGCTGT157660.17053444690906586No Hit
TCAGTGCACTACAGAACTTTGT153550.16608882609975303No Hit
ATCACATTGCCAGGGATTTCC148450.1605723623217736No Hit
TGATGTAGTAGATTGTATAGTT147950.16003153253961877No Hit
TAGCAGCACGTAAATATTGGCGT147220.15924192105767268No Hit
TTCACAGTGGCTAAGTTCCG139100.150458845395478No Hit
TGAGGTAGTAAGTTGTATTGTT138670.14999373178282482No Hit
TAGTGGGTTATCAGAACT128990.139523267200307No Hit
TGAGGTAGTAGGTTGTATAGT128480.13897162082250908No Hit
TGTAAACATCCTACACTCAGCT126930.13729504849782906No Hit
AGAATTACTCTGAGACCT119670.1294422000609407No Hit
TGTAAACATCCCCGACTGGAAGC118960.12867422177028082No Hit
GGAATACCGGGTGCTGTAGGCTT117310.12688948348916984No Hit
AGCTCGGTCTGAGGCCCCTCAGT114190.1235147056485236No Hit
CTCCCACTGCTTCACTTGACTAGCCT113960.12326592394873237No Hit
TCAGTGCACTACAGAACTTTGTC113850.1231469413966583No Hit
CACTAGATTGTGAGCTCCTGGA113600.12287652650558088No Hit
TTAATGCTAATCGTGATAGGGGTT110730.11977216355601207No Hit
TAGCAGCACATCATGGTTTACA110730.11977216355601207No Hit
TATTGCACTTGTCCCGGCCTGTT107690.11648391848051061No Hit
CTACGCCTGTCTGAGCGTCGCT105520.11413671722595858No Hit
TGAGGTAGTAGATTGTATAGT104790.1133471057440125No Hit
TTCACAGTGGCTAAGTTCTG102340.11069703981145375No Hit
TAGCAGCACATAATGGTTTGT101390.10966946322535955No Hit
TGTCAGTTTGTCAAATACCC100090.10826330579175696No Hit
TGTAAACATCCTTGACTGGAAGC99050.10713837984487487No Hit
TTCAAGTAATTCAGGATAGGTTA98980.1070626636753732No Hit
AGCAGCATTGTACAGGGCTATGT98740.10680306537993887No Hit
CACTAGATTGTGAGCTCCTGGAA97410.10536445815940698No Hit
AGAGGTAGTAGGTTGCATAGTT96550.10443423093410066No Hit
TATTGCACTTGTCCCGGCCTGTAG95810.10363380285651148No Hit
AACATTCAACGCTGTCGGTGAGTT94770.1025088769096294No Hit
ATCACATTGCCAGGGATTTCCA94350.10205457989261933No Hit
TGTAGTGTTTCCTACTTTATGGA94260.10195723053183145No Hit
AGTGTTTCCTACTTTATGGATG93120.10072413862851841No Hit
CGCCTGTCTGAGCGTCGCT92770.10034555778101002No Hit
CCCATAAAGTAGAAAGCACT92480.10003187650736022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)