FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7355595
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4437866.033312057012384No Hit
CAACGGAATCCCAAAAGCAGCTG4284695.825076013565185No Hit
GCCTGTCTGAGCGTCGCT3031224.120971858836708No Hit
TAGCAGCACGTAAATATTGGCG3001524.080594431857653No Hit
AAGCTGCCAGTTGAAGAACTGT2724213.703588900693962No Hit
TTCAAGTAATCCAGGATAGGCT2577933.5047198765021728No Hit
AACATTCAACGCTGTCGGTGAGT1904182.5887504681810243No Hit
CCCCCACTGCTAAATTTGACTG1541112.095153417228654No Hit
TAGCTTATCAGACTGATGTTGAC1338081.8191322387923752No Hit
AACATTCAACGCTGTCGGTGAG969931.3186288804644628No Hit
TGTAAACATCCTTGACTGGAAGCT872961.186797261132512No Hit
CATAAAGTAGAAAGCACTACT795301.0812177668835763No Hit
TAGCTTATCAGACTGATGTTGACT782971.0644550168953022No Hit
TGAGGTAGTAGGTTGTATAGTT734450.9984916244029205No Hit
TATTGCACTTGTCCCGGCCTGTAA714360.971179082045708No Hit
TATTGCACTTGTCCCGGCCTGTAT686680.9335478639049594No Hit
AACCGGGCGGAAACACCA669950.9108032728827511No Hit
TAGCTTATCAGACTGATGTTGA661780.8996960816901964No Hit
TATTGCACTTGTCCCGGCCTGT643100.8743004474824946No Hit
AGCAGCATTGTACAGGGCTATGA549020.7463978101023778No Hit
TTCAAGTAATTCAGGATAGGTT524430.712967475778642No Hit
TGAGGTAGTAGATTGTATAGTT501510.6818075220291492No Hit
CGCCTGTCTGAGCGTCGCT495720.6739359630322225No Hit
ACTGGACTTGGAGTCAGAAGGC381930.5192373968387329No Hit
TGTAAACATCCCCGACTGGAAGCT365250.4965607812828194No Hit
TGTAAACATCCTACACTCTCAGCT361250.491122743979243No Hit
CCCATAAAGTAGAAAGCACTAC355130.4828025469047711No Hit
GGAATACCGGGTGCTGTAGGCTTT346330.470838864836903No Hit
TATTGCACTTGTCCCGGCCTGTA339810.46197486403207355No Hit
TTCTCACTACTGCACTTGACTAG328960.44722418784612256No Hit
CAACGGAATCCCAAAAGCAGCTGT302190.4108301231919376No Hit
TCGTACGACTCTTAGCGG268190.36460680611153823No Hit
TCCTGTCTGAGCGTCGCT249220.3388169141993272No Hit
CTTCTCACTACTGCACTTGACTAG244900.33294383391146465No Hit
CATTGCACTTGTCTCGGTCTGA244240.3320465577563746No Hit
GGAATACCGGGTGCTGTAGGCTT234760.3191584093468985No Hit
CCCATAAAGTAGAAAGCACT227400.30915242070831794No Hit
TGAGGTAGTAGTTTGTACAGTT218750.297392665039334No Hit
CTACGCCTGTCTGAGCGTCGCT216540.29438814942910807No Hit
TACGCCTGTCTGAGCGTCGCT197200.26809523906631616No Hit
TGTCAGTTTGTCAAATACCCCA196280.2668444904864936No Hit
TGTCAGTTTGTCAAATACCCCAA189340.25740949576478855No Hit
AGCTCGGTCTGAGGCCCCTCAGT179980.24468448847441981No Hit
TGTAAACATCCTACACTCAGCT172060.23391717461333858No Hit
CTCCCACTGCTTCACTTGACTAG169370.23026009452668342No Hit
ACCGGGTGCTGTAGGCTT160420.21809248605993126No Hit
GCGTTCGCGCTTTCCCCTG157930.21470730783845496No Hit
AACATTCAACGCTGTCGGTGAGTT155280.21110460812483559No Hit
TTCAAGTAATCCAGGATAGGCTT151820.206400705857242No Hit
TAGCAGCACGTAAATATTGGC151500.2059656628729559No Hit
ACGCCTGTCTGAGCGTCGCT148650.20209106129415771No Hit
TAGCTTATCAGACTGATGTTGACA139530.18969233624200355No Hit
CACCCGGCTGTGTGCACATGTGC136930.1861576119946789No Hit
CCCCCCACTGCTAAATTTGACTGG135820.18464855664293642No Hit
TGAGGTAGTAGTTTGTGCTGTT133190.18107304711583494No Hit
TTAATGCTAATCGTGATAGGGGTT132870.18063800413154885No Hit
TGTCAGTTTGTCAAATACCCC131270.1784627892101183No Hit
CAAAGAATTCTCCTTTTGGGCT127640.1735277703571227No Hit
TGTAAACATCCCCGACTGGAAGC125810.1710398682907365No Hit
CAACGGAATCCCAAAAGCAGCT124010.16859275150412714No Hit
CAAAGAATTCTCCTTTTGGGCTT120650.16402480016912296No Hit
TTATAAAGCAATGAGACTGATT119100.1619175607139871No Hit
TAGCAGCACGTAAATATTGGCGT119050.1618495852476924No Hit
TTCAAGTAATCCAGGATAGGC117320.15949763411389561No Hit
ACCGGGTGCTGTAGGCTTT116850.15885866473072538No Hit
ATCCCGGACGAGCCCCCA112560.15302636972263972No Hit
CCCCCCACTGCTAAATTTGACTGGCT112240.1525913267383536No Hit
GGGCTACGCCTGTCTGAGCGTCGCT106990.14545390277740958No Hit
AACGGAATCCCAAAAGCAGCTG106750.145127620539195No Hit
CAACACCAGTCGATGGGCTGT106100.14424393947736383No Hit
TTCACAGTGGCTAAGTTCCG104780.14244938716718364No Hit
TATTGCACTTGTCCCGGCCTGTT104150.14159289629187033No Hit
CCCCCACTGCTAAATTTGACTGGCTT103270.14039652808508352No Hit
AGCAGCATTGTACAGGGCTATGT103010.14004305566035108No Hit
ACCACAGGGTAGAACCACGGAC98960.13453704289047996No Hit
ACCGGGTGCTGTAGGCTTA94910.12903103012060888No Hit
TTCACAGTGGCTAAGTTCTGC94860.12896305465431415No Hit
GAATACCGGGTGCTGTAGGCTTT92500.1257546126452041No Hit
ACCCCCCACTGCTAAATTTGACTG90050.12242381479676354No Hit
CACTAGATTGTGAGCTCCTGGA86790.11799181439434879No Hit
TTCACAGTGGCTAAGTTCCGC85640.11642837866957059No Hit
AGCAGCATTGTACAGGGCTATG85150.11576221909988246No Hit
AGAGGTAGTAGGTTGCATAGTT84460.11482415766501555No Hit
TATTGCACTTGTCCCGGCCTGTAG84390.11472899201220295No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT82950.11277129858291546No Hit
TGAGGTAGTAGGTTGTGTGGTT81070.11021542105023455No Hit
TGTAAACATCCTTGACTGGAAGC80440.10935893017492126No Hit
CAAAGTGCTGTTCGTGCAGGTAG79900.10862479513893847No Hit
ACTGGACTTGGAGTCAGAAGGCA79300.107809089543402No Hit
TAGCAGCACATAATGGTTTGT78500.10672148208268671No Hit
CAAAGTGCTGTTCGTGCAGGT77960.1059873470467039No Hit
TCAGTGCACTACAGAACTTTGT77580.10547073350286415No Hit
AATGACACGATCACTCCCGTTG76680.10424717510955947No Hit
ATCACATTGCCAGGGATTTCCA76260.10367618119268393No Hit
ACTGGACTTGGAGTCAGAAGG75350.1024390277061203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)