FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6992702
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89733312.832421573234495No Hit
CAACGGAATCCCAAAAGCAGCTG5981158.553417548752972No Hit
CCCCCACTGCTAAATTTGACTG3009764.304144520959137No Hit
TTCAAGTAATCCAGGATAGGCT2296783.2845386518687625No Hit
TAGCAGCACGTAAATATTGGCG2285633.2685934564350085No Hit
TAGCTTATCAGACTGATGTTGAC1651452.361676502158965No Hit
AACATTCAACGCTGTCGGTGAGT1631952.3337902859295308No Hit
GCCTGTCTGAGCGTCGCT1541382.2042695370115872No Hit
AAGCTGCCAGTTGAAGAACTGT1535732.196189684616905No Hit
TAGCTTATCAGACTGATGTTGACT1269181.8150065596960947No Hit
AACATTCAACGCTGTCGGTGAG1150021.644600327598688No Hit
TGTAAACATCCTTGACTGGAAGCT1019571.4580486913356239No Hit
TATTGCACTTGTCCCGGCCTGTAA804241.1501133610441285No Hit
TGAGGTAGTAGGTTGTATAGTT793361.1345542824504748No Hit
TAGCTTATCAGACTGATGTTGA693390.9915909472475732No Hit
TATTGCACTTGTCCCGGCCTGTAT662980.9481027505533627No Hit
CATAAAGTAGAAAGCACTACT548720.7847038240725831No Hit
TGAGGTAGTAGATTGTATAGTT509310.7283450660417103No Hit
AGCAGCATTGTACAGGGCTATGA478860.6847996668526701No Hit
TGTAAACATCCCCGACTGGAAGCT443290.6339323483254399No Hit
TTCAAGTAATTCAGGATAGGTT384450.5497874784310842No Hit
CAACGGAATCCCAAAAGCAGCTGT383080.5478282929831702No Hit
ACTGGACTTGGAGTCAGAAGGC327200.4679164077062057No Hit
CCCATAAAGTAGAAAGCACTAC314160.449268394391753No Hit
TATTGCACTTGTCCCGGCCTGT299530.4283465819078233No Hit
TATTGCACTTGTCCCGGCCTGTA297940.42607278273834637No Hit
CAACGGAATCCCAAAAGCAGCT275750.39433969873162045No Hit
TGTAAACATCCTACACTCTCAGCT265220.3792811419677258No Hit
CCCCCACTGCTAAATTTGACTGGCTT260690.3728029594282725No Hit
TTCTCACTACTGCACTTGACTAG254490.36393657272968305No Hit
CAAAGAATTCTCCTTTTGGGCT240830.3444019207453714No Hit
TCCTGTCTGAGCGTCGCT238650.34128438477715767No Hit
TAGCTTATCAGACTGATGTTGACA232580.33260390618676444No Hit
AACCGGGCGGAAACACCA223750.31997645545312814No Hit
CTTCTCACTACTGCACTTGACTAG221250.31640129952627755No Hit
TTAATGCTAATCGTGATAGGGGTT216330.30936539266223556No Hit
TGTCAGTTTGTCAAATACCCCA195630.2797631015879127No Hit
TGAGGTAGTAGTTTGTACAGTT193000.27600203755286584No Hit
ACCCCCCACTGCTAAATTTGACTG189890.2715545435798637No Hit
TGTCAGTTTGTCAAATACCCCAA182970.26165851197434126No Hit
CCCCCCACTGCTAAATTTGACTGG176970.25307813774989985No Hit
TAGCAGCACGTAAATATTGGC170970.24449776352545838No Hit
CATTGCACTTGTCTCGGTCTGA167060.2389062196558641No Hit
CAAAGAATTCTCCTTTTGGGCTT159950.22873847619990098No Hit
CCCCCCACTGCTAAATTTGACTGGCT156280.22349014729928432No Hit
CCCATAAAGTAGAAAGCACT139300.19920768824411508No Hit
AACATTCAACGCTGTCGGTGAGTT137540.1966907784716123No Hit
AACGGAATCCCAAAAGCAGCTG127370.18214704416118405No Hit
CACCCGGCTGTGTGCACATGTGC120580.17243692066385782No Hit
GGAATACCGGGTGCTGTAGGCTTT118500.16946239093271814No Hit
TGTAAACATCCCCGACTGGAAGC117670.16827543916500373No Hit
TTCAAGTAATCCAGGATAGGCTT117180.16757470860334103No Hit
ACTGGACTTGGAGTCAGAAGGCA105450.15080007699455805No Hit
ATCACATTGCCAGGGATTTCC104600.14958452397942884No Hit
TTATAAAGCAATGAGACTGATT103940.1486406828147403No Hit
TGAGGTAGTAGTTTGTGCTGTT103350.14779694601600354No Hit
CACTAGATTGTGAGCTCCTGGAA101380.14497972314564528No Hit
AGCTCGGTCTGAGGCCCCTCAGT96670.13824412937945874No Hit
TGTCAGTTTGTCAAATACCCC96420.1378866137867737No Hit
CTCCCACTGCTTCACTTGACTAG95830.13704287698803697No Hit
TTCACAGTGGCTAAGTTCTGC95750.13692847199837774No Hit
ATCACATTGCCAGGGATTTCCA93770.13409694850431206No Hit
CAACGGAATCCCAAAAGCAGCTGA92570.13238087365942378No Hit
TATTGCACTTGTCCCGGCCTGTAG90850.12992116638175058No Hit
TCAGTGCACTACAGAACTTTGTC90710.12972095764984692No Hit
CAGTGCAATAGTATTGTCAAAGC90400.12927763831491748No Hit
TCAGTGCACTACAGAACTTTGT90130.1288915214748176No Hit
TGAGAACTGAATTCCATAGGCTGT89730.1283194965265215No Hit
TAGCAGCACGTAAATATTGGCGT89610.12814788904203267No Hit
CACTAGATTGTGAGCTCCTGGA88070.1259455929910927No Hit
TGTAAACATCCTACACTCAGCT85950.12291386076512341No Hit
ATGCACCTGGGCAAGGATTCTGA85770.12265644953839017No Hit
ACTGGACTTGGAGTCAGAAGGCT84750.12119778592023514No Hit
AATGCACCCGGGCAAGGATTCT83420.11929580296715062No Hit
GGAATACCGGGTGCTGTAGGCTT81670.11679319381835519No Hit
AGCAGCATTGTACAGGGCTATGT81550.11662158633386638No Hit
CGCCTGTCTGAGCGTCGCT80130.11459089776741523No Hit
CAACACCAGTCGATGGGCTGT79320.11343254724711564No Hit
TTCAAGTAATCCAGGATAGGC78930.11287482292252693No Hit
TGTAAACATCCTTGACTGGAAGC77310.11055812188192776No Hit
TTCACAGTGGCTAAGTTCCG77250.11047231813968335No Hit
TATTGCACTTGTCCCGGCCTGTAAT73510.10512388487311486No Hit
TTCACAGTGGCTAAGTTCCGC72470.103636620007545No Hit
TATTGCACTTGTCCCGGCCTGTAAA71530.10229236137904918No Hit
CCCCCCACTGCTAAATTTGACTGTA71450.10217795638938997No Hit
CTCCCACTGCTTCACTTGACTAGCCT70690.1010911089876274No Hit
TATTGCACTTGTCCCGGCCTGTT70430.10071929277123493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)