FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10669316
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8093017.58531287291519No Hit
CAACGGAATCCCAAAAGCAGCTG6015065.637718481672114No Hit
AAGCTGCCAGTTGAAGAACTGT3511073.290810769875032No Hit
AACATTCAACGCTGTCGGTGAGT3497913.2784763334406817No Hit
TAGCAGCACGTAAATATTGGCG3491193.27217789781463No Hit
TTCAAGTAATCCAGGATAGGCT3390023.177354574557544No Hit
CCCCCACTGCTAAATTTGACTG2656732.490065904880875No Hit
TAGCTTATCAGACTGATGTTGAC2471912.316840179820337No Hit
TAGCTTATCAGACTGATGTTGACT2041691.9136090823441725No Hit
GCCTGTCTGAGCGTCGCT1919941.7994967999823044No Hit
AACATTCAACGCTGTCGGTGAG1662441.5581504943709605No Hit
TAGCTTATCAGACTGATGTTGA1422361.333131383492625No Hit
TATTGCACTTGTCCCGGCCTGTAA1192921.1180847956888709No Hit
TGAGGTAGTAGGTTGTATAGTT1164081.0910540094604004No Hit
TGTAAACATCCTTGACTGGAAGCT1146211.074305044484576No Hit
TATTGCACTTGTCCCGGCCTGTAT1068441.001413773853919No Hit
CATAAAGTAGAAAGCACTACT902340.8457336908945241No Hit
TATTGCACTTGTCCCGGCCTGT898760.8423782742961217No Hit
AGCAGCATTGTACAGGGCTATGA763930.7160065368764034No Hit
TCCTGTCTGAGCGTCGCT670050.6280158915529356No Hit
ACTGGACTTGGAGTCAGAAGGC651260.6104046407473543No Hit
TGTAAACATCCCCGACTGGAAGCT633830.5940680733422836No Hit
TGAGGTAGTAGATTGTATAGTT589670.552678353513946No Hit
TTCAAGTAATTCAGGATAGGTT566350.5308212822640177No Hit
TATTGCACTTGTCCCGGCCTGTA560420.5252632877309099No Hit
CGCCTGTCTGAGCGTCGCT546170.5119072300417384No Hit
GGAATACCGGGTGCTGTAGGCTTT472550.44290561831705044No Hit
CCCATAAAGTAGAAAGCACTAC464730.43557618876411575No Hit
CAACGGAATCCCAAAAGCAGCTGT434170.40693330294088204No Hit
AACCGGGCGGAAACACCA402550.3772969138790153No Hit
TGTAAACATCCTACACTCTCAGCT361310.33864401429295No Hit
TTCTCACTACTGCACTTGACTAG358870.3363570823096813No Hit
GGAATACCGGGTGCTGTAGGCTT341260.31985180680748415No Hit
AACATTCAACGCTGTCGGTGAGTT317510.2975917106588651No Hit
TAGCTTATCAGACTGATGTTGACA307980.28865955418322975No Hit
CATTGCACTTGTCTCGGTCTGA296990.2783589875864582No Hit
CCCCCACTGCTAAATTTGACTGGCTT290760.2725198128914731No Hit
CTTCTCACTACTGCACTTGACTAG278510.2610382896148169No Hit
TCGTACGACTCTTAGCGG269340.2524435493334343No Hit
TGAGGTAGTAGTTTGTACAGTT266680.249950418564789No Hit
CCCATAAAGTAGAAAGCACT240210.2251409556151491No Hit
CCCCCCACTGCTAAATTTGACTGGCT234520.2198079052115431No Hit
TGATGTAGTAGGTTGTATAGTT228850.21449360015206223No Hit
CGCGACCTCAGATCAGACGTGGCG222240.2082982639186992No Hit
CAACACCAGTCGATGGGCTGT220530.20669553699599863No Hit
CTACGCCTGTCTGAGCGTCGCT220430.20660181027537283No Hit
TTCAAGTAATCCAGGATAGGCTT215040.20154994003364415No Hit
CACCCGGCTGTGTGCACATGTGC211500.1982320141234921No Hit
TACGCCTGTCTGAGCGTCGCT211160.19791334327336446No Hit
CGCGACCTCAGATCAGACGTGG210660.19744470967023564No Hit
CAAAGAATTCTCCTTTTGGGCT209640.19648869711985287No Hit
CCCCCCACTGCTAAATTTGACTGG200020.1874721865956543No Hit
CAAAGAATTCTCCTTTTGGGCTT191390.17938357060565083No Hit
CTCCCACTGCTTCACTTGACTAG179230.16798640137755785No Hit
AGCTCGGTCTGAGGCCCCTCAGT177300.1661774756694806No Hit
TGAGGTAGTAGTTTGTGCTGTT176300.16524020846322296No Hit
TAGCAGCACGTAAATATTGGC174560.16360936352433464No Hit
ACCGGGTGCTGTAGGCTT171210.16046951838337153No Hit
TGAATACCGGGTGCTGTAGGCTTT169620.15897926352542188No Hit
CAACGGAATCCCAAAAGCAGCT163430.15317757951868705No Hit
AGCAGCATTGTACAGGGCTATGT162720.15251211980224413No Hit
TGTAAACATCCCCGACTGGAAGC160440.15037515057197667No Hit
TATTGCACTTGTCCCGGCCTGTT160060.15001898903359878No Hit
TTATAAAGCAATGAGACTGATT154340.14465782061380505No Hit
TGTAAACATCCTACACTCAGCT151030.14155546616109224No Hit
ACTTGACTTGGAGTCAGAAGGC150890.14142424875221618No Hit
ACGCCTGTCTGAGCGTCGCT150190.1407681617078358No Hit
TTCACAGTGGCTAAGTTCCG148700.1393716335705119No Hit
TAGCAGCACGTAAATATTGGCGT145480.13635363316636231No Hit
CACTAGATTGTGAGCTCCTGGA145390.13626927911779912No Hit
TTCAAGTAATCCAGGATAGGC144800.13571629146610711No Hit
ACTGGACTTGGAGTCAGAAGGCA142850.1338886204139047No Hit
GTCAGGATGGCCGAGCGGTCTAAGG140560.1317422785115747No Hit
TATTGCACTTGTCCCGGCCTGTAG140540.13172353316744953No Hit
ACTGGACTTGGAGTCAGAAGG132500.12418790482913807No Hit
CTCCCACTGCTTCACTTGACTAGCCT131520.12326938296700557No Hit
CACTAGATTGTGAGCTCCTGGAA131110.12288510341243992No Hit
AACGGAATCCCAAAAGCAGCTG129790.12164791070017984No Hit
ACCGGGTGCTGTAGGCTTT126310.11838622082240323No Hit
ACCCCCCACTGCTAAATTTGACTG124850.11701781070126707No Hit
ACCACAGGGTAGAACCACGGAC123780.11601493479057139No Hit
CAAAGTGCTGTTCGTGCAGGTAG123670.11591183539788304No Hit
ACTGGACTTGGAGTCAGAAGGCT121920.11427161778693216No Hit
TGAATACCGGGTGCTGTAGGCTT121700.11406541900155548No Hit
TGTCAGTTTGTCAAATACCCCA121100.1135030586778009No Hit
GCGTTCGCGCTTTCCCCTG120300.11275324491279479No Hit
TGATGTAGTAGATTGTATAGTT114630.10743893985331393No Hit
AGCAGCATTGTACAGGGCTATG113090.10599554835567716No Hit
TGTCAGTTTGTCAAATACCCCAA111000.10403665989459868No Hit
ACCGGGTGCTGTAGGCTTA109660.10278072183821343No Hit
GACCCGCGGGCGCTCTCCAGTCCT108260.10146854774945274No Hit
TTCACAGTGGCTAAGTTCCGC107880.10111238621107482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)