FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12207598
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG168431413.797259706618782No Hit
CAACGGAATCCCAAAAGCAGCTG8742447.16147435392286No Hit
CCCCCACTGCTAAATTTGACTG5784344.738311336923119No Hit
AACATTCAACGCTGTCGGTGAGT3625422.9698061813634427No Hit
TTCAAGTAATCCAGGATAGGCT2812982.3042862322301243No Hit
TAGCTTATCAGACTGATGTTGAC2480812.0321852013803205No Hit
AACATTCAACGCTGTCGGTGAG2465152.019357124964305No Hit
TAGCAGCACGTAAATATTGGCG2409051.9734021385697662No Hit
TAGCTTATCAGACTGATGTTGACT2215071.8145011000526063No Hit
GGAATACCGGGTGCTGTAGGCTTT2135821.749582514103102No Hit
TATTGCACTTGTCCCGGCCTGTAA2086261.7089848469780868No Hit
AAGCTGCCAGTTGAAGAACTGT1884411.5436370037742069No Hit
GCCTGTCTGAGCGTCGCT1865731.5283350582153834No Hit
TGAGGTAGTAGGTTGTATAGTT1835061.5032113606624333No Hit
TATTGCACTTGTCCCGGCCTGTAT1736531.4224993319734152No Hit
TAGCTTATCAGACTGATGTTGA1450161.1879159192496345No Hit
GGAATACCGGGTGCTGTAGGCTT1430001.171401613978442No Hit
TGAGGTAGTAGATTGTATAGTT1072430.8784938691460843No Hit
TATTGCACTTGTCCCGGCCTGT942590.772133879244713No Hit
TATTGCACTTGTCCCGGCCTGTA861090.7053721788676199No Hit
ACTGGACTTGGAGTCAGAAGGC850210.69645969665777No Hit
AGCAGCATTGTACAGGGCTATGA811820.6650120687132719No Hit
TGTAAACATCCTTGACTGGAAGCT791710.6485387215404701No Hit
TGTAAACATCCCCGACTGGAAGCT741020.6070154013918216No Hit
CACCCGGCTGTGTGCACATGTGC567100.464546751949073No Hit
TTCAAGTAATTCAGGATAGGTT563130.4612946789368392No Hit
CATAAAGTAGAAAGCACTACT525090.430133757681077No Hit
CATTGCACTTGTCTCGGTCTGA491920.402962155208584No Hit
CAACGGAATCCCAAAAGCAGCTGT476800.3905764262551896No Hit
CAACGGAATCCCAAAAGCAGCT462760.3790753922270376No Hit
CCCCCACTGCTAAATTTGACTGGCTT447920.366919028624632No Hit
TTAATGCTAATCGTGATAGGGGTT417430.3419427802258888No Hit
TAGCTTATCAGACTGATGTTGACA373490.3059488033600058No Hit
TGAGGTAGTAGTTTGTACAGTT365590.2994774238142508No Hit
CGCGACCTCAGATCAGACGTGG342720.28074318961027384No Hit
GGAATACCGGGTGCTGTAGGCTTA317640.2601986074574212No Hit
CACTAGATTGTGAGCTCCTGGA316510.2592729544337879No Hit
TTCTCACTACTGCACTTGACTAG308080.25236741904508975No Hit
CAACACCAGTCGATGGGCTGT306380.2509748436998007No Hit
ACCGGGTGCTGTAGGCTT297510.24370887704526314No Hit
TGAGGTAGTAGTTTGTGCTGTT285370.23376425075596363No Hit
CACTAGATTGTGAGCTCCTGGAA280150.2294882252839584No Hit
CCCATAAAGTAGAAAGCACTAC278010.2277352186728298No Hit
CGCGACCTCAGATCAGACGTGGCG277710.22748947008248468No Hit
TGAGAACTGAATTCCATGGGTT260860.21368659092476672No Hit
ACTGGACTTGGAGTCAGAAGGCA257170.21066388326352162No Hit
AACATTCAACGCTGTCGGTGAGTT250390.20510996512172175No Hit
TATTGCACTTGTCCCGGCCTGTAG250300.2050362405446182No Hit
ACCGGGTGCTGTAGGCTTT247310.20258694626084506No Hit
ACTGGACTTGGAGTCAGAAGGCT246670.20206268260144217No Hit
ACCCCCCACTGCTAAATTTGACTG244880.20059638267904953No Hit
CAAAGAATTCTCCTTTTGGGCT240950.1973770761455284No Hit
CTTCTCACTACTGCACTTGACTAG239840.1964678063612514No Hit
TGAATACCGGGTGCTGTAGGCTTT236800.19397755397908745No Hit
ACCGGGTGCTGTAGGCTTA234390.19200337363664827No Hit
GGAATACCGGGTGCTGTAGGCT231670.18977525308418577No Hit
CCCATAAAGTAGAAAGCACT225460.18468825726404162No Hit
AACGGAATCCCAAAAGCAGCTG225340.18458995782790358No Hit
CCCCCCACTGCTAAATTTGACTGG219810.18005999214587506No Hit
CCCCCCACTGCTAAATTTGACTGGCT214310.17555460132288103No Hit
TATTGCACTTGTCCCGGCCTGTAAT200830.16451229799670664No Hit
TGTAAACATCCTACACTCTCAGCT193720.1586880564055271No Hit
TCCTGTCTGAGCGTCGCT190930.1564025945153174No Hit
TAGCAGCACGTAAATATTGGC180820.14812086702068664No Hit
TTATAAAGCAATGAGACTGATT180530.14788331005001967No Hit
CGCGACCTCAGATCAGACGTGGC176550.14462304541810764No Hit
TATTGCACTTGTCCCGGCCTGTT176050.14421346443419908No Hit
CAAAGAATTCTCCTTTTGGGCTT175930.14411516499806104No Hit
CTCCCACTGCTTCACTTGACTAG174890.14326323655153128No Hit
GACCCGCGGGCGCTCTCCAGTCCT165270.13538289842113085No Hit
CCCCCCACTGCTAAATTTGACTGTA165170.13530098222434914No Hit
AGCAGCATTGTACAGGGCTATGT162950.13348244265579517No Hit
CAACGGAATCCCAAAAGCAGCTGA162210.13287626279961054No Hit
ACTGGACTTGGAGTCAGAAGG161950.13266328068797809No Hit
CTCCCACTGCTTCACTTGACTAGCCT161920.13263870582894358No Hit
TGTAAACATCCCCGACTGGAAGC161240.13208167569082796No Hit
TGAATACCGGGTGCTGTAGGCTT160190.13122155562462No Hit
TTCAAGTAATCCAGGATAGGCTT154610.12665063184420064No Hit
TATTGCACTTGTCCCGGCCTGTAAA153960.12611817656511953No Hit
CCCCCCACTGCTAAATTTGACTGT150750.12348866664842666No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC150540.12331664263518508No Hit
TCAGTGCACTACAGAACTTTGT147960.12120320475821698No Hit
TCGTACGACTCTTAGCGG146550.12004818638359488No Hit
CGCCTGTCTGAGCGTCGCT145540.11922083279609962No Hit
AATGCACCCGGGCAAGGATTCT144310.11821326357568458No Hit
TGAGGTAGTAGGTTGTATAGT141460.11587865196740588No Hit
ATGCACCTGGGCAAGGATTCTGA134440.11012813495332988No Hit
AGCTCGGTCTGAGGCCCCTCAGT133260.10916152383130572No Hit
TGAGGTAGTAGGTTGTGTGGTT131030.1073347926430736No Hit
AGCAGCATTGTACAGGGCTATG127000.10403356991277073No Hit
AACCGGGCGGAAACACCA126390.1035338811124023No Hit
TGAGGTAGTAAGTTGTATTGTT123180.10090437119570943No Hit
CAAAGTGCTGTTCGTGCAGGTAG122330.1002080835230649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)