FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8398769
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG107491812.798518449548975No Hit
CAACGGAATCCCAAAAGCAGCTG6827008.128572175279496No Hit
CCCCCACTGCTAAATTTGACTG3175923.781411299679751No Hit
TTCAAGTAATCCAGGATAGGCT2385442.8402257521310563No Hit
TAGCAGCACGTAAATATTGGCG2136782.5441585546643797No Hit
AACATTCAACGCTGTCGGTGAGT1936442.3056235979344115No Hit
AAGCTGCCAGTTGAAGAACTGT1791322.132836371615888No Hit
TATTGCACTTGTCCCGGCCTGTAT1696422.0198436223213188No Hit
AACATTCAACGCTGTCGGTGAG1544721.8392219145448578No Hit
TATTGCACTTGTCCCGGCCTGTAA1473781.754757155483143No Hit
TAGCTTATCAGACTGATGTTGACT1408681.6772457963780167No Hit
TAGCTTATCAGACTGATGTTGAC1258541.4984815036584527No Hit
TGAGGTAGTAGGTTGTATAGTT933251.1111747447750973No Hit
TATTGCACTTGTCCCGGCCTGT895791.0665729703960187No Hit
TAGCTTATCAGACTGATGTTGA866721.0319607552011492No Hit
TATTGCACTTGTCCCGGCCTGTA722330.8600427038771992No Hit
GCCTGTCTGAGCGTCGCT697310.8302526239261967No Hit
TGTAAACATCCTTGACTGGAAGCT663430.7899133789725614No Hit
TTCAAGTAATTCAGGATAGGTT590070.7025672452713011No Hit
TGAGGTAGTAGATTGTATAGTT554220.6598824184829943No Hit
AGCAGCATTGTACAGGGCTATGA488530.5816685754781445No Hit
TGTAAACATCCCCGACTGGAAGCT455070.5418294038090582No Hit
CATAAAGTAGAAAGCACTACT424270.5051573629421169No Hit
CAACGGAATCCCAAAAGCAGCTGT387510.4613890440372869No Hit
ACTGGACTTGGAGTCAGAAGGC338920.4035353276176544No Hit
CACCCGGCTGTGTGCACATGTGC292010.3476819043362188No Hit
CCCATAAAGTAGAAAGCACTAC281430.33508482016828894No Hit
GGAATACCGGGTGCTGTAGGCTTT277870.3308461037563957No Hit
CATTGCACTTGTCTCGGTCTGA277570.33048890855314633No Hit
TGAGAACTGAATTCCATGGGTT273820.32602396851252846No Hit
TTCTCACTACTGCACTTGACTAG259180.30859284259395636No Hit
CCCCCACTGCTAAATTTGACTGGCTT249560.29713878307642466No Hit
CTCCCACTGCTTCACTTGACTAG241190.28717303690576557No Hit
TGATGTAGTAGGTTGTATAGTT237190.2824104341957732No Hit
CACTAGATTGTGAGCTCCTGGAA233010.2774335143638312No Hit
CACTAGATTGTGAGCTCCTGGA230980.27501649348851004No Hit
TCGTACGACTCTTAGCGG227930.27138500892214085No Hit
ACCGGGTGCTGTAGGCTTA227290.27062299248854205No Hit
TCCTGTCTGAGCGTCGCT223400.2659913613530745No Hit
TATTGCACTTGTCCCGGCCTGTAG215500.2565852210008395No Hit
CAACGGAATCCCAAAAGCAGCT202940.24163064849146346No Hit
GGAATACCGGGTGCTGTAGGCTT200570.238808806385793No Hit
TATTGCACTTGTCCCGGCCTGTT197540.23520113483297372No Hit
CCCATAAAGTAGAAAGCACT195030.23221260163245352No Hit
AGCTCGGTCTGAGGCCCCTCAGT192260.22891449925578378No Hit
TAGCTTATCAGACTGATGTTGACA191890.2284739585051095No Hit
TGTAAACATCCTACACTCTCAGCT191410.22790244617991043No Hit
TGAGGTAGTAGTTTGTACAGTT190300.2265808239278875No Hit
CAAAGAATTCTCCTTTTGGGCT188000.2238423273696419No Hit
ACCCCCCACTGCTAAATTTGACTG186300.22181822121789516No Hit
ACCGGGTGCTGTAGGCTT181950.21663889077077844No Hit
TTAATGCTAATCGTGATAGGGGTT180970.21547205310683026No Hit
TGAGAACTGAATTCCATAGGCTGT179040.21317409729925899No Hit
CTTCTCACTACTGCACTTGACTAG170090.202517773735651No Hit
CAACACCAGTCGATGGGCTGT167890.19989834224515524No Hit
TATTGCACTTGTCCCGGCCTGTTT165930.19756466691725894No Hit
CCCCCCACTGCTAAATTTGACTGGCT165230.19673121144301026No Hit
ACCGGGTGCTGTAGGCTTT158390.18858716080892332No Hit
CCCCCCACTGCTAAATTTGACTGG157100.18705122143495076No Hit
AACCGGGCGGAAACACCA154470.18391981015313077No Hit
TGATGTAGTAGATTGTATAGTT142270.169393871887654No Hit
TAGCAGCACGTAAATATTGGC141760.16878664004212998No Hit
TATTGCACTTGTCCCGGCCTGTAAT137450.1636549356221132No Hit
AACGGAATCCCAAAAGCAGCTG134450.16008298358961892No Hit
CTCCCACTGCTTCACTTGACTAGCCT130830.1557728281370758No Hit
TTCAAGTAATCCAGGATAGGCTT126890.1510816644677333No Hit
AACATTCAACGCTGTCGGTGAGTT126030.15005770488508494No Hit
TGTAAACATCCCCGACTGGAAGC119610.14241372753554718No Hit
GACCCGCGGGCGCTCTCCAGTCCT119110.14181840219679814No Hit
TGAGGTAGTAGTTTGTGCTGTT116610.13884177550305288No Hit
ATCCCGGACGAGCCCCCA116360.13854411283367835No Hit
CCCCCCACTGCTAAATTTGACTGT115630.13767493783910476No Hit
TATTGCACTTGTCCCGGCCTGTAAA114480.13630568955998193No Hit
CAAAGAATTCTCCTTTTGGGCTT114390.1361985309990071No Hit
CGCGACCTCAGATCAGACGTGG111110.13229319677681337No Hit
AGCAGCATTGTACAGGGCTATGT107810.12836404954106967No Hit
TGAATACCGGGTGCTGTAGGCTTT107690.12822117145976988No Hit
TTCAAGTAATCCAGGATAGGC106640.1269709882483969No Hit
CGCGACCTCAGATCAGACGTGGCG105640.1257803375708988No Hit
CCCCCCACTGCTAAATTTGACTGA98440.11720765269291249No Hit
ACTTGACTTGGAGTCAGAAGGC89160.10615841440573016No Hit
TCGTTTCCCGGGCGGCGCACCA88840.10577740618893078No Hit
ACTGGACTTGGAGTCAGAAGGCA88630.10552736954665617No Hit
CAAAGTGCTGTTCGTGCAGGTAG88620.10551546303988119No Hit
TATTGCACTTGTCCCGGCCTGA87750.10447959695045787No Hit
TGAGAACTGAATTCCATGGGTTG86200.1026340884003358No Hit
TCAGTGCACTACAGAACTTTGT85300.10156250279058754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)