FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691598
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG114646414.905407172865766No Hit
CAACGGAATCCCAAAAGCAGCTG5655047.352230316769025No Hit
CCCCCACTGCTAAATTTGACTG3274754.257567803205524No Hit
TTCAAGTAATCCAGGATAGGCT1816662.361875906671149No Hit
TAGCAGCACGTAAATATTGGCG1685612.1914951873459843No Hit
AACATTCAACGCTGTCGGTGAGT1594002.0723911988120025No Hit
TATTGCACTTGTCCCGGCCTGTAT1492331.9402080035904112No Hit
AACATTCAACGCTGTCGGTGAG1411801.8355093440920858No Hit
TATTGCACTTGTCCCGGCCTGTAA1394871.8134983133543903No Hit
AAGCTGCCAGTTGAAGAACTGT1268431.6491111469944217No Hit
TAGCTTATCAGACTGATGTTGACT1106431.4384917152456485No Hit
TAGCTTATCAGACTGATGTTGAC1008181.310754930249865No Hit
GCCTGTCTGAGCGTCGCT830961.0803476728762995No Hit
TATTGCACTTGTCCCGGCCTGT723960.9412348383261839No Hit
GGAATACCGGGTGCTGTAGGCTTT701190.9116311070859396No Hit
TAGCTTATCAGACTGATGTTGA690100.8972127768507923No Hit
TATTGCACTTGTCCCGGCCTGTA675190.8778279884102107No Hit
TGAGGTAGTAGGTTGTATAGTT610820.7941392672888001No Hit
TGTAAACATCCTTGACTGGAAGCT539300.7011546885315639No Hit
GGAATACCGGGTGCTGTAGGCTT510270.6634122064101634No Hit
AGCAGCATTGTACAGGGCTATGA444480.5778773149610783No Hit
TTCAAGTAATTCAGGATAGGTT439490.5713897164152364No Hit
CCCCCACTGCTAAATTTGACTGGCTT408120.5306049536130204No Hit
TGTAAACATCCCCGACTGGAAGCT403610.5247414126427304No Hit
TGAGGTAGTAGATTGTATAGTT363750.47291863147293967No Hit
ACTGGACTTGGAGTCAGAAGGC312490.4062744828837909No Hit
CATAAAGTAGAAAGCACTACT298770.38843683718259847No Hit
TCCTGTCTGAGCGTCGCT296740.3857975936859935No Hit
CAACGGAATCCCAAAAGCAGCTGT295520.38421144734813234No Hit
TGAATACCGGGTGCTGTAGGCTTT293520.3816112074499993No Hit
TTCTCACTACTGCACTTGACTAG278870.3625644501961751No Hit
CTCCCACTGCTTCACTTGACTAG252740.32859231592706745No Hit
CACCCGGCTGTGTGCACATGTGC252580.3283842967352168No Hit
AACCGGGCGGAAACACCA230180.29926160987612715No Hit
CCCCCCACTGCTAAATTTGACTGGCT229160.29793548752807936No Hit
CACTAGATTGTGAGCTCCTGGAA212780.2766395227623701No Hit
CATTGCACTTGTCTCGGTCTGA212690.2765225119669541No Hit
TGAATACCGGGTGCTGTAGGCTT212550.27634049517408477No Hit
CCCATAAAGTAGAAAGCACTAC205870.26765569391432054No Hit
TATTGCACTTGTCCCGGCCTGTAG203640.26475642642790226No Hit
CTTCTCACTACTGCACTTGACTAG188880.2455666559796807No Hit
ACCCCCCACTGCTAAATTTGACTG188550.24513761639648876No Hit
CAAAGAATTCTCCTTTTGGGCT185140.240704207370172No Hit
CCCCCCACTGCTAAATTTGACTGG184620.24002814499665737No Hit
CACTAGATTGTGAGCTCCTGGA182440.23719388350769244No Hit
CAACGGAATCCCAAAAGCAGCT178660.23227943010022106No Hit
TAGCTTATCAGACTGATGTTGACA175180.22775501267746964No Hit
TCGTACGACTCTTAGCGG173480.22554480876405655No Hit
TATTGCACTTGTCCCGGCCTGTT173020.224946753587486No Hit
TGATGTAGTAGGTTGTATAGTT170850.22212549329801165No Hit
ACCGGGTGCTGTAGGCTTA161750.21029440176150652No Hit
AGCTCGGTCTGAGGCCCCTCAGT156960.20406682720547795No Hit
GACCCGCGGGCGCTCTCCAGTCCT149210.1939908976002126No Hit
TATTGCACTTGTCCCGGCCTGTTT147440.1916896852903649No Hit
CTCCCACTGCTTCACTTGACTAGCCT140580.1827708624397687No Hit
TGTAAACATCCTACACTCTCAGCT134690.175113155939767No Hit
CCCATAAAGTAGAAAGCACT134320.17463211155861239No Hit
TAGCAGCACGTAAATATTGGC130760.17000368453993564No Hit
GGAATACCGGGTGCTGTAGGCTTA129840.16880757418679448No Hit
ACCGGGTGCTGTAGGCTT124050.16127987968169943No Hit
CAACACCAGTCGATGGGCTGT124020.16124087608322743No Hit
TGAGGTAGTAGTTTGTACAGTT123000.1599147537351796No Hit
TATTGCACTTGTCCCGGCCTGTAAA122770.15961572614689432No Hit
TATTGCACTTGTCCCGGCCTGTAAT117840.15320613479799647No Hit
AACATTCAACGCTGTCGGTGAGTT116200.1510739380815274No Hit
TGTAAACATCCCCGACTGGAAGC115820.15057989250088213No Hit
AACGGAATCCCAAAAGCAGCTG109860.1428311776044458No Hit
CAAAGAATTCTCCTTTTGGGCTT103820.13497845311208412No Hit
CCCCCCACTGCTAAATTTGACTGA102640.13344431157218564No Hit
ACCGGGTGCTGTAGGCTTT101960.13256023000682043No Hit
TGATGTAGTAGATTGTATAGTT101690.13220919762057248No Hit
CGCGACCTCAGATCAGACGTGGCG99650.1295569529244768No Hit
TTCAAGTAATCCAGGATAGGCTT99220.1289979013463782No Hit
GGAATACCGGGTGCTGTAGGCT98850.1285168569652236No Hit
AGCAGCATTGTACAGGGCTATGT97630.12693071062736247No Hit
ACTGGACTTGGAGTCAGAAGGCA96620.1256175894788053No Hit
TCGTTTCCCGGCCAATGCACCA91250.1186359453523182No Hit
TTCAAGTAATCCAGGATAGGC89470.11632173184297984No Hit
TCGTTTCCCGGGCGGCGCACCA89280.11607470905265721No Hit
ACTTGACTTGGAGTCAGAAGGC88990.11569767426742791No Hit
TGAGGTAGTAGTTTGTGCTGTT88900.11558066347201193No Hit
ACTGGACTTGGAGTCAGAAGGCT81380.10580376145503184No Hit
CCCCCCACTGCTAAATTTGACTGT80710.10493268108915728No Hit
ATCCCGGACGAGCCCCCA80580.10476366549577863No Hit
CCCCCACAACCGCGCTTGACTAG80190.10425661871564271No Hit
CCCCCACTGCTAAATTTGACTGGCT78510.10207241720121098No Hit
TATTGCACTTGTCCCGGCCTGA78470.10202041240324833No Hit
TTCACAGTGGCTAAGTTCCG77290.10048627086334985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)