FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11071603
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG10339519.338765127326187No Hit
GGAATACCGGGTGCTGTAGGCTTT8434297.617948367548945No Hit
CAACGGAATCCCAAAAGCAGCTG7488006.763248284823796No Hit
GGAATACCGGGTGCTGTAGGCTT5522434.987922706404845No Hit
CCCCCACTGCTAAATTTGACTG4766634.305275396886973No Hit
TGAATACCGGGTGCTGTAGGCTTT4265003.8521973737678272No Hit
TGAATACCGGGTGCTGTAGGCTT2773822.5053463351241914No Hit
AACATTCAACGCTGTCGGTGAGT2739112.4739958613039144No Hit
AACATTCAACGCTGTCGGTGAG2090681.8883263787547295No Hit
TTCAAGTAATCCAGGATAGGCT1974551.7834364183759117No Hit
TAGCAGCACGTAAATATTGGCG1777051.605052132017378No Hit
TAGCTTATCAGACTGATGTTGAC1753011.5833389257183446No Hit
AAGCTGCCAGTTGAAGAACTGT1742641.5739726216700507No Hit
TAGCTTATCAGACTGATGTTGACT1474201.331514506074685No Hit
TAGCTTATCAGACTGATGTTGA1351231.2204465785126146No Hit
TATTGCACTTGTCCCGGCCTGTAA1185211.0704953925822664No Hit
GGAATACCGGGTGCTGTAGGCTTA1137851.0277192923192784No Hit
TGAGGTAGTAGGTTGTATAGTT1038390.9378858689206973No Hit
TATTGCACTTGTCCCGGCCTGTAT1031650.9317982228950947No Hit
GGAATACCGGGTGCTGTAGGCT830630.750234631787285No Hit
TATTGCACTTGTCCCGGCCTGT764560.6905594429280024No Hit
TATTGCACTTGTCCCGGCCTGTA610330.5512571214845764No Hit
AGCAGCATTGTACAGGGCTATGA606940.5481952342402451No Hit
TGAATACCGGGTGCTGTAGGCTTA584100.52756588183301No Hit
TGAGGTAGTAGATTGTATAGTT539940.48768005861481845No Hit
CATAAAGTAGAAAGCACTACT511650.4621282031156645No Hit
CAACACCAGTCGATGGGCTGT504780.455923139585117No Hit
CATTGCACTTGTCTCGGTCTGA483980.4371363387939398No Hit
ACTGGACTTGGAGTCAGAAGGC447730.4043949191458545No Hit
TGAATACCGGGTGCTGTAGGCT424800.3836842776967346No Hit
TGTAAACATCCTTGACTGGAAGCT411020.3712380221725797No Hit
CACCCGGCTGTGTGCACATGTGC395600.35731049966296663No Hit
TTCAAGTAATTCAGGATAGGTT378850.34218170575660994No Hit
CAACGGAATCCCAAAAGCAGCT374650.3383882171353146No Hit
ACCGGGTGCTGTAGGCTT370020.3342063475361246No Hit
GCCTGTCTGAGCGTCGCT333760.3014558957722744No Hit
TCGTACGACTCTTAGCGG330910.2988817427792525No Hit
CAACGGAATCCCAAAAGCAGCTGT330510.2985204581486529No Hit
ACCGGGTGCTGTAGGCTTT327430.29573856649303626No Hit
TGTAAACATCCCCGACTGGAAGCT327300.29562114898809144No Hit
CCCATAAAGTAGAAAGCACT275350.24869930758897335No Hit
ACCGGGTGCTGTAGGCTTA268410.24243101924807095No Hit
TTAATGCTAATCGTGATAGGGGTT240550.21726754472681148No Hit
TAGCTTATCAGACTGATGTTGACA222650.20110005750748108No Hit
CACTAGATTGTGAGCTCCTGGA218090.19698141271864608No Hit
GGAATACCGGGTGCTGTAGGCTA217920.19682786675064126No Hit
TGATGTAGTAGGTTGTATAGTT215430.194578869925159No Hit
CCCATAAAGTAGAAAGCACTAC201150.18168100861275463No Hit
TGAGGTAGTAGTTTGTACAGTT193460.17473531158947805No Hit
AACGGAATCCCAAAAGCAGCTG190030.17163729588208682No Hit
GCCCCCACTGCTAAATTTGACTG179010.1616839043090689No Hit
CGCCTGTCTGAGCGTCGCT178300.16104262408975467No Hit
TTCTCACTACTGCACTTGACTAG175190.15823363608684307No Hit
CCCCCACTGCTAAATTTGACTGGCTT167740.15150470984192624No Hit
AACATTCAACGCTGTCGGTGAGTT161250.14564286671044835No Hit
GTAATACCGGGTGCTGTAGGCTTT161240.14563383459468338No Hit
TATTGCACTTGTCCCGGCCTGTAG157480.14223775906704747No Hit
TGAGGTAGTAGTTTGTGCTGTT157440.14220163060398752No Hit
CACTAGATTGTGAGCTCCTGGAA155870.14078358842888422No Hit
TCCTGTCTGAGCGTCGCT151190.13655655825086938No Hit
TGTAAACATCCTACACTCTCAGCT146310.1321488857575547No Hit
TAGCAGCACGTAAATATTGGC146030.13189598651613502No Hit
ATACCGGGTGCTGTAGGCTTA142200.12843668617814422No Hit
ATACCGGGTGCTGTAGGCTTT140400.12681090534044617No Hit
CTTCTCACTACTGCACTTGACTAG138530.12512189969239323No Hit
TTATAAAGCAATGAGACTGATT138310.12492319314556348No Hit
ATCCCGGACGAGCCCCCA132670.11982907985410966No Hit
ACCACAGGGTAGAACCACGGAC131670.11892586827761074No Hit
ACCCCCCACTGCTAAATTTGACTG131260.1185555515312462No Hit
AGCAGCATTGTACAGGGCTATGT130720.11806781727993679No Hit
TATTGCACTTGTCCCGGCCTGTT126430.11419303961675649No Hit
TCGTTTCCCGGCCAATGCACCA125870.1136872411339171No Hit
TTAATACCGGGTGCTGTAGGCTTT125840.11366014478662215No Hit
ACTGGACTTGGAGTCAGAAGGCT124930.11283822225200814No Hit
ATACCGGGTGCTGTAGGCTT120070.10844861399022344No Hit
AGCAGCATTGTACAGGGCTATG117910.10649767698498581No Hit
CCCGCCACTGCTAAATTTGACTG116620.10533253405130225No Hit
CGAATACCGGGTGCTGTAGGCTTT116380.10511576327294249No Hit
CTCCCACTGCTTCACTTGACTAG113770.10275838105828036No Hit
TGATGTAGTAGATTGTATAGTT112330.10145775638812193No Hit
CCCCCCACTGCTAAATTTGACTGG111890.10106034329446242No Hit
CGCGACCTCAGATCAGACGTGG111740.10092486155798758No Hit
TGAATACCGGGTGCTGTAGGCTA111590.10078937982151275No Hit
GAATACCGGGTGCTGTAGGCTTT111010.10026551710714338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)