FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8385173
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG96970511.564519897204267No Hit
CAACGGAATCCCAAAAGCAGCTG6683187.970235080421119No Hit
AACATTCAACGCTGTCGGTGAGT3453294.11832886453267No Hit
CCCCCACTGCTAAATTTGACTG3291343.9251903329841853No Hit
TAGCAGCACGTAAATATTGGCG2769753.30315188488061No Hit
AAGCTGCCAGTTGAAGAACTGT2726033.2510122331405684No Hit
TTCAAGTAATCCAGGATAGGCT2446972.9182105127705773No Hit
AACATTCAACGCTGTCGGTGAG2089492.4918865716902916No Hit
TAGCTTATCAGACTGATGTTGACT1394371.6628995012983034No Hit
TAGCTTATCAGACTGATGTTGAC1371651.6358040555633142No Hit
TATTGCACTTGTCCCGGCCTGTAA1335611.5928234277336912No Hit
TATTGCACTTGTCCCGGCCTGTAT1249661.4903210702987284No Hit
TAGCTTATCAGACTGATGTTGA1210771.4439415859398488No Hit
TATTGCACTTGTCCCGGCCTGT862141.0281719888188352No Hit
TGAGGTAGTAGGTTGTATAGTT750860.8954615486168265No Hit
TGTAAACATCCTTGACTGGAAGCT693390.8269239048496673No Hit
CATAAAGTAGAAAGCACTACT637960.7608191268087134No Hit
TATTGCACTTGTCCCGGCCTGTA555530.6625146553326926No Hit
TTCAAGTAATTCAGGATAGGTT508270.6061532660089421No Hit
AGCAGCATTGTACAGGGCTATGA503780.6007985762488144No Hit
TGAGGTAGTAGATTGTATAGTT478220.570316199796951No Hit
TGTAAACATCCCCGACTGGAAGCT466830.5567326994923062No Hit
TTAATGCTAATCGTGATAGGGGTT405040.4830431047755365No Hit
TTCTCACTACTGCACTTGACTAG393160.46887523966410705No Hit
CAACGGAATCCCAAAAGCAGCTGT363820.43388490613133446No Hit
CACCCGGCTGTGTGCACATGTGC348320.4153998969371294No Hit
ACTGGACTTGGAGTCAGAAGGC332220.3961993389999229No Hit
CTTCTCACTACTGCACTTGACTAG292540.34887771546275786No Hit
CATTGCACTTGTCTCGGTCTGA289350.3450733813124667No Hit
CCCATAAAGTAGAAAGCACT282010.3363198350230818No Hit
CAACACCAGTCGATGGGCTGT279400.33320719799102533No Hit
CCCATAAAGTAGAAAGCACTAC267260.3187292617576286No Hit
ACCGGGTGCTGTAGGCTT254740.3037981446536643No Hit
CCCCCCACTGCTAAATTTGACTGG238970.28499113852510854No Hit
CGCCTGTCTGAGCGTCGCT235020.28028044263368207No Hit
TGAGAACTGAATTCCATAGGCTGT231720.2763449245471739No Hit
ACCGGGTGCTGTAGGCTTT228950.27304147451698374No Hit
ACCGGGTGCTGTAGGCTTA223740.26682812626525415No Hit
TGTAAACATCCTACACTCTCAGCT215950.2575379184186182No Hit
TGAGAACTGAATTCCATGGGTT211700.252469448155691No Hit
TGATGTAGTAGGTTGTATAGTT206930.24678083564882916No Hit
TGAGGTAGTAGTTTGTACAGTT205420.2449800379789421No Hit
AACATTCAACGCTGTCGGTGAGTT198410.2366200434982081No Hit
TAGCTTATCAGACTGATGTTGACA189540.22604184791416942No Hit
CAACGGAATCCCAAAAGCAGCT188150.22438415999288266No Hit
CCCCCACTGCTAAATTTGACTGGCTT187390.2234777982517475No Hit
CAAAGAATTCTCCTTTTGGGCT173040.20636425748162857No Hit
TAGCAGCACGTAAATATTGGC163590.19509436477935518No Hit
TATTGCACTTGTCCCGGCCTGTAG159840.1906221851355959No Hit
TGTAAACATCCCCGACTGGAAGC158660.18921493927435964No Hit
CAAAGAATTCTCCTTTTGGGCTT157740.1881177645350907No Hit
ACCCCCCACTGCTAAATTTGACTG153970.18362173326656467No Hit
CTCCCACTGCTTCACTTGACTAG153580.1831566265836137No Hit
CACTAGATTGTGAGCTCCTGGA149620.1784340048798039No Hit
AACGGAATCCCAAAAGCAGCTG148030.17653780071084998No Hit
TATTGCACTTGTCCCGGCCTGTT143560.1712069625754889No Hit
AGCTCGGTCTGAGGCCCCTCAGT142780.17027674920958696No Hit
ATCCCGGACGAGCCCCCA141900.16922727771985147No Hit
TTCAAGTAATCCAGGATAGGCTT137360.1638129588978069No Hit
TGAGGTAGTAGTTTGTGCTGTT134080.1599012924360654No Hit
CACTAGATTGTGAGCTCCTGGAA133630.1593646308788143No Hit
GACCCGCGGGCGCTCTCCAGTCCT131510.1568363586535424No Hit
TGATGTAGTAGATTGTATAGTT130990.15621621640960776No Hit
AACATTCAACGCTGTCGGTGA125540.1497166486606776No Hit
CCCCCCACTGCTAAATTTGACTGGCT118630.14147591230377715No Hit
TATTGCACTTGTCCCGGCCTGTAAT107670.1284052219316167No Hit
TGTCAGTTTGTCAAATACCCCAA106950.1275465634400149No Hit
TGTCAGTTTGTCAAATACCCCA106380.12686679213416346No Hit
TTCAAGTAATCCAGGATAGGC104370.12446970384510851No Hit
CCCCCCACTGCTAAATTTGACTGT103740.12371837766495695No Hit
CTACGCCTGTCTGAGCGTCGCT97470.1162408933005914No Hit
CAAAGTGCTGTTCGTGCAGGT95870.11433276331925411No Hit
GGAATACCGGGTGCTGTAGGCTTT95450.11383187919915307No Hit
TATTGCACTTGTCCCGGCCTGTTT93470.11147056834724817No Hit
ACTGGACTTGGAGTCAGAAGGCT93460.11145864253486482No Hit
AGCAGCATTGTACAGGGCTATGT92090.10982480623834476No Hit
AATGACACGATCACTCCCGTTG90940.10845333781425857No Hit
ACCACAGGGTAGAACCACGGAC90810.10829830225327491No Hit
TCGTACGACTCTTAGCGG90280.10766623419695694No Hit
AACATTCAACGCTGTCGGTGAGA89720.10699838870348889No Hit
ACTTGACTTGGAGTCAGAAGGC89090.10624706252333732No Hit
TGAGGTAGTAGGTTGTGTGGTT89020.10616358183665382No Hit
TTCACAGTGGCTAAGTTCTGC88450.1054838105308024No Hit
CCCCCACTGCTAAATTTGACTGG86380.10301516736744727No Hit
TATTGCACTTGTCCCGGCCTGTAAA84170.10037956283072513No Hit
TTCACAGTGGCTAAGTTCCG84150.10035571120595843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)