FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526666
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG95166611.161056384758123No Hit
CAACGGAATCCCAAAAGCAGCTG7167638.406134355444438No Hit
CCCCCACTGCTAAATTTGACTG3577734.195930742449628No Hit
AACATTCAACGCTGTCGGTGAGT2287722.6830181925737446No Hit
TTCAAGTAATCCAGGATAGGCT2188062.5661378081421273No Hit
AAGCTGCCAGTTGAAGAACTGT2170462.545496680648685No Hit
TAGCAGCACGTAAATATTGGCG2079082.438327008469664No Hit
AACATTCAACGCTGTCGGTGAG2034822.3864192639889965No Hit
TATTGCACTTGTCCCGGCCTGTAT1522651.785750726016476No Hit
TATTGCACTTGTCCCGGCCTGTAA1305001.530492691985355No Hit
TAGCTTATCAGACTGATGTTGACT1253811.470457503554144No Hit
TAGCTTATCAGACTGATGTTGAC1178581.3822284114330265No Hit
TATTGCACTTGTCCCGGCCTGT1138321.3350118322917774No Hit
TAGCTTATCAGACTGATGTTGA1114511.3070876706088874No Hit
TATTGCACTTGTCCCGGCCTGTA924411.0841400378530133No Hit
CATAAAGTAGAAAGCACTACT731390.8577678543993631No Hit
TGAGGTAGTAGGTTGTATAGTT645900.7575059231826367No Hit
ATCCCGGACGAGCCCCCA557130.6533972363875868No Hit
TGTAAACATCCTTGACTGGAAGCT530940.6226818313277429No Hit
AGCAGCATTGTACAGGGCTATGA484050.567689645636407No Hit
TTAATGCTAATCGTGATAGGGGTT476940.5593510992455902No Hit
ACCGGGTGCTGTAGGCTTA471630.5531235772575119No Hit
ACCGGGTGCTGTAGGCTT409990.4808327193770695No Hit
TGAGGTAGTAGATTGTATAGTT399020.4679671984337137No Hit
TTCAAGTAATTCAGGATAGGTT374980.4397732947438072No Hit
CCCATAAAGTAGAAAGCACT374510.43922208281642555No Hit
CAACACCAGTCGATGGGCTGT370200.43416735216320185No Hit
TGTAAACATCCCCGACTGGAAGCT363020.4257467103789453No Hit
CATTGCACTTGTCTCGGTCTGA357320.41906179977027364No Hit
ACCGGGTGCTGTAGGCTTT355030.4163761076134564No Hit
CAACGGAATCCCAAAAGCAGCTGT344920.40451918721807567No Hit
TCGTACGACTCTTAGCGG317470.37232606507631466No Hit
CCCATAAAGTAGAAAGCACTAC302240.354464453046478No Hit
CACCCGGCTGTGTGCACATGTGC280230.3286513157663265No Hit
ACTGGACTTGGAGTCAGAAGGC259040.3037998673807558No Hit
GCCTGTCTGAGCGTCGCT254760.29878032046757785No Hit
TGATGTAGTAGGTTGTATAGTT238650.27988665206306895No Hit
TATTGCACTTGTCCCGGCCTGTT237420.27844411872119773No Hit
CACTAGATTGTGAGCTCCTGGA221520.25979673649700835No Hit
TTCTCACTACTGCACTTGACTAG215590.25284208388132007No Hit
CAACGGAATCCCAAAAGCAGCT214970.2521149532537102No Hit
CACTAGATTGTGAGCTCCTGGAA197490.2316145607204504No Hit
TATTGCACTTGTCCCGGCCTGTAG184930.21688430155467564No Hit
TGTAAACATCCTACACTCTCAGCT180270.21141909393425284No Hit
CCCCCACTGCTAAATTTGACTGGCTT175510.20583660718034458No Hit
CAAAGAATTCTCCTTTTGGGCT171050.20060595782689272No Hit
AACATTCAACGCTGTCGGTGAGTT163140.1913291783681922No Hit
TAGCTTATCAGACTGATGTTGACA162030.19002737998650351No Hit
AGCTCGGTCTGAGGCCCCTCAGT161940.1899218287663666No Hit
CGCCTGTCTGAGCGTCGCT161750.1896989984127442No Hit
TTCACAGTGGCTAAGTTCCG157330.18451526071268654No Hit
TGAGGTAGTAGTTTGTACAGTT153030.1794722579728114No Hit
ACCCCCCACTGCTAAATTTGACTG149660.17551995117435115No Hit
AACGGAATCCCAAAAGCAGCTG148820.17453480645307323No Hit
TGATGTAGTAGATTGTATAGTT148030.17360830129853802No Hit
CTTCTCACTACTGCACTTGACTAG147950.17351447799174965No Hit
CTCCCACTGCTTCACTTGACTAG146620.1719546655163929No Hit
TGAGAACTGAATTCCATAGGCTGT144950.16999610398718562No Hit
TAGCAGCACGTAAATATTGGC135090.1584323814255185No Hit
ACCTGGTGCTGTAGGCTTA133190.1562040778892946No Hit
TATTGCACTTGTCCCGGCCTGTTT130990.1536239369526143No Hit
CCCCCCACTGCTAAATTTGACTGG121920.14298671954548237No Hit
TATTGCACTTGTCCCGGCCTGA121170.14210712604434136No Hit
CCCATAAAGTAGAAAGCACTA121110.1420367585642501No Hit
ATCCCACCGCTGCCACCA118030.13842456125289768No Hit
CCCCCCACTGCTAAATTTGACTGT116950.13715794661125463No Hit
ACCTGGTGCTGTAGGCTT116090.1361493460632796No Hit
TTCACAGTGGCTAAGTTCTG115960.1359968831897485No Hit
GACCCGCGGGCGCTCTCCAGTCCT114440.1342142403607694No Hit
TGAGGTAGTAGTTTGTGCTGTT110980.1301563823421722No Hit
ACTTGACTTGGAGTCAGAAGGC107170.12568804735637587No Hit
TGAGAACTGAATTCCATGGGTT105420.12363566252038018No Hit
TTCAAGTAATCCAGGATAGGC103230.12106724949704845No Hit
CGCCCCACTGCTAAATTTGACTG101690.11926115084137223No Hit
ACCTGGTGCTGTAGGCTTT101530.11907350422779549No Hit
AGCAGCATTGTACAGGGCTATGT101230.11872166682733909No Hit
TGTCAGTTTGTCAAATACCCCAA99660.11688038443161723No Hit
TGTAAACATCCCCGACTGGAAGC98730.1157896884902024No Hit
AGCAGCATTGTACAGGGCTATG94320.11061767870349326No Hit
AGCTACATTGTCTGCTGGGTTT93900.1101251063428543No Hit
TATTGCACTTGTCCCGGCCTG93850.11006646677611157No Hit
TTCAAGTAATCCAGGATAGGCTT93440.10958562232882113No Hit
CCCCCCACTGCTAAATTTGACTGGCT93170.10926896866841036No Hit
TTATAAAGCAATGAGACTGATT92740.10876466839442285No Hit
TATTGCACTTGTCCCGGCCTGTAAT92630.10863566134758885No Hit
CAAAGAATTCTCCTTTTGGGCTT92040.10794371446002457No Hit
ACCACAGGGTAGAACCACGGAC90860.10655982068489608No Hit
ACTGGACTTGGAGTCAGAAGGCA90250.10584441797063472No Hit
TGTCAGTTTGTCAAATACCCCA89640.10512901525637336No Hit
ACTGGACTTGGAGTCAGAAGGCT87500.10261924179978436No Hit
CTCCCCACTGCTAAATTTGACTG87140.10219703691923666No Hit
TGCAAGTAATCCAGGATAGGCT86600.10156372959841514No Hit
AGCTACATCTGGCTACTGGGTCTC85650.10044957783030319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)