FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6055756
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG75381012.447826497632995No Hit
CAACGGAATCCCAAAAGCAGCTG6045979.98384016793279No Hit
CCCCCACTGCTAAATTTGACTG2267983.745164105026689No Hit
TTCAAGTAATCCAGGATAGGCT2076663.4292332782232307No Hit
TAGCAGCACGTAAATATTGGCG2050383.385836549557149No Hit
AACATTCAACGCTGTCGGTGAGT1739402.8723085936751747No Hit
AAGCTGCCAGTTGAAGAACTGT1262182.0842649538719855No Hit
AACATTCAACGCTGTCGGTGAG1240712.048811081556126No Hit
TAGCTTATCAGACTGATGTTGACT1115251.8416362878557195No Hit
TAGCTTATCAGACTGATGTTGAC1052561.7381149438649774No Hit
TATTGCACTTGTCCCGGCCTGTAA1020651.6854212752297153No Hit
TATTGCACTTGTCCCGGCCTGTAT879451.4522546813312822No Hit
TAGCTTATCAGACTGATGTTGA850081.4037553692718134No Hit
TGAGGTAGTAGGTTGTATAGTT689331.1383054403116637No Hit
TGTAAACATCCTTGACTGGAAGCT578860.955883955694384No Hit
TGAGGTAGTAGATTGTATAGTT450640.7441515146911467No Hit
TATTGCACTTGTCCCGGCCTGT399700.6600331981671653No Hit
CCCCCACTGCTAAATTTGACTGGCTT384540.6349991644313279No Hit
TTCAAGTAATTCAGGATAGGTT349690.5774506106256593No Hit
TTCTCACTACTGCACTTGACTAG347470.5737846769255565No Hit
TATTGCACTTGTCCCGGCCTGTA343050.5664858359550815No Hit
TGTAAACATCCCCGACTGGAAGCT339250.5602108143062567No Hit
CAACGGAATCCCAAAAGCAGCTGT329160.5435489805071406No Hit
AGCAGCATTGTACAGGGCTATGA328430.5423435158219717No Hit
CATAAAGTAGAAAGCACTACT326160.5385950160475422No Hit
ACTGGACTTGGAGTCAGAAGGC271740.4487301007504265No Hit
CTTCTCACTACTGCACTTGACTAG269770.44547699742195684No Hit
CAAAGAATTCTCCTTTTGGGCT209760.34638119501512277No Hit
CAACGGAATCCCAAAAGCAGCT201390.3325596341728432No Hit
CACCCGGCTGTGTGCACATGTGC198720.32815060580380057No Hit
TAGCTTATCAGACTGATGTTGACA193500.31953070764409924No Hit
CCCCCCACTGCTAAATTTGACTGGCT187110.3089787633451546No Hit
GGAATACCGGGTGCTGTAGGCTTT183720.3033807835058084No Hit
TGAGGTAGTAGTTTGTACAGTT170360.28131912844573No Hit
ACCCCCCACTGCTAAATTTGACTG169440.27979991267811977No Hit
CCCATAAAGTAGAAAGCACTAC165900.2739542346157936No Hit
CATTGCACTTGTCTCGGTCTGA165570.2734092985252378No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC158460.2616684027559895No Hit
AACGGAATCCCAAAAGCAGCTG156100.25777128404777205No Hit
GACCCGCGGGCGCTCTCCAGTCCT145020.23947464197698853No Hit
CCCCCCACTGCTAAATTTGACTGG144850.23919391732427792No Hit
TGTAAACATCCTACACTCTCAGCT141510.23367850355925832No Hit
TGTCAGTTTGTCAAATACCCCAA139470.2303098077267314No Hit
TAGCAGCACGTAAATATTGGC135320.22345682355762023No Hit
CCCATAAAGTAGAAAGCACT133680.2207486563197064No Hit
CAAAGAATTCTCCTTTTGGGCTT132800.21929549341155752No Hit
CTCCCACTGCTTCACTTGACTAG129060.21311955105192482No Hit
GGAATACCGGGTGCTGTAGGCTT127330.21026276488022305No Hit
AACATTCAACGCTGTCGGTGAGTT116080.19168539815672891No Hit
TGTAAACATCCCCGACTGGAAGC114790.18955519343910157No Hit
TGAGGTAGTAGTTTGTGCTGTT112790.18625255046603595No Hit
TATTGCACTTGTCCCGGCCTGTAG110460.18240497140241452No Hit
CACTAGATTGTGAGCTCCTGGAA99700.16463675220732144No Hit
CAACACCAGTCGATGGGCTGT98570.16277075892753937No Hit
CTCCCACTGCTTCACTTGACTAGCCT97510.16102035815181456No Hit
TGTCAGTTTGTCAAATACCCCA95060.15697462050980918No Hit
CACTAGATTGTGAGCTCCTGGA93180.15387013611512748No Hit
GGGAATACCGGGTGCTGTAGGCTTT92200.15225184105832534No Hit
TTCAAGTAATCCAGGATAGGCTT91850.15167387853803885No Hit
TATTGCACTTGTCCCGGCCTGTAAA90430.14932900202716226No Hit
TATTGCACTTGTCCCGGCCTGTAAT90290.14909781701904767No Hit
ACTGGACTTGGAGTCAGAAGGCT89080.14709971802034297No Hit
CTACGGGGATGATTTTACGAACTGAA86980.14363194289862405No Hit
TATTGCACTTGTCCCGGCCTGTT84980.14032929992555843No Hit
GTCAGGATGGCCGAGCGGTCTAAGG83030.13710922302681944No Hit
TTCAAGTAATCCAGGATAGGC78780.13009110670905497No Hit
ACTGGACTTGGAGTCAGAAGGCA77700.12830767950359956No Hit
GGGAATACCGGGTGCTGTAGGCTT77360.1277462301981784No Hit
CAACGGAATCCCAAAAGCAGCTGA76050.1255829990508204No Hit
ACCGGGTGCTGTAGGCTTA75780.12513714224945655No Hit
ACCGGGTGCTGTAGGCTT74070.12231338250748543No Hit
AGCTCGGTCTGAGGCCCCTCAGT72020.11892817346009317No Hit
CCCCCACTGCTAAATTTGACTGGCT70260.11602184764379542No Hit
ATGCACCTGGGCAAGGATTCTGA67860.11205867607611668No Hit
ACCGGGTGCTGTAGGCTTT67660.11172841177881011No Hit
TATTGCACTTGTCCCGGCCTGTTT67640.11169538534907945No Hit
TTCACAGTGGCTAAGTTCTGC65810.10867346702872441No Hit
TGAGAACTGAATTCCATAGGCTGT63560.10495799368402557No Hit
CCCCCCACTGCTAAATTTGACTGT63250.10444608402320041No Hit
TTCACAGTGGCTAAGTTCCG63080.10416535937048982No Hit
ATCACATTGCCAGGGATTTCC63000.10403325365156721No Hit
TCGTTTCCCGGCCAATGCACCA62840.10376904221372195No Hit
AACACCCTGATTGCTCCTATCTGATT62510.10322410612316611No Hit
CCCCCACTGCTAAATTTGACTGGCTA61810.10206818108259316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)