FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9230635
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5478135.934727134157076No Hit
CCCCCCACTGCTAAATTTGACTG4916715.3265132897140886No Hit
TAGCAGCACGTAAATATTGGCG4042464.379395350374053No Hit
AAGCTGCCAGTTGAAGAACTGT4030954.366926002382285No Hit
TTCAAGTAATCCAGGATAGGCT3061833.3170307351552735No Hit
AACATTCAACGCTGTCGGTGAGT3052323.3067280853375745No Hit
TAGCTTATCAGACTGATGTTGAC2404152.604533707594331No Hit
TAGCTTATCAGACTGATGTTGA2129262.306731877059379No Hit
TAGCTTATCAGACTGATGTTGACT1776301.924352983299632No Hit
CCCCCACTGCTAAATTTGACTG1645511.7826617562063714No Hit
AACATTCAACGCTGTCGGTGAG1433861.5533709219354899No Hit
CATAAAGTAGAAAGCACTACT1310861.420118984230229No Hit
GCCTGTCTGAGCGTCGCT1082341.172552050861073No Hit
TGTAAACATCCTTGACTGGAAGCT848710.9194492036571699No Hit
TATTGCACTTGTCCCGGCCTGTAA806240.8734393679308087No Hit
TATTGCACTTGTCCCGGCCTGT803550.8705251588866856No Hit
TGAGGTAGTAGGTTGTATAGTT762130.8256528397017106No Hit
TATTGCACTTGTCCCGGCCTGTAT741200.8029783433100757No Hit
TCCTGTCTGAGCGTCGCT651060.7053252566047732No Hit
AGCAGCATTGTACAGGGCTATGA599660.6496411135311926No Hit
TTCAAGTAATTCAGGATAGGTT503980.5459862728837181No Hit
TATTGCACTTGTCCCGGCCTGTA423710.4590258416674476No Hit
CGCCTGTCTGAGCGTCGCT423560.4588633393043924No Hit
CCCATAAAGTAGAAAGCACTAC423010.45826749730652333No Hit
CATTGCACTTGTCTCGGTCTGA403590.43722885803631056No Hit
ACTGGACTTGGAGTCAGAAGGC403470.43709885614586647No Hit
TGAGGTAGTAGATTGTATAGTT400620.4340113112478177No Hit
TGTAAACATCCCCGACTGGAAGCT395090.4280203907965162No Hit
TGTAAACATCCTACACTCTCAGCT392060.4247378430628012No Hit
CCCATAAAGTAGAAAGCACT376680.4080759341042084No Hit
CAACGGAATCCCAAAAGCAGCTGT339190.36746117683127977No Hit
CTTCTCACTACTGCACTTGACTAG317170.34360582993477695No Hit
CAACACCAGTCGATGGGCTGT308680.3344081961858529No Hit
AACCGGGCGGAAACACCA294390.31892713773212783No Hit
TGATGTAGTAGGTTGTATAGTT287500.31146286252245914No Hit
TGTCAGTTTGTCAAATACCCCAA287320.31126785968679294No Hit
TTCTCACTACTGCACTTGACTAG268980.2913992374305776No Hit
GGAATACCGGGTGCTGTAGGCTTT235090.25468453687097364No Hit
TCGTACGACTCTTAGCGG232770.2521711669890533No Hit
AACATTCAACGCTGTCGGTGAGTT230960.2502103051415206No Hit
TAGCTTATCAGACTGATGTTGACA228180.24719859467956432No Hit
TGTAAACATCCTACACTCAGCT219590.23789262602193673No Hit
CTCCCACTGCTTCACTTGACTAG211780.2294316696521962No Hit
TGAGGTAGTAGTTTGTACAGTT207050.22430742847052232No Hit
TTCAAGTAATCCAGGATAGGCTT187800.2034529585451055No Hit
TGTCAGTTTGTCAAATACCCCA176360.19105944498942923No Hit
ACTTGACTTGGAGTCAGAAGGC175120.18971609212150628No Hit
TTATAAAGCAATGAGACTGATT172510.18688855100434584No Hit
CCCCCCACTGCTAAATTTGACTGGCT169620.18375767214281574No Hit
TAGCAGCACGTAAATATTGGCGT169170.18327016505365015No Hit
CAAAGAATTCTCCTTTTGGGCTT167560.18152597302352438No Hit
GGAATACCGGGTGCTGTAGGCTT166200.18005261826515725No Hit
TAGCAGCACGTAAATATTGGC164400.17810258990849492No Hit
TGAATACCGGGTGCTGTAGGCTTT162640.17619589551531395No Hit
TGCAAGTAATCCAGGATAGGCT162000.1755025520996118No Hit
CAAAGAATTCTCCTTTTGGGCT157030.17011830713704962No Hit
CAACGGAATCCCAAAAGCAGCT156610.16966330052049508No Hit
CTACGCCTGTCTGAGCGTCGCT156230.16925162786742193No Hit
TGATGTAGTAGATTGTATAGTT152280.16497239897363508No Hit
TTCACAGTGGCTAAGTTCCG148140.16048733375331167No Hit
CACTAGATTGTGAGCTCCTGGA147600.15990232524631295No Hit
TTCAAGTAATCCAGGATAGGC145370.15748645678222573No Hit
CCCCCCACTGCTAAATTTGACTGG142810.15471308311941703No Hit
AGCAGCATTGTACAGGGCTATGT139830.1514847028400538No Hit
CCCCCACTGCTAAATTTGACTGGCTT135310.14658796496665724No Hit
CACCCGGCTGTGTGCACATGTGC133090.14418292999344032No Hit
TGTAAACATCCCCGACTGGAAGC130910.1418212289837048No Hit
TGAGGTAGTAGTTTGTGCTGTT129650.14045620913404117No Hit
TACGCCTGTCTGAGCGTCGCT127730.13837617888693465No Hit
TATTGCACTTGTCCCGGCCTGTT126260.1367836557289937No Hit
ACCGGGTGCTGTAGGCTT126060.1365669859115868No Hit
AACATTCAACGCTGTCGGTGA124340.13470362548188722No Hit
ACCACAGGGTAGAACCACGGAC123050.13330610515961253No Hit
TTCACAGTGGCTAAGTTCCGC122210.13239609192650342No Hit
ACGCCTGTCTGAGCGTCGCT120760.1308252357503032No Hit
TAGCAGCACATAATGGTTTGT118840.1287452055031967No Hit
AGCAGCATTGTACAGGGCTATG116570.12628600307562804No Hit
TAGCTTATCAGACTGATGTTG116310.12600433231299907No Hit
CAAAGTGCTGTTCGTGCAGGT115720.12536515635164863No Hit
TGAATACCGGGTGCTGTAGGCTT113150.1225809491979696No Hit
TGTCAGTTTGTCAAATACCCC112720.1221151090905447No Hit
CCCATAAAGTAGAAAGCACTA111480.12077175622262172No Hit
AACGGAATCCCAAAAGCAGCTG108670.1177275452880544No Hit
TTTAAACATCCTTGACTGGAAGCT107800.11678503158233425No Hit
CATAAAGTAGAAAGCACTAC107010.11592918580357689No Hit
CACTAGATTGTGAGCTCCTGGAA102750.11131411869280933No Hit
GCGTTCGCGCTTTCCCCTG102350.11088077905799547No Hit
CAAAGTGCTGTTCGTGCAGGTAG101930.11042577244144093No Hit
ACTGGACTTGGAGTCAGAAGG100050.10838907615781579No Hit
ACCGGGTGCTGTAGGCTTT97330.10544236664108157No Hit
TTCACAGTGGCTAAGTTCTGC96420.10445651897188005No Hit
CAGTGCAATAGTATTGTCAAAGC93950.10178064672690448No Hit
CTCCCACTGCTTCACTTGACTAGCCT93420.10120647171077612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)