FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8129558
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG90299611.107565749577038No Hit
CCCCCCACTGCTAAATTTGACTG85442810.510140895728895No Hit
TAGCAGCACGTAAATATTGGCG2803143.4480841393837154No Hit
AACATTCAACGCTGTCGGTGAGT2670633.2850863478678667No Hit
CCCCCACTGCTAAATTTGACTG2298342.827140171704292No Hit
TTCAAGTAATCCAGGATAGGCT2241952.757776007010467No Hit
AACATTCAACGCTGTCGGTGAG2014412.4778837914681215No Hit
AAGCTGCCAGTTGAAGAACTGT1957482.4078553840196477No Hit
TAGCTTATCAGACTGATGTTGACT1751012.153880936700372No Hit
TAGCTTATCAGACTGATGTTGAC1641962.0197408026365027No Hit
TATTGCACTTGTCCCGGCCTGTAA1488661.8311696650666616No Hit
TATTGCACTTGTCCCGGCCTGTAT1268161.5599372069182604No Hit
TAGCTTATCAGACTGATGTTGA1091571.3427175253562371No Hit
TGTAAACATCCTTGACTGGAAGCT802100.9866465064890367No Hit
TGAGGTAGTAGGTTGTATAGTT630130.7751097907167893No Hit
TGTAAACATCCCCGACTGGAAGCT555860.6837518103690262No Hit
TATTGCACTTGTCCCGGCCTGT496110.6102545796462735No Hit
CAACGGAATCCCAAAAGCAGCTGT469420.5774237664581519No Hit
TTCAAGTAATTCAGGATAGGTT459940.5657626158765335No Hit
TATTGCACTTGTCCCGGCCTGTA440750.5421573965029833No Hit
AGCAGCATTGTACAGGGCTATGA438930.5399186524039806No Hit
TGAGGTAGTAGATTGTATAGTT405800.4991661293270802No Hit
CCCCCACTGCTAAATTTGACTGGCTT387450.476594176460762No Hit
CATAAAGTAGAAAGCACTACT370300.4554983186047753No Hit
CTTCTCACTACTGCACTTGACTAG324150.3987301646657788No Hit
CAAAGAATTCTCCTTTTGGGCT311290.38291134647172703No Hit
ACTGGACTTGGAGTCAGAAGGC308560.379553230323223No Hit
TAGCTTATCAGACTGATGTTGACA290220.3569935782486576No Hit
CCCATAAAGTAGAAAGCACTAC286250.3521101639228111No Hit
CAACGGAATCCCAAAAGCAGCT281950.3468208234691234No Hit
TTCTCACTACTGCACTTGACTAG281410.34615658071447425No Hit
GGAATACCGGGTGCTGTAGGCTTT271620.33411410558852034No Hit
CACCCGGCTGTGTGCACATGTGC267790.3294029023472125No Hit
TTAATGCTAATCGTGATAGGGGTT255290.31402691265626004No Hit
TGTCAGTTTGTCAAATACCCCAA242440.29822039525396093No Hit
TGAGAACTGAATTCCATAGGCTGT235610.2898189544868245No Hit
CCCCCCACTGCTAAATTTGACTGGCT233600.28734649534451934No Hit
TGTAAACATCCTACACTCTCAGCT228500.2810730915506108No Hit
ACCCCCCACTGCTAAATTTGACTG218110.2682925689194911No Hit
CAAAGAATTCTCCTTTTGGGCTT217770.2678743419998972No Hit
TAGCAGCACGTAAATATTGGC206120.2535439196079295No Hit
CATTGCACTTGTCTCGGTCTGA204730.2518341095542956No Hit
TGAGAACTGAATTCCATGGGTT177790.21869577657235487No Hit
CCCATAAAGTAGAAAGCACT177120.2178716235249198No Hit
TGATGTAGTAGGTTGTATAGTT172240.21186883714957197No Hit
TGTAAACATCCCCGACTGGAAGC170540.20977770255160244No Hit
CTCCCACTGCTTCACTTGACTAG170240.2094086787990196No Hit
TATTGCACTTGTCCCGGCCTGTAG170030.20915036217221158No Hit
CACTAGATTGTGAGCTCCTGGAA169170.20809249408147404No Hit
TGAGGTAGTAGTTTGTACAGTT168750.20757586082785803No Hit
CCCCCCACTGCTAAATTTGACTGG168630.20742825132682488No Hit
TGTCAGTTTGTCAAATACCCCA167910.20654259432062605No Hit
CTCCCACTGCTTCACTTGACTAGCCT164970.20292616154531404No Hit
AACATTCAACGCTGTCGGTGAGTT164060.20180678949581268No Hit
GGAATACCGGGTGCTGTAGGCTT159360.19602541737201457No Hit
AACGGAATCCCAAAAGCAGCTG154540.1900964357471833No Hit
TATTGCACTTGTCCCGGCCTGTAAT144210.1773897178665802No Hit
CACTAGATTGTGAGCTCCTGGA143400.1763933537346065No Hit
TATTGCACTTGTCCCGGCCTGTAAA137880.1696033166870819No Hit
TTCAAGTAATCCAGGATAGGCTT123090.15141044568474693No Hit
GGGAATACCGGGTGCTGTAGGCTTT123070.15138584410124142No Hit
CAACACCAGTCGATGGGCTGT119870.1474495907403576No Hit
TGAATACCGGGTGCTGTAGGCTTT113620.13976159589488135No Hit
TGATGTAGTAGATTGTATAGTT112700.13862992305362726No Hit
ATGCACCTGGGCAAGGATTCTGA108700.1337096063525225No Hit
TATTGCACTTGTCCCGGCCTGTT103830.1277191207689274No Hit
ACTGGACTTGGAGTCAGAAGGCA102830.1264890415936512No Hit
TATTGCACTTGTCCCGGCCTGTTT101640.12502524737507253No Hit
TCGTTTCCCGGCCAATGCACCA101610.12498834499981425No Hit
ACTGGACTTGGAGTCAGAAGGCT97630.12009262988221499No Hit
ACTTGACTTGGAGTCAGAAGGC97100.11944068791931862No Hit
ATCACATTGCCAGGGATTTCCA96920.1192192736677689No Hit
TGAGGTAGTAGTTTGTGCTGTT95950.118026096867751No Hit
ATCACATTGCCAGGGATTTCC95630.1176324715316626No Hit
CAACGGAATCCCAAAAGCAGCTGA94090.11573814960173727No Hit
AGCTCGGTCTGAGGCCCCTCAGT90850.11175269307384238No Hit
GGGAATACCGGGTGCTGTAGGCTT89020.10950164818308696No Hit
TTCAAGTAATCCAGGATAGGC86270.10611893045107741No Hit
AGCAGCATTGTACAGGGCTATGT84490.10392938951908579No Hit
TAGCAGCACATCATGGTTTACA83150.10228108342421567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)