FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7667770
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6056837.899076263372532No Hit
CAACGGAATCCCAAAAGCAGCTG5210776.795678534958665No Hit
TAGCAGCACGTAAATATTGGCG2478763.2326999896971347No Hit
TTCAAGTAATCCAGGATAGGCT2452453.1983875364023704No Hit
TAGCTTATCAGACTGATGTTGACT2074872.705962750578069No Hit
TAGCTTATCAGACTGATGTTGAC2074722.7057671265570042No Hit
CCCCCACTGCTAAATTTGACTG1994262.6008344016578486No Hit
TAGCTTATCAGACTGATGTTGA1750842.2833757402739No Hit
AAGCTGCCAGTTGAAGAACTGT1693812.2089994874650647No Hit
AACATTCAACGCTGTCGGTGAGT1530541.996069261336738No Hit
GCCTGTCTGAGCGTCGCT1501481.9581703676557851No Hit
AACATTCAACGCTGTCGGTGAG1490271.9435507324815429No Hit
TATTGCACTTGTCCCGGCCTGTAT1304181.7008595719485586No Hit
TATTGCACTTGTCCCGGCCTGTAA1214561.5839807401630464No Hit
TGTAAACATCCTTGACTGGAAGCT904201.1792215989785817No Hit
TGAGGTAGTAGGTTGTATAGTT864001.126794361333217No Hit
CATAAAGTAGAAAGCACTACT754010.9833497874871051No Hit
TGAGGTAGTAGATTGTATAGTT704960.9193807325989173No Hit
TTCAAGTAATTCAGGATAGGTT698410.9108384836790878No Hit
TATTGCACTTGTCCCGGCCTGT614100.800884742239269No Hit
TATTGCACTTGTCCCGGCCTGTA601760.7847914061063386No Hit
TCCTGTCTGAGCGTCGCT478660.6242492928191639No Hit
AGCAGCATTGTACAGGGCTATGA439210.5728001752791229No Hit
TGTAAACATCCCCGACTGGAAGCT435800.5683529892002498No Hit
TTAATGCTAATCGTGATAGGGGTT389220.507605209858929No Hit
TAGCTTATCAGACTGATGTTGACA299370.39042642124111704No Hit
CAAAGAATTCTCCTTTTGGGCT293320.38253625239150363No Hit
TGAGAACTGAATTCCATAGGCTGT283150.3692729437633106No Hit
TGAGGTAGTAGTTTGTACAGTT276450.3605350708224164No Hit
CATTGCACTTGTCTCGGTCTGA276110.36009165637466956No Hit
GGAATACCGGGTGCTGTAGGCTTT261170.34060750387661604No Hit
CAACGGAATCCCAAAAGCAGCTGT254260.33159575730623114No Hit
TTCTCACTACTGCACTTGACTAG253720.3308915108303979No Hit
TGTAAACATCCTACACTCTCAGCT250140.32622261752765147No Hit
CCCATAAAGTAGAAAGCACTAC238100.3105205294368506No Hit
TGATGTAGTAGGTTGTATAGTT228490.29798755048729947No Hit
ACTGGACTTGGAGTCAGAAGGC223470.291440666582331No Hit
CCCATAAAGTAGAAAGCACT214270.2794423932903569No Hit
TGAGAACTGAATTCCATGGGTT205900.26852657291494136No Hit
CCCCCACTGCTAAATTTGACTGGCTT200620.2616406073734606No Hit
CACTAGATTGTGAGCTCCTGGAA197060.2569977972735228No Hit
CTTCTCACTACTGCACTTGACTAG190600.24857292276633233No Hit
TGATGTAGTAGATTGTATAGTT189340.24692968098938806No Hit
CAACACCAGTCGATGGGCTGT181100.23618340143222866No Hit
TATTGCACTTGTCCCGGCCTGTAG175930.2294408935061954No Hit
CACCCGGCTGTGTGCACATGTGC174480.22754986130256905No Hit
CAACGGAATCCCAAAAGCAGCT172390.22482416660906623No Hit
TGTCAGTTTGTCAAATACCCCA166810.21754695302545593No Hit
TAGCAGCACGTAAATATTGGC165060.21526467277969996No Hit
CACTAGATTGTGAGCTCCTGGA161570.2107131538895924No Hit
ACCGGGTGCTGTAGGCTTA159240.20767446076238594No Hit
CTCCCACTGCTTCACTTGACTAG156060.2035272315158123No Hit
GGAATACCGGGTGCTGTAGGCTT151770.19793238451335915No Hit
CAAAGAATTCTCCTTTTGGGCTT147760.192702702350227No Hit
TATTGCACTTGTCCCGGCCTGTT145190.18935101078931685No Hit
ACCCCCCACTGCTAAATTTGACTG141560.1846169094795488No Hit
AACCGGGCGGAAACACCA134360.17522695646843867No Hit
TTATAAAGCAATGAGACTGATT129540.1689409045915566No Hit
TTCACAGTGGCTAAGTTCCG124980.16299393435118684No Hit
TATTGCACTTGTCCCGGCCTGTTT124550.16243314549080112No Hit
TGTAAACATCCCCGACTGGAAGC123360.16088119492368708No Hit
TTCAAGTAATCCAGGATAGGCTT123210.16068557090262228No Hit
AGCTCGGTCTGAGGCCCCTCAGT119380.15569063756476786No Hit
ACCGGGTGCTGTAGGCTT119360.15566455436195922No Hit
TGAGGTAGTAGTTTGTGCTGTT113460.14797000953341063No Hit
ACCGGGTGCTGTAGGCTTT112630.1468875566168521No Hit
TGTCAGTTTGTCAAATACCCCAA111260.1451008572244603No Hit
CCCCCCACTGCTAAATTTGACTGGCT110940.14468352597952208No Hit
AACGGAATCCCAAAAGCAGCTG110570.14420098672756226No Hit
TTCAAGTAATCCAGGATAGGC108680.14173612406214584No Hit
TATTGCACTTGTCCCGGCCTGTAAA107710.14047108872592684No Hit
AACATTCAACGCTGTCGGTGAGTT105520.13761497801838082No Hit
TGAATACCGGGTGCTGTAGGCTTT102880.13417199524764045No Hit
TATTGCACTTGTCCCGGCCTGTAAT102090.1331417087366992No Hit
TGTCAGTTTGTCAAATACC101390.13222879663839682No Hit
TGTCAGTTTGTCAAATACCCC100010.13042905564460072No Hit
CCCCCCACTGCTAAATTTGACTGG92970.12124776825595969No Hit
AGCAGCATTGTACAGGGCTATGT91900.11985231690569748No Hit
CTCCCACTGCTTCACTTGACTAGCCT88630.11558771324648497No Hit
TCAGTGCACTACAGAACTTTGT87950.11470088435099123No Hit
TCGTACGACTCTTAGCGG87450.11404880428077524No Hit
TTCACAGTGGCTAAGTTCTG86880.11330543300072903No Hit
ATCACATTGCCAGGGATTTCC84740.11051453030020462No Hit
ATCCCGGACGAGCCCCCA82130.1071106723336772No Hit
TGTCAGTTTGTCAAATACCC81840.10673246589295193No Hit
CCCATAAAGTAGAAAGCACTA81260.10597605301150138No Hit
ACTGGACTTGGAGTCAGAAGGCA79560.10375898077276706No Hit
TGTAAACATCCTACACTCAGCT78840.10281998547165604No Hit
AGCTACATTGTCTGCTGGGTTT77640.10125499330313767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)