FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8153288
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7981759.78960880567447No Hit
CCCCCCACTGCTAAATTTGACTG6546898.029754376393916No Hit
TAGCAGCACGTAAATATTGGCG3060823.754092827335426No Hit
TTCAAGTAATCCAGGATAGGCT2906913.5653223582927525No Hit
AACATTCAACGCTGTCGGTGAGT2274682.7898928628548383No Hit
AAGCTGCCAGTTGAAGAACTGT2186622.681887356364696No Hit
CCCCCACTGCTAAATTTGACTG2114762.593751134511623No Hit
TAGCTTATCAGACTGATGTTGAC1895052.324277027869002No Hit
AACATTCAACGCTGTCGGTGAG1816522.2279600573412837No Hit
TAGCTTATCAGACTGATGTTGACT1746612.142215508638969No Hit
TAGCTTATCAGACTGATGTTGA1645592.018314574439171No Hit
TATTGCACTTGTCCCGGCCTGTAA1451991.7808643580356782No Hit
TATTGCACTTGTCCCGGCCTGTAT1201161.4732216009050583No Hit
TGTAAACATCCTTGACTGGAAGCT895611.0984648156670045No Hit
TGAGGTAGTAGGTTGTATAGTT830181.0182149827161753No Hit
TATTGCACTTGTCCCGGCCTGT645080.7911900082518856No Hit
CATAAAGTAGAAAGCACTACT606170.7434669301513697No Hit
TTCAAGTAATTCAGGATAGGTT603280.7399223478920406No Hit
AGCAGCATTGTACAGGGCTATGA562130.6894519119157817No Hit
TGAGGTAGTAGATTGTATAGTT544790.6681844183598077No Hit
TATTGCACTTGTCCCGGCCTGTA542700.6656210353418155No Hit
TGTAAACATCCCCGACTGGAAGCT530970.6512342014657154No Hit
CCCCCACTGCTAAATTTGACTGGCTT411190.504324145056571No Hit
CTTCTCACTACTGCACTTGACTAG387460.47521932256041977No Hit
CAACGGAATCCCAAAAGCAGCTGT376320.4615561231248056No Hit
TTCTCACTACTGCACTTGACTAG375590.4606607788170858No Hit
ACTGGACTTGGAGTCAGAAGGC351440.43104082671923283No Hit
CATTGCACTTGTCTCGGTCTGA333080.40852230413055446No Hit
CAAAGAATTCTCCTTTTGGGCT301920.3703045936804882No Hit
TAGCTTATCAGACTGATGTTGACA297970.365459922426388No Hit
CAACGGAATCCCAAAAGCAGCT278350.34139601103260425No Hit
CTCCCACTGCTTCACTTGACTAG275070.3373730941431236No Hit
CCCATAAAGTAGAAAGCACT272450.33415966662774577No Hit
CACCCGGCTGTGTGCACATGTGC265750.3259421229815505No Hit
CCCATAAAGTAGAAAGCACTAC245990.30170650172053287No Hit
TGTAAACATCCTACACTCTCAGCT245020.30051679764041206No Hit
CCCCCCACTGCTAAATTTGACTGGCT243660.2988487589301396No Hit
TGATGTAGTAGGTTGTATAGTT232120.284694959873857No Hit
TGAGGTAGTAGTTTGTACAGTT219680.2694373116710706No Hit
TAGCAGCACGTAAATATTGGC201790.24749524363667763No Hit
CAAAGAATTCTCCTTTTGGGCTT200750.24621968462293983No Hit
CAACACCAGTCGATGGGCTGT198110.24298172712652857No Hit
TGTAAACATCCCCGACTGGAAGC181260.22231521810587335No Hit
ACCCCCCACTGCTAAATTTGACTG171420.2102464674374314No Hit
CACTAGATTGTGAGCTCCTGGAA164740.20205345377226955No Hit
AACGGAATCCCAAAAGCAGCTG163720.2008024247395652No Hit
TATTGCACTTGTCCCGGCCTGTAG159960.1961907883052825No Hit
CTCCCACTGCTTCACTTGACTAGCCT158330.19419159485105886No Hit
CACTAGATTGTGAGCTCCTGGA158070.19387270509762441No Hit
CCCCCCACTGCTAAATTTGACTGG156170.19154235689944962No Hit
TGATGTAGTAGATTGTATAGTT154910.18999696809434427No Hit
TTCAAGTAATCCAGGATAGGCTT151860.18625614598674792No Hit
AACATTCAACGCTGTCGGTGAGTT146000.17906886154395624No Hit
TGAGGTAGTAGTTTGTGCTGTT138880.1703361882960592No Hit
AGCTCGGTCTGAGGCCCCTCAGT135780.1665340412358793No Hit
TATTGCACTTGTCCCGGCCTGTT132530.1625479193179488No Hit
TGTCAGTTTGTCAAATACCCCA127870.1568324337371622No Hit
GGAATACCGGGTGCTGTAGGCTTT126680.15537289986567382No Hit
AGAATTACTCTGAGACCT123990.15207361741667902No Hit
TTCAAGTAATCCAGGATAGGC123870.15192643753047852No Hit
TATTGCACTTGTCCCGGCCTGTAAA116290.14262957471881282No Hit
TGTCAGTTTGTCAAATACCCCAA115900.14215124008866117No Hit
TTCACAGTGGCTAAGTTCCG113800.13957559208015222No Hit
AGCAGCATTGTACAGGGCTATGT110190.13514793050362015No Hit
CTACGGGGATGATTTTACGAACTGAA110020.13493942566483608No Hit
CAACGGAATCCCAAAAGCAGCTGA109470.13426485118641707No Hit
TATTGCACTTGTCCCGGCCTGTAAT107710.13210621285547622No Hit
TCGTTTCCCGGCCAATGCACCA104700.1284144507099467No Hit
TCGTACGACTCTTAGCGG104620.12831633078581303No Hit
ACTGGACTTGGAGTCAGAAGGCA101740.12478401351700075No Hit
ACTGGACTTGGAGTCAGAAGGCT101710.12474721854545062No Hit
TATTGCACTTGTCCCGGCCTGTTT100240.12294426493949435No Hit
AGCTCTAGAATTACTCTGAGACCT100230.12293199994897763No Hit
CCCATAAAGTAGAAAGCACTA98690.12104319140940441No Hit
ATGCACCTGGGCAAGGATTCTGA98250.1205035318266692No Hit
ACTTGACTTGGAGTCAGAAGGC98240.12049126683615248No Hit
TTCACAGTGGCTAAGTTCTGC97850.1200129322060008No Hit
ATCACATTGCCAGGGATTTCC96160.11794014880867694No Hit
TTATAAAGCAATGAGACTGATT95100.11664005981390575No Hit
GGGAATACCGGGTGCTGTAGGCTTT94860.11634570004150473No Hit
TTCACAGTGGCTAAGTTCTG90210.11064247945123488No Hit
GGAATACCGGGTGCTGTAGGCTT87300.10707336721087247No Hit
AGCAGCATTGTACAGGGCTATG84980.10422788941099591No Hit
ACTGGACTTGGAGTCAGAAGG83440.10233908087142268No Hit
TGTAAACATCCTACACTCAGCT82830.1015909164499034No Hit
ATCACATTGCCAGGGATTTCCA82680.10140694159215274No Hit
AACACCCTGATTGCTCCTATCTGATT81640.10013138257841499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)