FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7522013
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG87749811.665733627421277No Hit
CAACGGAATCCCAAAAGCAGCTG4566076.070276666631658No Hit
TAGCAGCACGTAAATATTGGCG2836213.7705465279041657No Hit
AACATTCAACGCTGTCGGTGAGT2627733.4933866772099433No Hit
AAGCTGCCAGTTGAAGAACTGT2462773.2740836794618673No Hit
CCCCCACTGCTAAATTTGACTG2420913.218433682579384No Hit
TTCAAGTAATCCAGGATAGGCT2406133.1987846870246037No Hit
TAGCTTATCAGACTGATGTTGAC1925802.5602189201215153No Hit
TAGCTTATCAGACTGATGTTGACT1626892.1628385912122194No Hit
AACATTCAACGCTGTCGGTGAG1213971.6138897925329294No Hit
TAGCTTATCAGACTGATGTTGA1053241.4002102894531026No Hit
TATTGCACTTGTCCCGGCCTGTAA806391.072040157335543No Hit
TATTGCACTTGTCCCGGCCTGTAT746290.9921413323800424No Hit
TGTAAACATCCTTGACTGGAAGCT707750.9409050476248845No Hit
CATAAAGTAGAAAGCACTACT534950.7111793079857746No Hit
TATTGCACTTGTCCCGGCCTGT479360.6372762184803457No Hit
AGCAGCATTGTACAGGGCTATGA409070.5438304879292285No Hit
TGAGGTAGTAGGTTGTATAGTT407400.5416103375519292No Hit
TGTAAACATCCCCGACTGGAAGCT382620.5086670283606263No Hit
TATTGCACTTGTCCCGGCCTGTA365260.4855880998876232No Hit
CCCATAAAGTAGAAAGCACTAC348180.46288141219644263No Hit
TTCAAGTAATTCAGGATAGGTT335600.4461571656416972No Hit
ACTGGACTTGGAGTCAGAAGGC313310.41652414054588843No Hit
CAACGGAATCCCAAAAGCAGCTGT312740.41576636466860667No Hit
CTTCTCACTACTGCACTTGACTAG283930.37746544708178514No Hit
TGTAAACATCCTACACTCTCAGCT280070.37233384201808745No Hit
TTCTCACTACTGCACTTGACTAG272530.36230992953614943No Hit
TGAGGTAGTAGATTGTATAGTT243310.3234639450902305No Hit
TAGCTTATCAGACTGATGTTGACA231920.3083217218582313No Hit
AACATTCAACGCTGTCGGTGAGTT231750.3080957185264104No Hit
CGCCCCACTGCTAAATTTGACTG220000.2924749000034964No Hit
TTTAAACATCCTTGACTGGAAGCT219600.2919431274580355No Hit
CAAAGAATTCTCCTTTTGGGCT202910.26975491799867934No Hit
CCCCCACTGCTAAATTTGACTGGCTT195360.25971771120310483No Hit
CATTGCACTTGTCTCGGTCTGA190780.25362891555757744No Hit
CTCCCACTGCTTCACTTGACTAG187660.2494810897029824No Hit
CCCCCCACTGCTAAATTTGACTGGCT174340.23177306393913438No Hit
CCCCCCACTGCTAAATTTGACTGG173160.23020433493002473No Hit
CCCATAAAGTAGAAAGCACT168720.22430165967540872No Hit
CAAAGAATTCTCCTTTTGGGCTT167510.22269304772538945No Hit
TGTCAGTTTGTCAAATACCCCAA164880.21919664323898402No Hit
TTCAAGTAATCCAGGATAGGCTT154780.20576938646609624No Hit
CCCGCCACTGCTAAATTTGACTG153100.20353594177516046No Hit
ACTTGACTTGGAGTCAGAAGGC140320.18654580894768463No Hit
ACCCCCCACTGCTAAATTTGACTG137230.18243786603399914No Hit
TAGCAGCACGTAAATATTGGC135210.1797524146794216No Hit
TGATGTAGTAGGTTGTATAGTT130050.17289254884297595No Hit
CTCCCACTGCTTCACTTGACTAGCCT129380.17200182982932893No Hit
CAACGGAATCCCAAAAGCAGCT123920.16474313458378761No Hit
CCCTCCACTGCTAAATTTGACTG122090.162310275188304No Hit
TAGCAGCACGTAAATATTGGCGT120230.1598375328519108No Hit
TGTCAGTTTGTCAAATACCCCA118860.15801621188370718No Hit
TTTAAACATCCCCGACTGGAAGCT115910.15409438936093303No Hit
CTACGCCTGTCTGAGCGTCGCT115800.15394815191093128No Hit
CGCCTGTCTGAGCGTCGCT115250.15321696466092255No Hit
TGTAAACATCCTACACTCAGCT112950.15015927252452235No Hit
TGTAAACATCCCCGACTGGAAGC112580.14966738291997103No Hit
ATCAGCATTGTACAGGGCTATGA109400.14543979118355685No Hit
CACCCGGCTGTGTGCACATGTGC108210.14385776786081067No Hit
CACTAGATTGTGAGCTCCTGGA107660.1431265806108019No Hit
TGCAAGTAATCCAGGATAGGCT105640.14044112925622437No Hit
GGAATACCGGGTGCTGTAGGCTTT105320.14001571121985565No Hit
TGAGGTAGTAGTTTGTACAGTT104870.1394174671062121No Hit
CAACACCAGTCGATGGGCTGT101170.13449857106069876No Hit
CACTAGATTGTGAGCTCCTGGAA100020.13296972499249868No Hit
TTCAAGTAATCCAGGATAGGC100020.13296972499249868No Hit
TTATAAAGCAATGAGACTGATT99690.13253101264249345No Hit
TATTGCACTTGTCCCGGCCTGTAG98920.1315073504924812No Hit
TATTGCACTTGTCCCGGCCTGTT97930.13019121344246548No Hit
GACCCGCGGGCGCTCTCCAGTCCT91910.12218803663327889No Hit
ATCACATTGCCAGGGATTTCCA91720.12193544467418496No Hit
GCCCCCACTGCTAAATTTGACTG91540.12169614702872754No Hit
TTCACAGTGGCTAAGTTCCG90340.12010082939234484No Hit
CGACGGAATCCCAAAAGCAGCTG89920.11954246821961088No Hit
AGCAGCATTGTACAGGGCTATGT89470.11894422410596737No Hit
TTTAAACATCCTACACTCTCAGCT86430.11490275276046452No Hit
GGAATACCGGGTGCTGTAGGCTT84940.11292190002862267No Hit
TCGTACGACTCTTAGCGG81380.10818912437402062No Hit
ACTGGACTTGGAGTCAGAAGGCA79850.10615509438763268No Hit
AACATTCAACGCTGTCGGTGA79620.10584932517399266No Hit
ACCACAGGGTAGAACCACGGAC79460.10563661615580829No Hit
TGATGTAGTAGATTGTATAGTT79120.10518460949216653No Hit
AACGGAATCCCAAAAGCAGCTG78500.10436036204670213No Hit
ATCACATTGCCAGGGATTTCC78100.10382858950124121No Hit
TGAGGTAGTAGTTTGTGCTGTT77260.10271186715577332No Hit
CTCCCCACTGCTAAATTTGACTG77240.10268527852850028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)