FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7659089
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG112742514.720092689874736No Hit
CAACGGAATCCCAAAAGCAGCTG7130459.309788670689164No Hit
CCCCCACTGCTAAATTTGACTG4018445.246629200940216No Hit
AACATTCAACGCTGTCGGTGAGT2346773.0640328112129263No Hit
AACATTCAACGCTGTCGGTGAG1856292.423643334083205No Hit
TTCAAGTAATCCAGGATAGGCT1731532.260751898822432No Hit
TAGCAGCACGTAAATATTGGCG1656312.162541785321988No Hit
TAGCTTATCAGACTGATGTTGAC1482221.9352432123454892No Hit
AAGCTGCCAGTTGAAGAACTGT1432491.8703138193067088No Hit
TAGCTTATCAGACTGATGTTGACT1368011.7861262612302846No Hit
TATTGCACTTGTCCCGGCCTGTAA1080091.4102068796954834No Hit
TAGCTTATCAGACTGATGTTGA1054831.3772264560445766No Hit
TATTGCACTTGTCCCGGCCTGTAT921781.2035112792134939No Hit
TGAGGTAGTAGGTTGTATAGTT619910.8093782432871585No Hit
TATTGCACTTGTCCCGGCCTGT573340.7485746673005106No Hit
TATTGCACTTGTCCCGGCCTGTA503220.6570233091690147No Hit
CATAAAGTAGAAAGCACTACT487770.6368511973160254No Hit
TGTAAACATCCTTGACTGGAAGCT463560.6052416938881373No Hit
AGCAGCATTGTACAGGGCTATGA387210.5055562091000639No Hit
TGAGGTAGTAGATTGTATAGTT378280.4938968590128669No Hit
TGTAAACATCCCCGACTGGAAGCT365110.4767016025012896No Hit
CAACGGAATCCCAAAAGCAGCTGT331910.43335441068774627No Hit
CAACGGAATCCCAAAAGCAGCT317550.41460544459008114No Hit
CATTGCACTTGTCTCGGTCTGA312230.40765944879345317No Hit
TTCAAGTAATTCAGGATAGGTT304890.3980760636153986No Hit
ACTGGACTTGGAGTCAGAAGGC266940.34852708984058023No Hit
CAACACCAGTCGATGGGCTGT238920.31194310446059575No Hit
CCCCCACTGCTAAATTTGACTGGCTT236960.30938405337762753No Hit
CACCCGGCTGTGTGCACATGTGC236060.3082089789007544No Hit
TTAATGCTAATCGTGATAGGGGTT228350.2981425075488743No Hit
CCCATAAAGTAGAAAGCACT227220.29666713626124463No Hit
TGATGTAGTAGGTTGTATAGTT220630.2880629798139178No Hit
TAGCTTATCAGACTGATGTTGACA214270.27975912017734744No Hit
TCGTACGACTCTTAGCGG213180.2783359744220233No Hit
CCCATAAAGTAGAAAGCACTAC198350.25897335831976886No Hit
TTCTCACTACTGCACTTGACTAG186330.24327958586197393No Hit
CGCCCCACTGCTAAATTTGACTG179380.23420539962389783No Hit
ACCCCCCACTGCTAAATTTGACTG178570.23314783259471197No Hit
CACTAGATTGTGAGCTCCTGGA175820.22955732724871067No Hit
CCCGCCACTGCTAAATTTGACTG169070.22074426867216193No Hit
CTCCCCACTGCTAAATTTGACTG164870.21526058778008716No Hit
CTTCTCACTACTGCACTTGACTAG156500.20433239514516677No Hit
ACCGGGTGCTGTAGGCTT156350.20413654939902123No Hit
CACTAGATTGTGAGCTCCTGGAA155320.20279174194215527No Hit
AACATTCAACGCTGTCGGTGAGTT150350.1963027195532001No Hit
TGTAAACATCCTACACTCTCAGCT141990.18538758330135607No Hit
AACGGAATCCCAAAAGCAGCTG139460.18208431838303485No Hit
CCCCCCACTGCTAAATTTGACTGG138350.18063505986155795No Hit
CTCCCACTGCTTCACTTGACTAG136990.17885939176317184No Hit
TAGCAGCACGTAAATATTGGC135640.17709678004786208No Hit
CAAAGAATTCTCCTTTTGGGCT135510.1769270470678693No Hit
TGATGTAGTAGATTGTATAGTT133770.17465523641258118No Hit
TGAGGTAGTAGTTTGTACAGTT133610.1744463342833593No Hit
TATTGCACTTGTCCCGGCCTGTAG132450.17293179384650056No Hit
CTCCCACTGCTTCACTTGACTAGCCT132120.1725009332049804No Hit
TCGTTTCCCGGCCAATGCACCA129690.16932823211742284No Hit
ACCGGGTGCTGTAGGCTTA117250.15308609157042044No Hit
CCCCCCACTGCTAAATTTGACTGGCT112240.14654484364915984No Hit
ACCGGGTGCTGTAGGCTTT110510.1442860893769481No Hit
TATTGCACTTGTCCCGGCCTGTT107200.13996442657867011No Hit
ACTTGACTTGGAGTCAGAAGGC103780.1354991435665521No Hit
TTATAAAGCAATGAGACTGATT103710.13540774888501753No Hit
TGAGGTAGTAGTTTGTGCTGTT103680.13536857973578842No Hit
TGTCAGTTTGTCAAATACCCCA103010.13449380206967174No Hit
TTCAAGTAATCCAGGATAGGCTT102490.13381487014970056No Hit
TGAGAACTGAATTCCATGGGTT102010.1331881637620349No Hit
TGTCAGTTTGTCAAATACCCCAA100240.13087718395751766No Hit
TGAGAACTGAATTCCATAGGCTGT98050.12801783606379297No Hit
TATTGCACTTGTCCCGGCCTGTAAT97710.12757391903919646No Hit
CAAAGAATTCTCCTTTTGGGCTT97300.12703860733306532No Hit
GCCCCCACTGCTAAATTTGACTG90320.11792525194576013No Hit
CCCATAAAGTAGAAAGCACTA88470.11550982107663196No Hit
TGCAAGTAATCCAGGATAGGCT87710.11451753596282796No Hit
GACCCGCGGGCGCTCTCCAGTCCT86810.11334246148595478No Hit
TCAGTGCACTACAGAACTTTGT85920.112180443392158No Hit
TGTAAACATCCCCGACTGGAAGC84980.11095314338297937No Hit
AGCAGCATTGTACAGGGCTATGT84800.11071812848760473No Hit
ACTGGACTTGGAGTCAGAAGGCT82740.10802851357387283No Hit
ACTGGACTTGGAGTCAGAAGGCA81410.1062920146247158No Hit
CAAGGGAATCCCAAAAGCAGCTG79640.10398103482019859No Hit
CCCCCCACTGCTAAATTTGACTGT79120.10330210290022744No Hit
TATTGCACTTGTCCCGGCCTGTAAA79030.10318459545254012No Hit
CTACGGAATCCCAAAAGCAGCTG78900.10301486247254732No Hit
CAACGGAATCCCAAAAGCAGCTGA78350.10229676140334706No Hit
CGACGGAATCCCAAAAGCAGCTG76830.10031219117573906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)