FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7516843
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG100824813.413184231731327No Hit
CAACGGAATCCCAAAAGCAGCTG6142198.17123624904764No Hit
CCCCCACTGCTAAATTTGACTG2675743.5596592878153768No Hit
AACATTCAACGCTGTCGGTGAGT2662683.5422849725609544No Hit
TAGCAGCACGTAAATATTGGCG2483933.304485673041196No Hit
TTCAAGTAATCCAGGATAGGCT2071312.75555841727704No Hit
AAGCTGCCAGTTGAAGAACTGT2044832.7203308623048263No Hit
AACATTCAACGCTGTCGGTGAG1628382.1663083823887237No Hit
TATTGCACTTGTCCCGGCCTGTAA1208541.6077760304425674No Hit
TAGCTTATCAGACTGATGTTGACT1193661.587980485956671No Hit
TAGCTTATCAGACTGATGTTGAC1116111.484812174472714No Hit
TATTGCACTTGTCCCGGCCTGTAT1074521.4294830954963407No Hit
TAGCTTATCAGACTGATGTTGA818361.0887017328950466No Hit
TGTAAACATCCTTGACTGGAAGCT628510.8361355957547603No Hit
TGAGGTAGTAGGTTGTATAGTT603720.8031563250689152No Hit
TATTGCACTTGTCCCGGCCTGT593440.7894803709482824No Hit
GGAATACCGGGTGCTGTAGGCTTT556670.74056355839812No Hit
TGTAAACATCCCCGACTGGAAGCT437070.5814542089012634No Hit
TGAGGTAGTAGATTGTATAGTT436420.5805894841757371No Hit
TATTGCACTTGTCCCGGCCTGTA430440.5726340167008942No Hit
TTCAAGTAATTCAGGATAGGTT413020.5494593940567869No Hit
GGAATACCGGGTGCTGTAGGCTT409440.5446967563377338No Hit
CATAAAGTAGAAAGCACTACT386420.5140721975967836No Hit
AGCAGCATTGTACAGGGCTATGA377990.5028573830795721No Hit
TTCTCACTACTGCACTTGACTAG375990.5001966916164139No Hit
CAACGGAATCCCAAAAGCAGCTGT343210.4565879585352521No Hit
CTTCTCACTACTGCACTTGACTAG310320.41283288742361657No Hit
TTAATGCTAATCGTGATAGGGGTT281710.3747716960431394No Hit
ACTGGACTTGGAGTCAGAAGGC256490.34122037669271527No Hit
CCCCCCACTGCTAAATTTGACTGG236450.31456024823187073No Hit
TGAATACCGGGTGCTGTAGGCTTT225770.3003521558186063No Hit
CTCCCACTGCTTCACTTGACTAG223690.2975850366969218No Hit
CCCCCACTGCTAAATTTGACTGGCTT221480.2946449726301321No Hit
TGAGAACTGAATTCCATGGGTT208810.27778949221102534No Hit
CCCATAAAGTAGAAAGCACTAC207380.27588709781486725No Hit
CACCCGGCTGTGTGCACATGTGC200850.2671999401876559No Hit
TGTAAACATCCTACACTCTCAGCT199050.26480531787081363No Hit
CATTGCACTTGTCTCGGTCTGA194860.2592311692554973No Hit
ACCCCCCACTGCTAAATTTGACTG192140.25561262886560226No Hit
GGGAATACCGGGTGCTGTAGGCTTT190650.2536304137255494No Hit
CCCATAAAGTAGAAAGCACT186800.24850858265897No Hit
CAAAGAATTCTCCTTTTGGGCT184590.24556851859218026No Hit
CAACGGAATCCCAAAAGCAGCT181390.24131141225112723No Hit
TAGCTTATCAGACTGATGTTGACA174440.23206550941665272No Hit
GGGAATACCGGGTGCTGTAGGCTT172710.22976401130102095No Hit
TGAATACCGGGTGCTGTAGGCTT169620.22565324299044157No Hit
TGATGTAGTAGGTTGTATAGTT169300.2252275323563363No Hit
TGAGGTAGTAGTTTGTACAGTT168210.2237774555089151No Hit
AACATTCAACGCTGTCGGTGAGTT163560.21759134785707246No Hit
GACCCGCGGGCGCTCTCCAGTCCT162080.2156224361743354No Hit
CCCCCCACTGCTAAATTTGACTGGCT151290.20126800573059728No Hit
CAAAGAATTCTCCTTTTGGGCTT145410.19344557282891237No Hit
TATTGCACTTGTCCCGGCCTGTAG143340.19069175716454367No Hit
TAGCAGCACGTAAATATTGGC139330.18535707078091163No Hit
TGTAAACATCCCCGACTGGAAGC137750.1832551245250167No Hit
TGAGAACTGAATTCCATAGGCTGT130250.17327753153817368No Hit
CACTAGATTGTGAGCTCCTGGA127140.16914015631296278No Hit
ACCGGGTGCTGTAGGCTTA125000.16629321644738357No Hit
AACGGAATCCCAAAAGCAGCTG123020.163659131898857No Hit
CACTAGATTGTGAGCTCCTGGAA121940.1622223585087516No Hit
AGCTCGGTCTGAGGCCCCTCAGT120550.1603731779418567No Hit
TGATGTAGTAGATTGTATAGTT119030.15835105242985653No Hit
CTCCCACTGCTTCACTTGACTAGCCT118660.15785882450917227No Hit
TATTGCACTTGTCCCGGCCTGTT115420.15354850433885608No Hit
TTCAAGTAATCCAGGATAGGCTT114810.15273699344259287No Hit
TGAGGTAGTAGTTTGTGCTGTT114040.15171262722927698No Hit
ACCGGGTGCTGTAGGCTT109180.1452471469738027No Hit
CAACACCAGTCGATGGGCTGT108520.1443691187909605No Hit
TATTGCACTTGTCCCGGCCTGTAAT104850.13948674995606533No Hit
GGAATACCGGGTGCTGTAGGCTTA103180.1372650725843283No Hit
TGTCAGTTTGTCAAATACCCCA100430.13360662182248584No Hit
CGCGACCTCAGATCAGACGTGGCG99420.132262972633591No Hit
TATTGCACTTGTCCCGGCCTGTTT92020.12241841421990589No Hit
CCCCCCACTGCTAAATTTGACTGT90440.12031646796401096No Hit
ACCGGGTGCTGTAGGCTTT90410.12027655759206358No Hit
TGTCAGTTTGTCAAATACCCCAA87980.11704381746432645No Hit
GAATACCGGGTGCTGTAGGCTTT84780.11278671112327343No Hit
CTACGCCTGTCTGAGCGTCGCT83840.11153618613558909No Hit
TATTGCACTTGTCCCGGCCTGTAAA83190.1106714614100627No Hit
TGGAATACCGGGTGCTGTAGGCTTT82170.10931450876385206No Hit
AACATTCAACGCTGTCGGTGA81510.10843648058100988No Hit
TTCAAGTAATCCAGGATAGGC79910.10630792741048337No Hit
CGCCTGTCTGAGCGTCGCT78000.10376696706316735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)