FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7859058
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG119639415.223122160442129No Hit
CAACGGAATCCCAAAAGCAGCTG6848248.713817864685563No Hit
CCCCCACTGCTAAATTTGACTG3515004.472546200829667No Hit
TTCAAGTAATCCAGGATAGGCT2264962.8819738955991925No Hit
AACATTCAACGCTGTCGGTGAGT1949592.4806917063088223No Hit
TAGCAGCACGTAAATATTGGCG1719562.1879975946226633No Hit
AAGCTGCCAGTTGAAGAACTGT1630762.075006953759598No Hit
AACATTCAACGCTGTCGGTGAG1619352.0604886743423956No Hit
TATTGCACTTGTCCCGGCCTGTAT1152971.4670587747284727No Hit
TAGCTTATCAGACTGATGTTGACT1022831.3014664098419937No Hit
TATTGCACTTGTCCCGGCCTGTAA1013221.2892384812530968No Hit
TAGCTTATCAGACTGATGTTGAC955381.215641874636884No Hit
TATTGCACTTGTCCCGGCCTGT737700.9386621144671536No Hit
TAGCTTATCAGACTGATGTTGA722020.9187106139183603No Hit
TGAGGTAGTAGGTTGTATAGTT654110.8323007668349057No Hit
TATTGCACTTGTCCCGGCCTGTA629160.8005539595203395No Hit
TTCAAGTAATTCAGGATAGGTT457560.5822071805552269No Hit
ACCGGGTGCTGTAGGCTTA450730.5735165715789348No Hit
TTAATGCTAATCGTGATAGGGGTT446550.5681978679887589No Hit
TGAGGTAGTAGATTGTATAGTT444110.5650931701992783No Hit
TGTAAACATCCTTGACTGGAAGCT424270.5398484144028457No Hit
CATAAAGTAGAAAGCACTACT386310.49154746026813906No Hit
ACCGGGTGCTGTAGGCTT372020.47336461952564796No Hit
CAACGGAATCCCAAAAGCAGCTGT332340.42287510793278277No Hit
AGCAGCATTGTACAGGGCTATGA325350.41398091221619693No Hit
TGTAAACATCCCCGACTGGAAGCT285430.36318602051289095No Hit
CTCCCACTGCTTCACTTGACTAG275380.35039822838818596No Hit
ACCGGGTGCTGTAGGCTTT275330.35033460753184414No Hit
GCCTGTCTGAGCGTCGCT265680.33805578225787364No Hit
CATTGCACTTGTCTCGGTCTGA256220.3260187162380021No Hit
CCCATAAAGTAGAAAGCACT237620.3023517576788465No Hit
TCGTACGACTCTTAGCGG219800.2796772844786233No Hit
CCCCCACTGCTAAATTTGACTGGCTT216840.27591092978318776No Hit
ACCCCCCACTGCTAAATTTGACTG215290.27393868323659143No Hit
TTCTCACTACTGCACTTGACTAG208550.2653625918017146No Hit
ACTGGACTTGGAGTCAGAAGGC208440.26522262591776263No Hit
CAACGGAATCCCAAAAGCAGCT206320.26252510160886966No Hit
CCCATAAAGTAGAAAGCACTAC190290.24212825506568347No Hit
CACCCGGCTGTGTGCACATGTGC187060.23801834774600214No Hit
CAACACCAGTCGATGGGCTGT177720.2261339717813509No Hit
TATTGCACTTGTCCCGGCCTGTT164990.20993610175672456No Hit
CACTAGATTGTGAGCTCCTGGA163720.2083201320056424No Hit
CACTAGATTGTGAGCTCCTGGAA159720.2032304634982971No Hit
CAAAGAATTCTCCTTTTGGGCT156860.19959135051554525No Hit
CGCCCCACTGCTAAATTTGACTG155150.19741551722865514No Hit
CCCCCCACTGCTAAATTTGACTGG154170.19616854844435555No Hit
TATTGCACTTGTCCCGGCCTGTAG151470.19273302220189747No Hit
TGTAAACATCCTACACTCTCAGCT150900.19200774443960078No Hit
CCCCCCACTGCTAAATTTGACTGGCT150510.19151150176013462No Hit
AACGGAATCCCAAAAGCAGCTG150340.19129519084857244No Hit
GACCCGCGGGCGCTCTCCAGTCCT149970.19082439651164299No Hit
TGAGGTAGTAGTTTGTACAGTT148910.1894756343571965No Hit
CTTCTCACTACTGCACTTGACTAG148850.18939928932958633No Hit
TAGCTTATCAGACTGATGTTGACA144860.18432234499350938No Hit
ATCCCGGACGAGCCCCCA132050.16802268159873615No Hit
AACATTCAACGCTGTCGGTGAGTT131680.1675518872618067No Hit
CCCGCCACTGCTAAATTTGACTG127730.16252583961080322No Hit
CTCCCACTGCTTCACTTGACTAGCCT125940.1602482129537662No Hit
CCCCCCACTGCTAAATTTGACTGT117960.15009432428161237No Hit
AGCTCGGTCTGAGGCCCCTCAGT115320.1467351430667645No Hit
TAGCAGCACGTAAATATTGGC113160.14398672207279806No Hit
TGAGGTAGTAGTTTGTGCTGTT109870.13980046972550655No Hit
TATTGCACTTGTCCCGGCCTGTTT107770.13712839375915029No Hit
TCCTGTCTGAGCGTCGCT107170.13636494348304848No Hit
TGATGTAGTAGGTTGTATAGTT105140.13378193671557073No Hit
TGAGAACTGAATTCCATGGGTT100090.12735623022504733No Hit
TTCAAGTAATCCAGGATAGGCTT99110.12610926144074772No Hit
TTCAAGTAATCCAGGATAGGC98400.12520584528069395No Hit
AACCGGGCGGAAACACCA97590.12417518740795652No Hit
CCCCCCACTGCTAAATTTGACTGA96950.12336084044678128No Hit
TTCACAGTGGCTAAGTTCCG92570.11778765343123819No Hit
ACCTGGTGCTGTAGGCTTA91330.11620985619396117No Hit
TTCACAGTGGCTAAGTTCTG85040.1082063524661607No Hit
TCGTTTCCCGGGCGGCGCACCA81440.10362565080954994No Hit
TCAGTGCACTACAGAACTTTGT80480.10240413036778709No Hit
CCCATAAAGTAGAAAGCACTA80150.10198423271593109No Hit
TATTGCACTTGTCCCGGCCTGTAAT79910.1016788526054904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)